柱体

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The influence of polar modifier and column temperature on enantioseparation of benzoin on Chiralcel OD-RH systematically was also studied in the thesis.

另外，安息香对映体在Chiralcel OD-RH柱上的色谱拆分保留机理，特别是极性有机溶剂及柱温度对安息香拆分的影响也是本论文的研究内容。

The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

An interbedded sliding zone with fault-slip cleavage in an amygdaloidal basalt thin layer made up the embryo of the main sliding zone and the coupling chemical weathering with physical weathering based on lateral unloading made the interbedded sliding zone get a material prone to slide finally.

褶皱运动产生的构造裂隙与柱状节理的叠加使滑体玄武岩呈现碎裂—镶嵌结构，而沿结构面发生的化学风化形成的腐岩壳使岩体进一步转变为&石夹土&结构，加剧了坡体的时效变形。

We have incorporated a hydraulically actuated lashing device that either lashes the inner of the lifter to the outer body or detaches the body from the internals of the lifter,thereby not translating the motion from the cam onto the pushrod,"said Nandu Srinivasan,Manager of Advanced Engineering for Engine Air Management Operations at Eaton Corp."

我们已经合并了这种液压驱动装置，即可以使挺柱内部零件和外体紧固，也可以使外体和内部零件分开，当松开时就不会将运动从凸轮传递给出推杆，Nandu Srinivasan，伊顿公司的发动机进气管理工程部门的经理说：&当推杆上受到液压时，它会分开外体，由于没有互相锁定，推杆基本上是在凸轮上，上升下降，就像伸缩望远镜杆一样。&

Furthermore, large-scale fold and fault structure hasn't been found around the Tianchi Lake, but the original prismatic joints and radial joints developed well in the trachyte, so the rock mass is dissected into fractured fragmental structure and the integration of rock mass is further destroyed.

在构造方面，长白山天池周围虽然没有发现大规模的褶皱断裂构造，但粗面岩中原生柱状节理、放射状节理十分发育，这些节理裂隙将岩体切割成十分破碎的碎裂结构，使岩体极易产生岩崩、落石，使岩体的完整性遭到了进一步的破坏。

Hypothesizing that the stress distributions don＇t refer to stressstrain history of explosive in pressing process ,the stress in the condition of steady load for charge of projectile body is analyzed and calculated by two methods: one is that the whole projectile body is divided into smaller lattices to solve ,the other one is that the whole projectile body is divided into smaller lattices firstly, and then local lattices are bridged according to the need for solving.

假设药柱的应力分布不涉及压制过程炸药的应力一应变历史，采用两种方法对装药弹体在静载荷下的应力进行分析计算，一种是将整个弹体划分成较小的网格进行求解，另一种是先对整个弹体进行网格划分，然后根据求解的需要对局部网格进行加密。

We selected different eluents according to different diasteroisomers and gained the singles of the diasteroisomers by the column chromatography.

经过简单易行的薄层色谱分析法选择了不同的洗脱剂，然后通过柱色谱分离，得到了多对非对映异构体的α-体和β-体。

The fluid end of the plunger pump was analyzed with three-dimensional finite element method,and the distribution law of stress at the fluid end was obtained.

对某柱塞泵泵头体进行了三维有限元分析，获得了泵头体的应力分布规律，并应用ANSYS软件对泵头体的结构进行了优化，为类似的壳体零件的优化设计提供了依据。

Torsion would be produced when curve beam under self-weight load, and, torsion and radial force would be produced when structure under prestress load. Such forces would brought prominent side reactions.

在施工阶段和运营阶段，就曾发生过梁体侧向倾覆、支座脱空，墩柱开裂、梁体体侧移以及腹板侧向崩裂等曲线梁结构侧向效应病害，这是技术人员对曲线桥特性把握不够造成的。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。