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Methods: Twenty-four rabbits were randomly divided into three groups:① the control group(group C, n=8): received sternotomy only and no ischemia;② the ischemia/reperfusion group(group I/R, n=8): the left lungs of rabbits were rendered ischemia by ligating the left pulmonary hili for 60 minutes followed by 60 minutes reperfusion;③ the edaravone group(group E, n=8): the left lungs were rendered ischemia for 60 minutes followed by 60 minutes reperfusion, and 10 mg/kg edaravone was administered intravenously 5 minutes prior to ischemia. Blood MDA and SOD were measured. Protein concentrations, WBC count, and PMN percentage in broncho-alveolar lavage fluid were determined. Samples of left lung tissue were sent for determining the left lung dry-to-wet weight ratio and evaluating the pathologic changes.

采用兔肺原位热缺血再灌注损伤模型进行研究。24只大白兔随机分为三组:①对照组(C组, n=8),开胸后不阻断肺门,静脉缓慢推注生理盐水5ml/kg;②缺血再灌注组(I/R组, n=8),左肺接受60min的缺血,然后接受60min再灌注,于缺血前5min静脉缓慢推注生理盐水5ml/kg;③依达拉奉干预组(E组, n=8),于缺血前5min静脉缓慢推注依达拉奉10mg/kg(5ml/kg),左肺接受60min的缺血,然后接受60min再灌注。120min实验结束时,留取各组动物血液标本,测定血浆丙二醛含量及超氧化物歧化酶活性;收集支气管肺泡灌洗液,检测支气管肺泡灌洗液中白细胞计数、中性粒细胞百分比及肺通透性指数;留取肺组织标本,测定肺组织湿、干重量,计算干/湿重比及行肺组织病理检查。

The lingual nerve and its neigh-bouring structures were investigatedon 33 sides of head-neck specimensand 61 mandibulae .

在33侧成人头颈部标本和61块下颌骨标本上对舌神经在下颌第三磨牙舌侧区的位置及其与周围结构的毗邻关系进行了观测。

The lingual nerve and its neigh-bouring structures were investigatedon 33 sides of head-neck specimensand 61 mandibulae.At median line ofthe third molar,the distance betweenthe lingual nerve and the gingive me-asures 11.7mm.

在33侧成人头颈部标本和61块下颌骨标本上对舌神经在下颌第三磨牙舌侧区的位置及其与周围结构的毗邻关系进行了观测。

Methods: forty seven cases of nasal nk/t cell lymphomas were studied by using methylation specific pcr.

选用12例已经确诊的鼻nk/t细胞淋巴瘤的冰冻组织标本和35例石蜡组织标本

Good chromosome preparation methods are necessary to get good spreading of chromosomes within metaphases for karyotype analysis.

染色体标本的制备是核型分析最基础的工作,要制作高质量的染色体标本,获得可靠的镜检结果,必须要有很好的制片方法。

The sixth, there were no significantly differences between colchicine groupsand vinblastine groups on the metaphases, in the experiment of prepare mouse4-cell embryo stage single blastomere chromosome specimen by using optimumparameters; and there were significantly differences between colchicine groupsand vinblastine groups on the metaphases (P<0.05), in the experiment of preparemouse 8-cell embryo stage single blastomere chromosome specimen by usingoptimum parameters.The end, the preparation quality of mouse 4-and 8-cell embryo stage singleblastomere specimen were 2.5, 2.7, 2.4, 1.9, respectively.

低渗液选用0.45%氯化钠时,低渗效果优于选用0.5%柠檬酸钠和0.35%氯化钾的。6、在利用最佳参数制备小鼠4-细胞期胚胎单卵裂球染色体标本时,经统计分析,两种阻断剂的作用效果差异不显著;而在制备小鼠8-细胞期胚胎单卵裂球染色体时阻断剂选用长春花碱的效果优于秋水仙素的(P<0.05)。7、秋水仙素组和长春花碱组小鼠4、8-细胞胚胎单卵裂球染色体标本制备质量分别为:2.5、2.7、2.4、1.9。

Methods MSCs were cultured with inbred line small-ear cancellous bone of Ban Na pigs . A 1.5 centimeter segmental defect was created in the mid-upper part of the radial shaft of adult rabbit. The defects were implanted into composited xenogeneic bone in experimental group and xenogeneic bone alone in control group , the defects were not filled with anything in blank group . The repair capability of the defects was assessed by scan electron microscope before operation and physical , histology, X-ray , transmission electron microscope, SPECT and bone mineral density examinations 4 , 8 and 12 weeks after operation .

将MSCs与版纳近交系小耳猪松质骨在体外联合培养,兔桡骨中上段制成1.5cm的骨—骨膜缺损模型,实验组植入复合异种骨,对照组植入单纯异种骨,空白对照组不植入任何材料,分别于术前行标本的扫描电镜观察和术后4周,8周,12周各时间点行标本的大体观察,组织学观察,X线片观察,透射电镜观察,SPECT扫描及骨密度测试,比较其骨缺损区骨修复愈合情况。

The gene typings of the bacterial from lower respiratory tract secretion and gastric aspirates were taken by plasmial profile analysis,restriction andonuclease fingerprint analysis and Genome Macrorestriction Pulsed Field Gel Electrophoresis in the same phenotype bacteria.

以气管切开或插管患者为研究对象,用防污染标本刷采集标本,由细菌表型分型,直至质粒DNA图谱、酶切图谱、全基因组DNA稀有位点酶切脉冲电场电泳(genome macrorestriction pulsed field gel electrophosresis, GM PFGE)等基因分型方法,前瞻性观察NP的发生情况。

Eighteen tibia specimens were divided into three groups randomly, all prostheses were cemented onto the medial compartment of the tibias.

将18具人体胫骨标本随机分为三组,把三种假体牢固地安装在三组标本的内侧室。

objective to study the infection and sensitivity to antimicrobial agents of staphylococcus of bacterial prostatitis patients.methods the results of bacteriology and drug susceptibility of 421 patients,who were suffered from prostatitis,were analysed retrospectively.results in the 421 succus prostaticus samples,278 strains pathogenic microorganisms of 15 genera were isolated from the 273 succus prostaticus samples,and the major pathogenic microorganisms was staphylococcus aureus(57.2%) and coagulase negative staphylocous(17.3%.the drug resistance rate of staphylococcus to penicilling,erythromycin and albomycin was 88%-97%,and it was 33%-50% to fluoroquinolones.the drug resistance rate of staphylococcus to amikacin,cefazolin,nemycin,rifampicin and cefoperazone was lower than 30%,and they all appeared susceptible to vancomycin.conclusion staphylococcus is the main germ of bacterial prostatitis and it appears high drug resistance to usual antibacterials.staphylococcus is susceptible to vancomycin,amikacin,cefazolin,neomycin,rifampicin and cefoperazone.

目的 探讨细菌性前列腺炎患者葡萄球菌的感染状况及抗菌药物敏感性。方法回顾分析421例前列腺炎患者标本的细菌学和药物敏感性试验结果。结果 421例前列腺液标本,273例分离出细菌,共分离出15个种别共计278株病原菌,其中金黄色葡萄球菌占57.2%,凝固酶阴性葡萄球菌占17.3%;葡萄球菌对青霉素g、红霉素、白霉素耐药率为88%~97%,对氟喹诺酮类耐药率为33%~50%,对丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮耐药率均0%,对万古霉素全部显示敏感。结论葡萄球菌是细菌性前列腺炎的最常见病原菌,对临床常用抗菌药物具有较高的耐药性,葡萄球菌对万古霉素、丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮较为敏感。

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