标准测定

Result］ The correlation coefficient of standard curve by atomic absorption spectrometry was 0.997 4-0.999 5, the relative standard deviation was 0.36%-2.80% and the recovery was

结果］ 原子吸收光谱法测定的标准曲线相关系数为0.997 4~0.999 5，相对标准偏差为0.36%~2.80%，加标回收率为

Research in quality standard for Rhizoma Drynariae with salt Moisture, heave metal limit examine, arsenic salt examine and animalcule limit examine of ten batch magnifying processing sample are examined, then constitute the quality standard of rhizoma drynariae with salt.

三、盐制骨碎补质量标准研究通过对骨碎补十批中试样品的水分、总灰分及酸不溶性灰分的测定以及重金属限量检查、砷盐检查、微生物限度检查等，初步制定了盐制骨碎补的质量标准。

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

The recovery rate of added water samples was from 85% to 115%. The range of the lowest detection limit was 0.08-0.21 μg/L. The correlation coefficient was more than 0.999 4. The relative standard deviations (n=6) were generally lower than 8.0% for 2 μg/L standard solution of benzene series through 6 repetitions of determination.

结果表明，水样的加标回收率在85%～115%，最低检测限在0.08～0.21 μg/L，相关系数大于0.999 4，浓度2 μg/L的苯系物标准溶液经重复6次测定，其相对标准偏差基本小于8.0%。

Methods: 1. To detect the GC level in the serum by enzyme immunoassay kit.The serum samples were obtained from 22 psoriatic patients, 15 patients who had been clinical cured and 20 normal controls. There were standard, control, and sample wells. The standardN control and serum samples were pipeted into the appropriate wells and then the enzyme conjugate solution, GC antiserum were pipeted into each wells, incubated the wells at room temperature for 45 minutes, washed and dried each well, added TMB chromogen solution, incubated the wells for 15 minutes, added H2SO4 to stop the reaction.

1。酶免疫法测定银屑病患者治疗前、后血清GC水平的变化：血清标本来自22例治疗前银屑病患者、15例临床治愈的银屑病患者第四军匝大学硕＿］，学位论文和 20名正常人对照，设置标准品孔、对照品孔和样本孔，标准品、对照品和血清样品分别加入对应各孔中，在各孔中再加入酶联GC，兔抗GC血清，室温下放置 45分钟后清洗吸干，加 TMP色原，15分钟后 11p。

Methods Conjugated with protein precipitation with trichloroacetic acid, BCA method was used. According to the positive relationship of protein content with the 570 nm absorbance, protein content was calculated in powdered milk.

使用三氯乙酸沉淀蛋白质后，运用BCA法，在570nm波长处，分别测定标准蛋白质应用液与样品稀释液的吸光度值，基于测定液中蛋白质含量与其吸光度值呈正比关系，计算出样品中蛋白质的含量。

Objective To introduce a new method for determination of urinary iodine by using ammonium persulfate digestion, this method was modified from the national standard method of chloric acid digestion.

目的 介绍修订后的尿碘测定标准新方法，即尿中碘的过硫酸铵消化。砷铈(As3+、Ce4+)催化分光光度测定方法，建议各级实验室应尽快应用。

Abstract］ objective: to investigate factors in water-dilution affecting the determination results with biochemical oxygen demand （bod5） standard dilution method, so as to improve the sensitivity and accuracy, and to provide rational suggestion for further study.

目的：旨在寻找生化需氧量标准稀释法测定中稀释水的一些影响因素，提高测定的灵敏度与准确度，为今后的教学研究提供一定的参考。

Abstract］ Objective: To investigate factors in water-dilution affecting the determination results with biochemical oxygen demand （BOD5） standard dilution method, so as to improve the sensitivity and accuracy, and to provide rational suggestion for further study.

作者：张震，金庭旭，张华，方世杰目的：旨在寻找生化需氧量标准稀释法测定中稀释水的一些影响因素，提高测定的灵敏度与准确度，为今后的教学研究提供一定的参考。

These methods oftenyield widely different results so that the results must be traceable to the referencemethod to assure the accuracy and comparability.In 1964 the Expert Panel on Haemoglobinometry of ICSH drew uprecommendations for haemoglobinometry in human blood that were published in1967. The method had been revised three times; the apparatus, reagent and procedurehad been changed. We have established the reference method in our laboratoryaccording to the requirements of ICSH. Its methodology characteristics need to bevalidated whether the method is fit for charaterisation of haemiglobincyanidereference material.

国际血液学标准化委员会于1967年确定了血红蛋白的参考方法，至今该方法已修订三次，仪器、试剂、操作程序等都有改变，本实验室按照ICSH的要求建立了血红蛋白测定的参考方法，为了验证血红蛋白参考方法用于标准物质定值的可行性，我们对血红蛋白测定参考方法的性能进行了评价。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。