染色质的

Technique details were optimized about steps from sample treatment to gel staining, and 2 - DE gels are lampros and aequalis distributed , the method of sample treatment suitable for mass spectrography analysis.

优化从样品处理到凝胶染色几个过程的技术细节，蛋白表达谱清晰且分布均匀，样品处理方法适合后期的质谱测试分析。

Autoradiography of aortas demonstrated uptake of the agent into macrophage-rich atheromata identified by Oil Red O staining of lipid deposits.

以油红O染色确定脂质沉积处的动脉粥样硬化瘤，放射自显影技术显示该瘤中富含巨噬细胞并摄取显像剂，荧光镜和流式细胞仪显示主动脉的细胞中主要是巨噬细胞摄取显像剂。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变（包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例）组织中β-catenin核阳性的表达，同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

Results By using zinc-iodide-osmium staining, the tubulo-vesicular system of parietal cells of fundic gland was manifested as numerous fibrous structures under HVEM. The nematolysosomes was often demonstrated as branching in shape and anastomosed with each other making a network system in cytoplasm under the visual field of the three dimensional electron microscopy combined with AcPase cytochemistry.

结果高压电镜下显示，锌-碘-锇酸染色方法制备的标本胃底腺壁细胞的微管泡系统呈无数纤维状结构；AcPase结合三维电子显微镜观察显示，线性线粒体在胞质中常呈分支状并互相吻合成网。

What was observed under a light microscope included: tumor cells were mulberry and micropapillaryshaped or it was of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes; immunohistochemical staining showed EMA positive location was both at outward surface of glandule duct and at micropapillarylike cancer nest.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列，癌巢与周围间质形成明显的空隙。微乳头缺乏纤维血管轴心。免疫组化染色EMA阳性部位在癌细胞巢团或微乳头状、腺管的外表面。

What was observed under a light microscope included: tumor cells were mulberry and micropapillary shaped or it was of glandule tubular arrangement; there was obvious interspace between cancer nest and neighboring areas; micropapillary was empty of fiber blood vessel axes; immunohistochemical staining showed ema positive location was both at outward surface of glandule duct and at micropapillary like cancer nest.

光镜下特征性表现为肿瘤细胞呈桑椹状、微乳头状或小腺管样排列，癌巢与周围间质形成明显的空隙。微乳头缺乏纤维血管轴心。免疫组化染色ema阳性部位在癌细胞巢团或微乳头状、腺管的外表面。

The change of α1,2-FT activity in the cell line before and after the tranfection was confirmed by the determination of enzymatic activity. The changes of cell lipid and glucolipid, especially the change of type Ⅱ oligosaccharide, in the cell line before and after the transfection was determined by Thin-Layer Chromatography and TLC immunostaining method, respectively.

通过酶活性测定证明转染前后细胞系α1，2-FT活性的改变，采用薄层层析、薄层层析免疫染色方法测定转染前后细胞脂质及糖脂，特别是Ⅱ型寡糖的变化。

Methods: After transferred pcDNA3.1/hTM plasmid into rabbit artery by high-pressure injection, rabbit common iliac artery were cut and anastomosed again. At the 14 days and 28days after second operation, we checked inside diameter of anastomotic stoma and blood flow velocity by color Doppler. The treated artery were sliced and stained by Verhoeff. Local neointima formation and the ratio stenosis of intervascular were calculated by computer.

用注射式加压转染的方式对兔动脉壁转染pcDNA3.1/hTM质粒，再制造动脉损伤-阻滞模型，于术后14天、28天用彩色多普勒观察活体吻合口内径和血流流速；再做病理切片Verhoeff染色，观察血管内膜增生的程度、部位，计算血管内膜面积、中膜面积和血管狭窄率。

Immunohistochemical staining with an anti-type II collagen antibody demonstrated rich production of the type II collagen in the pericellular matrix from the chondrocytes.

用抗II型胶原抗体免疫组织化学染色证明在软骨细胞周围的基质中有丰富的II型胶原产生。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。