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Results: Neonatal rats in hypoxic-ischemicgroup behaved restlessness, cyanosis, deep and rapid breath, astasia, lethargy, irritation and spasm; There were local pyknosis, fragmentation, dissymmetry, blur or disappearance of nucleoli, raritas of ground substance in left cerebral cortex; but there was no statistic significance in chinese traditional medicine group and sham group. The expression of HIF-1α mRNA in brain tissue of experimental group and chinese traditional medicine group were higher than that in sham group, especially chinese traditional medicine group at 3d; HIF-1α positive cells were found in cerebral cortex and hippocampus. There were more HIF-1α positive cells in experimental group and chinese traditional medicine group and mainly in vascular endothelial cells.

结果 缺氧缺血后大鼠相继表现为烦躁不安、全身发给、呼吸加深加快、站立不稳、嗜睡、激惹或间断发作的痉挛和抽搐;HE染色显示实验组大鼠左侧大脑皮层出现局灶性神经元核固缩,核碎裂,核仁偏位,不清或消失,基质疏松;中药组大鼠脑组织结构基本接近假手术组,无明显水肿和坏死表现;实验组和中药组大鼠脑组织HIF-1α mRNA表达较假手术组明显增加,尤其是中药组实验第3日者;免疫组化显示实验组和中药组各时间点大鼠大脑皮层和海马区均可见不同程度的HIF-1α表达,明显高于假手术组,阳性表达主要在血管内皮细胞,海马和皮层的锥体细胞亦有HIF-1α阳性细胞分布。

A trichrome stain highlights the collagenous connective tissue of pulmonary fibrosis in blue.

这是肺间质纤维化的三色染色的镜下图片,可见肺组织的纤维化区被染成蓝色。

Staining and observing shows that the most pathological changes were interstitial pneumonia and hemorrhagic pneumonia, typical nonsuppurative encephalitis, and neurogangliitis et al. The lymphoid tissue was necrosis, and the amount of lymphocytes was reduced. The pathological changes of the positive cells, especially the epithelial cells were swell, denaturization and hyperplasy to the extent.

H.E。染色观察显示,病理组织学变化主要表现为间质性肺炎和出血性肺炎、典型的非化脓性脑炎、神经节炎等;淋巴结、扁桃体和脾脏等组织内淋巴细胞坏死、数目减少;有阳性反应的细胞,特别是阳性上皮细胞呈现不同程度的肿胀、变性、增生等病理变化。

Methods:MTT assay was used to detect PGPG homotypic adhesion and PGmatrix adhesion,and PGHUVEC adhesion was determined by Rose Bengal stain.

小檗碱(5、10 μg/ml)处理PG细胞24小时,采用MTT法观察PG细胞与PG细胞的黏附,PG细胞与细胞外基质(FN和Martrigel)的黏附;用虎红染色法观察PG细胞与人脐静脉内皮细胞的黏附。

Compared with control group,the ADM level in the left lung increased(P<0.001),but the ADM levels in the heart,liver,kidney decreased.The ratio of wet to dry weight of the left lung in experiment group was 6.491±0.248,and 5.937±0.361 in control group,there was no significant difference(P>0.05).No alveolar wall thicken,alveolar and mesenchyma edema were found in two groups.

实验组兔左肺的湿/干重量比为6.491±0.248,右肺的湿/干重量比为5.937±0.361,双肺的湿/干重量比差异无显著性(P>0.05);HE染色结果显示实验组与对照组均未见肺泡壁增厚、间质及肺泡水肿等改变。

Results: All the slides of the breast carcinoma in our department were reviewed, 9 cases of glycogenich, clear cell carcinoma, 4 cases of mucinous carcinoma, 2 cases of tubular carcinoma, 18 cases of metaplastic carcinoma, 6 cases of apocrine carcinoma, 1 case of secretary carcinoma, and 2cases of lipid-rich carcinoma were found.

结果:回顾42例乳腺癌病理切片,发现富含糖原的透明细胞癌9例,黏液癌4例,小管癌2例,化生性癌18例,大汗腺样癌6例,分泌型癌1例,富脂质癌2例;不同组织类型的乳腺癌具有不同的光镜、特殊染色及电镜表现。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞,通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色,比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

All 31 YSTs (5 pediatric and 26 postpubertal) showed strong positive SALL4 staining in more than 90% tumor cells but had negative OCT4 staining. Both spermatocytic seminomas showed positive SALL4 staining in 80% to 95% tumor cells in all 3 types of tumor cells with weak-to-moderate staining intensity.

为了检验SALL4的特异性,我们还给23例睾丸的非GCTs(10例间质细胞瘤、4例支持细胞瘤、3例腺瘤样瘤、3例睾丸旁横纹肌肉瘤、2例弥漫性大B细胞瘤、1例睾丸网的乳头状囊腺瘤)和275例非睾丸性肿瘤(158例转移癌、12例转移性黑色素瘤、11例原发性和2例转移性间皮瘤、72例原发性和20例转移性肉瘤)做了SALL4免疫染色。

Results: The cultured chondrocytes were polygonal cells. There were many rough endoplasmic reticula and mitochondria in cytoplasm, and a lot of secretory vesicles under cell membrane and in the cytoplasm. When cultured for 10 days, some small and white nodules were formed on the bottom of the culture dished, and volcanic-mouth-like structures were formed when cultured for 20 days. Both these nodules and structures contained GAG-positive substances were demonstrated by safranine-O staining.

结果:体外培养的软骨细胞为多角形,透射电镜下见胞浆内有丰富的粗面内质网系统及线粒体,胞膜下及胞浆中有较多分泌泡;连续培养10天时,培养瓶底部出现肉眼可见的白色结节,蕃红-O染色显示白色结节含大量氨基葡聚糖阳性物质,20天时形成火山口样结构,也被蕃红-O染成深红色。

In order to remove the impurity accreted on the cotton and flax fiber, such as pectin, lignin and so on, the floret silk/flax and floret silk/cotton fabric were in need of scouring and bleaching, and we use W100 and H_2O_2 to treat them.

为了去除棉、麻纤维上的果胶、术质素和色素等杂质,提高染色的效果,我们采用了精炼剂W100对坯布进行精练,然后用双氧水漂白,收到了较好的效果。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

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