标本

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

Based on examinations of type specimens housed at PE, lectotypes for five names of Chinese taxa in Fagaceae, Papaveraceae, Capparidaceae and Cruciferae are here designated under Article 8.1, 9.9, 9.10 and 37.2, Recommendation 9A.2 and 9A.3 of the International Code of Botanical Nomenclature, because the types were respectively indicated by reference to a gathering that consists of more than one specimen, or more than one gathering were simultaneously designated, without indicating the type in the protologue.

摘要查阅保存于中国科学院植物研究所标本馆的壳斗科、罂粟科、山柑科和十字花科植物的模式标本时，发现有5个植物名称的模式均多于1份标本，或同时引证1号以上的标本而没指定模式，根据《国际植物命名法规》规则8.1、9.9、9.10和37.2，以及辅则9A.2和9A.3的精神，对这些名称做出后选模式指定。关键词壳斗科；罂粟科；山柑科；十字花科；后选模式

Methods compare with sysmex-r3500 analyzer、coulter maxm analyzer、miller ocularto determine 130 ordinary samples.results f=3.87,p.05,the difference is conspicuous.each"r"is 0.7331,0.7231,0.5011,each other are beeline correlation.about sysmex-r3500 analyzer,the inter-assay cv of quality control cv=2.95%,the sample is cv=4.42%;about couter maxm analyzer,the intra-assay cv is 5.70%,intra-assay cv is 17.02%;about miller ocular,the inter-assay cv is 26.15%.conclusion sysmex-r3500 analyzer is good at repeatability,convenience,quickness,so it is adapt to clinical batch samples quickly determine.

分别用sysmex-r3500分析仪，coulter maxm分析仪以及国际血液标准化委员会所推荐的miller窥盘法进行比较，对130份正常人标本进行网织红细胞测定。结果：f=3.87，p.05，差异有显著性。其两两之间r值分别是0.7331，0.7231，0.5011，两两之间直线相关。sysmex-r3500分析仪的质控物批内平均cv=2.95%，标本批内平均cv=4.42%，批间平均cv=3.42%；coulter maxm分析仪批内平均cv=5.70%，批间平均cv=17.02%；miller窥盘法批内平均cv=26.15%。结论：sysmex-r3500分析仪具有重复性好，简便，快捷的特点，尤其适合临床大批标本的快速测定。网织红细胞 miller；窥盘法；仪器法

Hematoxylin and eosin, Alcian blue, Masson Trichrome, and Elastica Van Giesonstains were performed for each LF. Nine LF were collected and were used for biologic study of real time RT-PCR to quantitatively measure mRNA expression of Type I collagen and elastin in each LF.

每份标本用HE染色、Alcian蓝染色， Masson Trichrome染色以及Elastica Van Gieson染色。9份标本染色后收集，用实时定量PCR定量分析每份标本中I 型胶原纤维和弹力纤维的mRNA的表达。

I examined the specimens deposited at PE, KUN, IBK, IBSC, and HIB. And I required to take a lot of photos of type specimens in Harvard University Herbarium and several photos of type specimens in the herbaria in China, or downloaded some photos of type specimens at the website of NY, K, US, and HK. I went to Yunnan, Chongqing, Guizhou, Hubei, Hunan, Guanxi to collect specimens and observed the kiwifruits cultivated in Wuhan Botanical Garden and Guangxi Institute of Botany. With reference to the results of previous authors, I recognized 52 species including 16 varieties of Actinidia.

作者查阅了中国科学院植物研究所国家植物标本馆、昆明植物研究所标本馆、广西植物研究所标本馆、华南植物园标本馆、武汉植物园标本馆的标本，并请求哈佛大学同行拍摄了大量保存在国外的模式标本的照片，从国外和国内一些标本馆相关的网站上下载一些模式照片，请求国内的同行帮助拍摄了少数模式标本的照片，到云南、重庆、湖北、湖南、广西、贵州进行野外考察，在武汉植物园和广西植物所的猕猴桃资源圃中对栽培的猕猴桃属植物进行观察，结合文献资料，对猕猴桃属植物的分类进行了全面的研究。

Out of all P16 gene changes,4 were dot mutation,2 were deletion and 1 was heterozygous.

结果16例胰腺组织标本中有7例检测出存在着P16基因的改变，4例标本为点突变，2例标本为缺失型改变，还有1例患者存在着野生型与缺失型同时存在的杂和状态。

The Linnaean collection of the Herbarium contains the proposed lectotype of a species described by Linnaeus as well as iso-lectotypes and original material for several other Linnaean plant names.

该馆收藏的林奈标本包括林奈所描述物种的选模式标本，同源选模式标本和其他几种林奈植物名称的原始材料。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

Methods From January to March 2007, cases from both outbreaks and sporadic episodes of acute nonbacterial gastroenteritis were investigated in Beijing, and the fecal specimens of the patients were collected.

方法收集北京市2007年1—3月非细菌性胃肠炎暴发和散发病例，采集患者粪便标本，使用逆转录聚合酶链反应对粪便标本进行诺如病毒RNA检测，对RT—PCR阳性标本的PCR产物进行克隆测序。

Oncosis cells were found in all samples, three nuclear cataract and four cortical cataract had paraptosis cells, but only one sample had apoptosis cell.

所有标本均发现胀亡细胞，3例核性白内障及4例皮质性白内障标本发现典型paraptosis样细胞，仅有1例核性白内障标本发现早期凋亡细胞，细胞内偶见髓样体存在。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。