染色质的

Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

光镜下H-E染色切片中腺上皮区仅可以区分出四种类型的细胞：弱嗜碱性纤维样细胞、强嗜碱性纤维样细胞、嗜酸性颗粒分泌细胞和纤毛细胞等；腺上皮下的基底膜区有少量结缔组织存在；扫描电镜下可以观察到鳃下腺表面的纤毛及腺细胞的分泌物等情况；透射电镜下观察到腺上皮中有支持细胞、感觉细胞和7种类型的腺细胞；近基底膜区观察到富含粗面内质网的细胞；基底膜间为薄层疏松结缔组织，内含肌细胞及神经末梢等结构。

Methods: Study the newly found cell with the weak silver carbonate staining method of del Rio-Hortega. Using transmission electron microscopy, compare the newly found cell with the atypical cell found by Marc Lenoir and Philippe Vago, and extend young SD rat model to adult SD rat, young and adult Wistar rat,and extend drug of Neomycin to Amikacin to study the universality.

采用经典的小胶质细胞特殊染色方法——银染法(Hortega氏碳酸银法)对大鼠耳蜗药物损伤后出现的新细胞进行研究；通过透射电镜与Marc Lenoir和Philippe Vago实验动物模型中的"非典型细胞"进行了比较与扩展研究；运用逆转录PCR技术检测耳蜗基底膜上神经干细胞标志物nestin的表达情况，对新细胞的来源及其与神经干细胞或nestin的前体细胞的关系作进一步研究。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology.

背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组，给药组涂抹红鹿制剂，对照组涂抹化妆品基质，连续涂抹60 d；应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量，结果表明，虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin，白色闭壳肌中未检测到类胡萝卜素，其中肝胰腺除上述两种类胡萝卜素外，还含有其他多种未鉴定的类胡萝卜素；在各组织中，雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高；橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝；雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察，并未观察到异常现象；超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌，在橘红色闭壳肌中有三种肌纤维，其中疏松型肌纤维为橘红色闭壳肌特有。

In order to protect the natural materials sourcing, product merchantability Masamori companies have sufficient reserves, the use of production advantages, to supply customers Gannan crafts of all kinds of natural areas, including: moss products, GREEN MOSS, moss garden, moss flower pots, stained moss, moss basket, moss ball, sphagnum ball, jade moss, moss animals, mountain moss, moss, grass, green, Culture Media, rattan, pine, moss, moss, water and agricultural products, and take the finished product semi-finished products processing; Through the joint efforts of Tongren, Masamori companies have formed a certain scale, the scale of business in the continuing development and growth, and the successful completion of a trade company from production-oriented enterprises to transition.

为了保障天然材料的货源，正盛公司对适销产品均有充分的储备，利用产地优势，向客户供应赣南地区各种天然工艺品，包括：青苔制品、GREEN MOSS、青苔园艺、，青苔花盆、染色青苔、青苔吊篮、青苔球、水苔球、青苔玉、青苔动物、山苔、青苔草、绿苔、栽培基质、藤条、松果、苔藓、青苔、水草及农产品，并承接成品、半成品的加工；经过同仁的共同努力，正盛公司已形成一定规模，业务规模在不断的发展与壮大，并且顺利完成了由贸易型公司向生产型企业的过渡。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF. 10 days later after the administration of PLGA microspheres, the effects of stimulus to synthesis of type Ⅱ collagen and proteoglycan can be compared with continue administration of free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短；bFGF缓释微球能通过持续释放活性bFGF，在较长时期内促进软骨细胞的分裂增殖；Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示，应用微球10天后，能有效的促进软骨基质成分的合成，其效果与持续应用游离bFGF的效果相当。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短；bFGF缓释微球能通过持续释放活性bFGF，在较长时期内促进软骨细胞的分裂增殖；Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示，应用微球10天后，能有效的促进软骨基质成分的合成，其效果与持续应用游离bFGF的效果相当。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。