标本

Methods:The expressions of TGF-β1 and TGF-β2 were examined in surgical specimens of 84 patients with lung carci noma by SABC immunohistochemistry.

用SABC免疫组化染色法检测84例肺癌组织标本中TGF-β的表达。

Methods Bcl-2and VEGF were detected immunohistochemically in92cases of colorectal adeˉnoma with mild,severe abnormal hyperplastic and canceration areas.

采用免疫组化SP法检测92例大肠腺瘤癌变标本中bcl-2和VEGF在腺瘤轻、重度异型增生区及癌变区表达。

Results Of the 109 cases,the spots of 45 cases platelet volume,platelet quantity on rectangular coordinate chart were outside of the platelet nomogram further more a majority of them were below the platelet nomogram.

结果 109例正常老年人标本中有45例血小板数量与血小板体积在直角坐标图上的点位于逆方图外且大部分位于逆方图下方。

METHODS: A total of 264 chronic HBVinfected patients were enrolled in our study, and were divided into lowlevel HBsAg group(147 cases) and highlevel HBsAg group(117 cases) based on the HBsAg level or were divided into immune tolerance stage, immune clearance stage and nonactive stage based on the natural history. Realtime PCR and microparticle enzyme immunoassay were used to determine the content of HBV DNA and HBV M in serum samples of highlevel and lowlevel HBsAg patients, respectively, then the quantitative results were compared.

对264例慢性HBV感染者的HBsAg浓度(高浓度HBsAg组、低浓度HBsAg组)及自然史(免疫耐受期、免疫清除期、非活动期)进行分组，采用实时荧光聚合酶链反应和微粒子酶免疫试验对147例低浓度HBsA组和117例高浓度HBsAg组的血清标本进行HBV DNA和HBV M的定量测定并进行比较。

In this report, we analyzed the cellular and noncellular findings of bronchoalveolar lavage fluid in 18 cases with IPF, 12 patients with Sar and 9 healthy volunteers as conirols, using immunohistochemicat staining, we also have estimted collagen typeⅠ and type Ⅲ semiquantitatively in transbronchial lung biopsy specimens from 25 patients with IPF and 10 normal lung tissue. The aim of this investigation is to select the criteria for evaluating the disease actiity.

本文对18例IPF、12例Sar和9例正常对照者的支气管肺泡灌洗液中细胞成份和非细胞成份进行了检测和分析，同时对10例正常肺和25例IPF患者经支气管肺活检的肺组织标本，应用免疫组化方法、半定量剂检测其中Ⅰ型胶原和Ⅲ型胶原的含量结合临床治疗效果，对有助于疾病活动性判断的指标进行了筛选和进一步的评价。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

Methods The expressions of p16 and Ki-67 in preoperative samples, recurrent samples and nonrecurrent samples were detected with SP immunohistochemical staining method, respectively.

SP法检测p16、Ki-67在尖锐湿疣患者术前、术后复发及未复发者标本中的表达。

METHODS: The label index of Ki-67 was studied by immunohistochemical method in the tissue of superficial lobe parotidectomy which included the primary and secondary operation cases, nonrecurrent group, and group of low-grade malignant parotid gland tumors.

运用免疫组织化学方法，测定并比较细胞增殖活性相关抗原Ki-67在腮腺多形性腺瘤腮腺浅叶切除术后未复发病例、复发前病例、复发后病例及腮腺低度恶性肿瘤病例术后石蜡标本中的阳性表达率。

We study the expression levels of GLUT1 in 16 samples of esophageal cancer and their normal mucosa by reverse transcriptasepolymerase chain reaction and immunohistochemical staining, analyse the correlation between the expression level of GLUT1 mRNA and protein in the tumor cells. Then we study the expression levels of GLUT1 in 55 patients with esophageal cancer by immnohistochemical staining, analyse the relationship between GLUT1 expression and clinical features of the patients.

我们采用逆转录聚合酶链反应和免疫组化染色的方法，研究GLUT1 mRNA及蛋白在16例食管癌病人的肿瘤组织和相应正常食管粘膜的表达水平，同时分析食管癌组织中GLUT1在mRNA水平和蛋白水平表达的相关性；采用免疫组化染色的方法研究55例食管癌病人肿瘤标本的GLUT1表达水平，并分析其与肿瘤的临床特性及预后的关系。

It is helpful for understanding its pathogenesis and manifestation to know the pathological character of chronic prostatitis. It is significant for the knowledge and treatment choice of chronic prostatitis to know whether or not inflammation exist in normal prostate and its character.

了解慢性前列腺炎的病理特点对于认识和理解疾病的发病机理及临床表现有重要的帮助，而了解正常前列腺标本中炎症的有无及分布特点对于慢性前列腺炎的认识及治疗方法的选择有意义。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐