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Methods: The nose, nasal septum, sphenopalatine artery, anterior region of the aperture of sphenoidal sinus, sphenopalatine artery and adjacent structures of hypophysial fossa were dissected, observed, measured and photographed in different operational angle under the operation microscope by use 15 adult cadaveric heads and 20 adult cranial bones.

用15例(30侧)成人头颅标本及20侧(40侧)成人骨性标本,模拟手术角度,在手术显微镜下对鼻、鼻中隔、蝶窦开口前区、蝶腭动脉及垂体窝周围的结构等进行直接解剖、观察和测量,同时行显微摄像。

Methods: The fresh specimens of IgA nephropathy and normal control werepreserved in RNAwait in hypothermy, then the total RNA was extracted and detected.

收集3例IgA肾病肾穿组织标本和1例尸肾组织标本,应用RNAwait低温保存后提取总RNA,分别标记荧光探针后与含22575个基因的Agilent Human 1A oligo基因表达谱芯片杂交。

On the basis of past three years research, the taxonomy and fauna of family Ichneumonidae from northeast China were studied systematically, many specimens were collected in forest area from northeast China including Heilongjiang, Jilin and Liaoning Province, and numerous documents were synthesized and the domestic and foreign research history and situation of Ichneumonid were reviewed.

近三年来,广泛采集了东北地区(黑龙江、吉林、辽宁三省)的姬蜂标本,收集整理了大量文献资料,系统研究了东北地区姬蜂的种类及分布,回顾了国内外的研究历史和现状及分类地位的历史变革,介绍了标本采集制作方法,叙述了各虫态的形态特征,概述了成虫的寄主范围、寄生类型、寄生现象及生活习性。

We tried to probe into the relationships between survivin or P53 and the main clinicial characterstics and the safe incisal range of removing laryngeal squamous cell carcinoma according to the level of protein of gene expression .

研究喉鳞癌及癌旁组织中survivin、P53的表达情况,从基因蛋白水平角度探讨其与喉鳞癌的临床特征的关系以及手术安全切缘范围,我们对38例喉癌手术标本,10例声带息肉标本进行了研究。

Objective: The convenient, practical and innoxious technology to preserve the red-flower specimen was established by making the carnation as the object.

目的:以康乃馨为研究对象,建立简便易行、无毒的红色花标本保存技术,使红色花标本的颜色、形态接近自然状态,便于观赏和学习。

The occurrence rate of fissure and morphology of interlobar surface were observed on 45 right lung specimens.

在成人右肺标本45例中,取20例在肺门的4个层面上作常规CT扫描,观察其中12例的CT图像特征,并与标本相对照。

Methods:To observe the courser and adjacent relationship of the vascular nerve in gluteal region, vastus lateralis region and deltoid region on 20 infant corpses and 4 fault specimens.

对20具婴幼儿尸体标本和4套幼儿断层标本的臀区、股外侧区和三角肌区的血管神经走行和毗邻关系进行解剖观察,测量各区皮肤、浅筋膜和肌的厚度。

Take the blood specimen to measure the hepatic and renal function and the tissue biopsy after the active and motive abnormities called toxical manifestation such as posture changes, abnormal sound, uneasiness, quiet, thrill, ataxia, convulsion and so on with toxic dose. Measure the tissue biopsy after death with lethiferous dose. If no toxicity or death occur after the experiment of 12 weeks, measure the hepatic and renal function and the tissue biopsy. There was no rat which had the toxical manifestation such as active or motive abnormities and 3 rats died because of environment or others after the experiment of 12 weeks.

实验中分别测定治疗剂量组小鼠在用药4周,8周,12周后的肝肾功能,并进行组织病理检查;中毒剂量组小鼠在出现行为、动作异常,如改变姿势、叫声异常、不安、安静、震颤、运动失调、惊厥等中毒表现时,取血液标本测定肝肾功能,并进行组织病理检查;致死剂量组小鼠发生死亡后进行组织病理检查;若12周实验结束时无小鼠出现中毒表现或死亡,则取血液标本测定肝肾功能,并进行组织病理检查。12周实验结束时,无小鼠出现动作、行为异常等中毒表现,有3只小鼠因为环境或其它原因发生死亡,病理检查结果正常。

The HPV positivity amongst the group of women with no cervical abnormality was 43%, while that in women with cervical dysplasia and cervical cancer was 81.2% PCR products from 89 HPV positive cases were genotyped by LiPA, of which 49 cases were single HPV infected concerning 11 different HPV types and the commonest type was HPV-16, occurring in 25(51%) of all single HPV infected cases. The other 40 specimens contained multiple HPV types comprising 16 different HPV types. Although double infections(n=25) prevailed, triple(n=9),quadruple(n=3) and quintuple(n=3) infections were also found. PCR products of four HPV positive cases failed to be classified by LiPA. Sequencing analysis revealed that these 4 cases were HPV-66 which was not included in LiPA probes. Follow-up data of 12 cases confirmed the HPV genotyping results. Conclusion The LiPA for HPV genotyping is easy to perform.

结果 155例妇女子宫颈刮片中HPV DNA检出率为60%,其中在宫颈细胞学检查正常或仅有炎症病变的妇女中HPV DNA阳性率为43%,而在宫颈细胞增生不良或宫颈细胞癌的妇女其阳性率为81.2%。93例HPV阳性的标本中,89例利用LiPA成功地作出了基因分型,其中单独一型HPV感染为49例,涉及11种不同基因型HPV,最常见的型别为HPV 16,占单独感染的51%(25/49);另40例为不同型别HPV重叠感染,涉及所有16种HPV基因型别,二重感染占主导地位(n=25),同时也检测出其它多重感染。4例HPV阳性的标本应用LiPA未能作出基因分型,DNA序列分析显示这4例为HPV 66感染,此型别不包括在LiPA所标记的探针内。12例随诊资料显示了LiPA的物异性及高度的重复性。

METHODS: CD44v6 protein was detected by SP immunohistochemical method in the tissues of rectal cancer, mesorectum (2 cm distal from the tumor), and distal mesorectal margin (5 cm distal from the tumor) from 47 rectal cancer patients received total mesorectal excision. RESULTS: CD44v6 was highly expressed in the tissues of rectal cancer.

行全直肠系膜切除术的直肠癌患者47例于直肠癌组织、直肠系膜(距肿瘤下缘2 cm)、直肠系膜远端切缘(距肿瘤下缘5 cm)取病理标本,采用免疫组化SP法对标本进行CD44v6检测。

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