标本

By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法，以Roche试剂盒为标准，比较两公司的DNA聚合酶，用鼠疫菌310 0 4建立荧光PCR反应体系；针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物，检测其灵敏度和特异性，以盲测试验进行验证；在此基础上鉴定2 75株鼠疫耶尔森氏菌，并测试该法检测脏器污染模拟标本的灵敏度。

Methods Separate and distill DNA of the samples of 71 chronic cervicitis patients,76 CIN patients and 36 cervical cancer patients,to detect the cymene status of P16 gene with methylic differential nestPCR test.

分离提取71例慢性宫颈炎患者，76例CIN患者， 36例宫颈癌患者标本中的DNA，采用巢式甲基化特异性PCR方法检测P16基因的甲基化状态。

The purpose of this study is to investigate the frequency of prostate cancer discovered incidentally in radical cystoprostatectomy specimens in Asia and to determine the feasibility of prostate-sparing cystectomy for Asian patients.

本研究的目的是调查亚洲人群中全膀胱切除标本中前列腺偶发癌的发生率，并进一步探讨保留前列腺的膀胱切除术是否适合于亚洲患者。

Methods 25 patients with upper tract transitional cell carcinoma underwent resection under Laparoscope and cystoscope. Integrity and the detent of ureterostoma was detected after the operation.

对25例肾盂肿瘤或中上段输尿管肿瘤的患者采取腹腔镜联合膀胱镜行肾、输尿管全长切除，术后检查手术标本的完整性，并行输尿管内压力测定，对输尿管口的封闭性进行评估。

Due to the breakthrough of stained technology in 1970s, the band-pattern of chromosome provides material visual information for cytogeneticist, then analyses the composition of genome in a cell and measures the features of the equipments for karyotyping.In order to meet the demands of clinical hospital, a chromosome analysis system based on PC is developed.

由于70年代标本染色技术的突破(取代了此前的均匀染色方法，产生了染色体的带型特征)，染色体的带型模式为细胞遗传学家提供了实质性的视觉信息，从而可以进一步分析细胞的基因组的合成以及进行自动核型分类仪器中的特征测量。

Specimen preparation , a crucial step , is usually properly done if a cytologist is present during the procedure

标本的制备是一个关键的步骤，如果细胞学者在操作现场者则通常由其完成。

Objective To study the application of dropsy of serous cavity in examination of tumor cell.Methods The dregs in gray stratum been spread on a slide and staimed with hematoxyllin/eosine,the microscopic examination identified by professional cytologist and pathologist.

目的 探讨浆膜腔积液在检测肿瘤细胞中的应用价值方法取浆膜腔积液以3000转/min离心标本5min，取灰白膜层沉淀涂片，用苏木素-伊红染色镜检。

Methods The dregs in gray stratum been spread on a slide and staimed with hematoxyllin/eosine,the microscopic examination identified by professional cytologist and pathologist.

方法取浆膜腔积液以3转/min离心标本5min，取灰白膜目的探讨浆膜腔积液在检测肿瘤细胞中的应用价值。

Methods dregs in gray stratum been spread on a slide and staimed with hematoxyllin/eosine,the microscopic examination identified by professional cytologist and pathologist.

取浆膜腔积液以3000转/min离心标本5min，取灰白膜层沉淀涂片，用苏木素-伊红染色镜检。

HP were determined by rapid urease test,Warthin-Starry silver stain and polyme rase chain reaction,and expression of P21,P53,C-myc and C-erbB-2 were detecte d by immunohistochemicology (Streptaovidin/Peroxidase,SP) method,and were evalua ted with positive expressive integrality,the mean optical density an d the integral optical density in computer assisted image analysis system.

方法经内镜和病理诊断的胃癌91例，用快速尿素酶试验，改良W-S银染色和PCR方法检测上述标本中Hp；以S-P免疫组织化学法检测P21、P5 3、C-mcy及C-erbB-2，并用阳性表达积分半定量和免疫组化图像分析进行比较。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。