标上

Unlatching the lid and opening the box, Langdon turned his attention not to the lettered dials of the cryptex, but rather to the tiny hole on the underside of the box lid.

兰登揭开盖子，把盒子打开，他没把注意力放到密码盒的标有字母的刻度盘上，而是集中到盒盖下侧的小洞上。

By locking laser frequency to atomic absorption spectrum through current negative feedback and locking microwave frequency to electromagnetic induced transparency resonance peak through voltage negative feedback, it is realized to lock the clock frequency of this laboratorial desktop experimental CPT frequency standard.

通过采用电流负反馈将激光频率锁定在原子对激光的吸收峰上，电压负反馈将微波频率锁定在电磁感应透明共振峰上，用该物理系统实现了闭环锁定的实验室桌面CPT频标实验系统。

By locking laser frequency to atomic absorption spectrum through current negative feedback and locking microwave frequency to electromagnetic induced transparency resonance peak through voltage negative feedback respectively, the locking of the clock frequency of this laboratorial desktop experimental CPT frequency standard is realized.

介绍了相干布居囚禁原子频标的小型、低功耗物理系统，并应用它开展实现了CPT原子频标的激光锁定、微波锁定方案的实验室研究。通过采用电流负反馈将激光频率锁定在原子对激光的吸收峰上，电压负反馈将微波频率锁定在电磁感应透明共振峰上，用该物理系统实现了闭环锁定的实验室桌面CPT频标实验系统。

The synthetic procedure consists of three steps: the preparation of magnetic silica microbeads by polymer templating method; coupling of iminodiacetic acid with a bifunctional silane agent 3-glycidoxypro-pyltrimethoxysilane; charging the magnetic support with Cu(superscript 2+) With bovine serum albumin as a model compound, the adsorption behavior of protein on the magnetic matrix was studied.

采用模板法制备的单分散磁性硅胶微球，经过表面修饰偶联上亚氨基二乙酸，与过渡金属离子Cu(上标 2+)螯合，制成一种新型的磁性固定化金属亲和纯化载体。

The observation equation built in this paper is nonlinear. After linea-rization, it can be seen that, the error of the angular sensor on ESG's outer gimbal axis is equal to the azimuth error of ESG, while that on ESG's inner gimbal is coupled from the elevation and the azimuth errors of ESG.

本论文所建立的量测方程是非线性的，其在标称状态的线性化方程表明：静电陀螺外框架轴上的角度传感器的输出误差等于陀螺主轴的方位角误差，内框架轴上的角度传感器的输出误差包括陀螺主轴的高度角误差和方位角误差的耦合量，其中方位角误差的耦合分量是由复示水平基准误差引起的。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Our company newly develop automatic RMXP series bottle-washing units which based on the original domestic technology, aims at the existing fast bottle label, dirt recycling bottle and can't wash clean by normal bottle-washing machine. It can solve the cleansing recovery bottle problem fundamentally of present domestic breweries, milk factory, seasoning factory, fruit juices, beverage plants and various kinds of brewery.

本公司最新研制的RMXP系列自动洗瓶机组，在原有国内产品技术的基础上，针对现有酒瓶贴标较牢、回收瓶较脏，一般洗瓶机不易洗干净而开发研制的装置，其可以从根本上解决目前国内啤酒厂、黄酒厂、牛奶厂、调味品厂、饮料厂以及各类酒厂清洗回收脏瓶的难题。

THE term "bargain basement" was supposedly coined to describe the first outlet of Filene's Basement, an American discount shopping chain whose bags scream "I just got a bargain!" in large red letters. Those bags celebrate a feeling most shoppers will be familiar with: the pleasure of having bought something for less than they were willing to pay. This difference between the price paid and the most a buyer would have agreed to shell out is known as "consumer surplus" and is the part of the overall economic benefit from any trade that the buyer gets.

长期的廉价部被描述成Filene's Basement公司的唯一出路，这是一个用红色字体将&我得到了廉价货&标在袋子上的美国廉价购物链，这些袋子给大多数人一种熟悉的感觉——买到的东西的价格低于预想的价格那种快乐，这种区别在消费的价格和消费者最乐意公开的消费盈余上，它是整个经济从消费者各种交易受益的一部分。

Transport stream decoding is SDTV and HDTV source decoding software. It can decode the transport stream files in hard disk into DTV signal then broadcast, fast forward, fast rewind or pause them on screen.

传输流软件解码是一套标清和高清信源解码软件，能将硬盘上的传输流文件解码为数字电视信号，并可以在屏幕上对其进行快进、快退、暂停、播放。

Mark the fix position of the hinge on the door pocket.

把门扇临时固定在对应的安装位置上，并调整至准确位置，在门套上标出铰链固定位置

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。