柚皮苷

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

The proposed method surpassed other chromatographic alternatives in terms of limit of detection at least 10~3 folds with good reproducebility. Chapter 4 A capillary zone electrophoresis with indirect LIF detection method is developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside).

第四章建立了一种毛细管区带电泳—间接激光诱导荧光检测法同时测定6种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的新方法，扩大了LIF检测的应用范围。

METHODS The contents of synephrine,neohesperidin,hesperidin, naringin and narirutin in the raw materials and the extracts of Citrus aurantium were determined by reversed-phase high performance liquid chromatography with an Inertsil ODS-3(4.6 mm×250 mm,5 μm) column, respectively.

方法采用高效液相色谱法，以InertsilODS-3(4.6mm×250mm，5μm)色谱柱分别对枳实及其提取物中辛弗林生物碱类成分和新橙皮苷、橙皮苷、柚皮苷、芸香柚皮苷黄酮类成分进行测定。

Among these high value food ingredients citrus bioflavonoids, formerly known as "vitamin P"(hesperidin, naringin, diosmin, neoeriocitrin, and their respective aglycones, tangeretin, nobiletin, apigenin, luteolin or rutin, among others) have undergone increasing importance.

在这些高价值的食品配料中的柑橘生物类黄酮，以前称为&维生素 P &（橙皮苷，柚皮苷，地奥司明，新圣草苷，以及各自的苷元，桔皮素，川陈皮素，芹菜素，木犀草素或芦丁等）承载了越来越多的重要性。

We can supply monomers, such as Rutin,Baicalin,Amygdalin,Ursolic acid,limonin,?Glycyrrhizic acid, Chlorogenic acid, Paeoniflorin, Gardenoside, Paeonol, Apigenin, Naringin, Naringenin, Astragalus P.E., Soybean Isoflavones, Epimedium Extract and so on.

产品主要有单体（含量90%~98%：苦杏仁苷、柠檬苦素、熊果酸、芍药苷、栀子苷、丹皮酚、芦丁、槲皮素、黄芩苷、黄芩素、芹菜素、绿原酸、甘草酸、柚皮苷、柚皮素；标准提取物：山楂总黄酮、葛根总黄酮、黄芪多糖、枸杞多糖、大豆异黄酮等。

Objective To establish a RP-HPLC method for the determination of naringin and neohesperidin in Fructus Aurantii.

目的 建立枳壳中柚皮苷和新橙皮苷含量测定的方法。

The average recovery of naringin was 101.62%(RSD=1.91%), and that of nephesoeridin was 103.12%(RSD=1.22%).

柚皮苷的平均回收率为101.62%(RSD=1.91%)，新橙皮苷的平均回收率为103.12%(RSD=1.22%)。

RhaG hydrolyzed only the 6-C α-rhamnoside of ginsenoside Re, RhaR is able to hydrolyze the α-rhamnoside of rutin、hesperidin and naringin.

实验结果表明，RhaG只水解人参皂苷Re的C-6位末端上的一个α-鼠李糖基，而RhaR能水解芦丁、橙皮苷、柚皮苷的α-鼠李糖基。

Molecular Formula and Molecular Weight : C27H32O14; 580.53Melting Point and solubility: When crystallized from water ,it contains 6 to 8 mol H2O, mp 83℃. After drying at 110℃ to constant weight, it contains 2 mol H2O, mp 171℃. Sol in methanol,alcohol, acetone, warm acetic acid,dil alkali and hot water.

熔点及溶解度：纯品柚皮甙中结晶水的含量及其熔点因结晶和干燥方法而异，以水作溶剂结晶所得柚皮甙分子中含6～8个结晶水，熔点83℃；而在110℃下干燥至恒重后得到的柚皮苷分子含有2个结晶水，其熔点升至171℃。

Jizhi Syrup,one of well-sold Traditional Chinese Medicine for treating respiratory infection,cough,acute tracheitis and bronch,etc.

标准收载于《中国药典》2000年版和2005年版一部，规定有原儿茶酸、麻黄碱、柚皮苷为对照的定性鉴别和对柚皮苷进行的含量测定。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。