柰酚

Objective: To estabilish an RP-HPLC method for the determination of kaempferol, naringenin and quercetin in Euonymus alatus Thunb.

目的：建立同时测定鬼箭羽中降糖活性成分山柰酚、柚皮素及槲皮素含量测定的方法。

Their structures were identified by physico-chemical properties and spectral analysis. RESULTS Seven compounds were isolated as kaempferol,quercetin,astragalin,isoquercitrin,kaempferol-3-O-β-D-glucuronic acid methyl ester,4-hydroxybenzonic acid,hentriacontane.

结果分离得到7个已知化合物，分别为山柰酚、槲皮素、山柰酚-3-O-β-D-吡喃葡萄糖苷、槲皮素-3-O-β-D-吡喃葡萄糖苷、山柰酚-3-O-β-D-吡喃葡萄糖醛酸甲酯、对羟基苯甲酸、三十一烷。

A high-performance liquid chromatographic method for the simultaneous separation and determination of aryltetralin lignans and flavonoids in Podophyllum emodi Wall.var. chinesis Sprague was developed. The separation was performed on a Kromasil-C 18 column by gradient elution with phosphate buffer-methanol (0-13min, 50∶50, 33min, 30∶70) as mobile phase at a flow-rate of 0.8 ml/min, with detection 270nm.

首次建立了桃儿七中苦鬼臼葡萄糖甙、4-去甲基鬼臼脂素、表鬼臼脂素、苦鬼臼脂素、苦鬼臼酯酮、鬼臼脂素、鬼臼酯酮、去氧鬼臼脂素、去氢鬼臼脂素等9种木脂素以及山柰酚、槲皮素等2种黄酮类组分同时分离测定的高效液相方法。

Results Eleven compounds were isolated and identified as:quercetin(1),kaempferol(2),quercitrin(3),protocatechuic acid(4),gallic acid(5),protocatechuic acid ethyl ester(6),ethyl gallate(7),vanillic acid(8),1,5,7-trihydroxyl-3methylan...

结果得到11个化合物，分别鉴定为：槲皮素(1)、山柰酚(2)、槲皮苷(3)、原儿茶酸(4)、没食子酸(5)、原儿茶酸乙酯(6)、没食子酸乙酯(7)、香草酸(8)、1，5，7-三羟基-3-甲基蒽醌(9)、琥珀酸(10)、5-羟甲基糠醛(11)。结论化合物6、7、9、11为首次从该属植物中分离得到。

Three flavonoids compounds: kaempferol *!, quercetin *, and isoquercitrin *# were isolated from the leaves of Cyclocarya paliurus Batal.

采用溶剂萃取及多种色谱技术分离纯化，根据理化性质和光谱分析方法鉴定其结构，确定从青钱柳中分离得到山柰酚、槲皮素和异槲皮苷 3个黄酮单体化合物，并用反相高效液相色谱法对其含量进行了测定，测得各自在甲醇和水提取物中的含量：山柰酚 0 。

The theoretical plate numbers of kaempferol and quercetin were 5 121 and 6 673, the resolution between kaempferol, quercetin and impurities were 1.96 and 5.32, respectively.

该方法精密度、重现性和分离效果好，分析快速准确，适合于该药材及提取物中山柰酚和槲皮素含量的测定，不同产地两成分均存在一定的差别。

RESULTS Kaempferol, quercetin and impurities were separated.

结果 槲皮素，山柰酚与相邻杂质能很好分离，其分离度分别为1.96和5.32，理论塔板数以槲皮素峰和山柰酚峰计算，分别为5121和6673；在5～100mg·L^-1内峰面积与其浓度均呈良好的线性关系（r=0.9994，0.9996），样品回收率分别为97.58％和105.67％；两成分含量以湖南张家界的最高而湖北赤壁最低。

Kae is effective in inhibiting the action of PAF and it is a new PAF receptor antagonist.

山柰酚具抗PAF的作用，为一新的PAF受体拮抗剂。

Results showed that hypochromism and red shifted KAE absorption spectra were observed in the presence of DNA,and that synchronous fluorescence of DNA-NR was quenched with addition of KAE,indicating that a strong competition for DNA binding ...

结果表明：加入DNA后，山柰酚的吸收光谱呈现减色和红移现象；山柰酚的加入使得DNA-NR同步荧光强度猝灭，表明山柰酚对NR与DNA的结合为竞争性抑制；山柰酚的加入使得DNA的粘度增大，热变性温度升高。

Results showed that hypochromism and red, shifted KAE absorption spectra were observed in the presence of DNA, and that synchronous、 fluorescence of DNA-NR was quenched with addition of KAE, indicating that a strong competition for DNA binding between KAE and NR existed, in addition, the relative viscosity and the value of melting temperature of DNA increased in the presence of KAE.

结果表明：加入DNA后，山柰酚的吸收光谱呈现减色和红移现象；山柰酚的加入使得DNA-NR同步荧光强度猝灭，表明山票酚对NR与DNA的结合为竞争性抑制；山柰酚的加入使得DNA的粘度增大，热变性温度升高。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。