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PartⅡFeasibility of vitrifying normospermic samples using droplets without cryoprotectans Objective Discuss the feasibility of droplets to vitrify normospermic samples without cryoprotectants. Methods Normal prepared sperm were frozen by conventional freezing protocol and non-cryoprotectant freezing in droplets respectively. Freezing in droplets means freezing sperm in every 10ul drop. After thawing, compared the two groups'sperm mobilities.

第二部分微滴法无保护剂玻璃化冷冻正常精子的可行性探讨目的探讨在不添加冷冻保护剂的情况下微滴法玻璃化冷冻正常精子的可行性方法收集16份正常精液标本,上游处理后分别进行慢速冷冻和微滴法冷冻,微滴法即用拉细巴氏管将标本形成10ul的液滴,直接投入液氮内冻存,复苏后镜检其活动率。

We observed the cytopathic effect in rabbit kidney cells culture to isolate viruses,then typed viruses by monoclonal antibody and flurescence antinody technique. In the 1,016 .scraping specimens from cervicitis, vulvitis, vaginit;s and cervlcal cancer, we found72 cases of HSV infection the positive rate was 7. 08% The highest positive rate was 8.7% that was in cervical cancer.

本文应用细胞培养观察细胞病变的方法来分离病毒,再用单克隆抗体及荧光抗体对病毒进行分型,在西宁地区1016例妇科疾病所取标本中(包括宫颈炎,外阴炎,阴道炎及宫颈癌等),共发现单纯疱疹病毒阳性标本72例,阳性率为7.08%,而宫颈癌中阳性率最高为8.7%。

It was pointed out that the shortcoming and harm present in the traditional process using white arsenic to make animal specimen .

指出了使用砒霜制作动物标本传统工艺的缺点和危害,介绍了应用制革、制裘技术原理制作动物标本的若干技术要点。

All benign gynecologic processes included endometriosis(n=5), adenomyoma(n=3), atypical endometrial hyperp...

子宫内膜癌患者术前(2-3周内手术)血液标本48例,及术后26例(手术后3-4周,22例术后血液标本未采集到)。

Methods 30 cases with HD and 15 cases of nomal colon were studied by immunohistochemical. 5 fresh colon segments of HD were detected by transmission electron microscopy. Results There was no or weak of Cx43 and ICC in the colon wall of aganglionic part of HD.

取30例HD患儿扩张段及狭窄段肠管标本、15例正常结肠组织,行免疫组化检查,并取5例HD患儿新鲜结肠标本在透射电镜下观察。

Results: There was high detectable rate of Salmonella serotype Derby. Salmonella serotype Agona, and Salmonella serotype Anatum, in both kinds of specimens except high detectable rate of Salmonella serotype Typhi in clinical specimens. Group B of Shigella was the most and Group D took second place. While S. flexneri 2a was detected mostly and 2b was the second.

结果:检出的沙门菌血清型中除临床标本中检出的较多的伤寒沙门菌外,德尔卑沙门菌、阿贡纳沙门菌、肠炎沙门菌和鸭沙门菌在两类标本都有较高的检出率;志贺菌都以B群居多,D群次之,B群中以福氏2 a型检出率最高,2b型次之。

The tissue samples of small intestine, liver and blood samples from peripheral and portal vein of 4 macaques of 6-month fetus, 2-day neonate, 45-day neonate and adult were obtained after anesthetization.

通过手术途径获得胚胎6月、新生2 d、新生45 d和成年猕猴的回肠、肝脏、门静脉和外周血等标本,应用放射免疫分析法测定各标本中的VIP含量;通过免疫组化方法观察VIP在肠、肝组织内的分布;利用原位杂交法检测VIP受体1(VIP receptor 1,VIPR1)的表达。

Methods to analysis the change of blood viscidity and coagulation in angiopathy,50 cases of blood sample coming from the patients and 102 cases of blood sample coming from healthy persons were collected, the relevant data was compared by hemorrheology compute analytical instrument.

收集50例下肢血管病患者的血液标本,采用血液流变学电脑分析仪等设备,分析血液粘稠度和凝固性的变化,并与102例健康人的血液标本进行比较。

Methods Origins, courses, branches, distribution and the anastomosis of the posterior antebrachial cutaneous nerve and its nutrient vessels were studied on 24 upper extremities and 5 fresh cadavers using gross anatomical method.

方法使用24侧上肢标本和5具新鲜整尸标本,通过大体解剖、铸型和动脉造影等方法,研究前臂后皮神经及其营养血管的起始、走行、分支、分布及彼此间的吻合规律。

Methods In 18 adult upper extremity cadavers,origins,courses, branches,distribution and the anastomosis of the medial antebrachial cutaneous nerve,basilic vein and their nutrient vessels were studied under the operating microscope.5 fresh adult cadavers,photographic developer were injected in the artery.

方法上肢标本18侧,显微剥离表皮组织,观察前臂内侧皮神经、贵要静脉及各自营养血管的起始、走行、分支、分布及与其它血管的吻合情况;新鲜整尸标本5具,动脉灌注显影剂,剥离体被组织于X光下摄影,分析皮动脉的分布及其与毗邻血管的关系。

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