染色质的

Neuronal cell bodies and fibers with moderate mGluR7-LI were seen in the olfactory bulb,anterior olfactory nucleus,piriform cortex,septofimbrial nucleus,bed nucleus of the strial terminalis,lateral hypothalamic area,paraventricular hypothalamic nucleus,supramammillary nucleus,medial and lateral mammillary nuclei,most part of the thalamus,medial and lateral geniculate bodies,nucleus of the optic tract,red nucleus,substantia nigra,interpeduncular nucleus,pontine nuclei,lateral parabrachial nucleus,superior olivary complex,nucleus of the trapezoid body,motor nucleus of the trigeminal nerve,facial nucleus,ambiguus nucleus,cochlear nucleus,vestibular nuclei,nucleus of the solitary tract,hypoglossal nucleus,prepositus hypoglossal nucleus,medullary reticular formation,Purkinje cells of the cerebellum,nucleus raphe obscurus,intermediolateral nucleus of the spinal cord,Onuf′s nucleus and lamina X of the spinal cord.

呈中等强度染色的神经元胞体和纤维见于嗅结节、前嗅核、梨状皮质、隔伞核、终纹床核、下丘脑外侧区、下丘脑室旁核、乳头体上核、乳头体内、外核、丘脑大部分核团、内外侧膝状体、视束核、红核、黑质、脚间核、桥核、臂旁外侧核、上橄榄复合体、斜方体核、三叉神经运动核、面神经核、疑核、耳蜗核、前庭核簇、楔束外核、孤束核、舌下神经核、舌下神经前置核、中缝隐核、延髓网状结构、小脑蒲肯野细胞层、脊髓中间带外侧核、Onuf核和中央管周围灰质。

To understand how to use differential centrifugation to separate organelle and biological molecular;microspectrophotometry;immunofluorescence technifue and immune electron microscopy;in situ hybridization; aser scanning confocal microscopy;the method to show the nucleic acid、protein、enzyme、glucide and lipid; negative staining; freeze etching and quick freeze deepetching; flow cytometry.

了解用超速离心技术分离细胞器与生物大分子；显微分光光度测定技术；免疫荧光技术与免疫电镜技术；原位杂交技术；激光共焦点扫描显微镜技术；细胞内核酸、蛋白质、酶、糖类与脂质等的显示方法；负染色技术冷冻断裂和冷冻蚀刻电镜技术；流式细胞仪。

The expression of MMP-3 was higher than that of MMP-9 in the stroma of the endometriotic tissues.

在异位内膜的间质和腺体，MMP-3染色均较MMP-9增强(P＜0.01 P＜0.05)。

The CCND2 expression cell proliferation, cell cycle and cell apopotosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.

设计、合成CCND2短发夹RNA双链DNA模板，构建表达质粒pGenesil-2／CCND2，转染LP-1细胞，以RT—PCR检测对CCND2表达的抑制作用，以锥虫蓝染色计数和MTI检测细胞增殖，以Annexin V检测细胞凋亡，流式细胞术检测细胞周期。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基－4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。

Rats in the normal group did not receive any intervention.③Proliferating cell nuclear antigen,β-Ⅲ tubulin and glial fibrillary acidic protein expression in ependyma was determined by the immunohistochemical staining and image analysis.

实验评估：采用免疫组织化学染色法和图像分析技术检测增殖细胞核抗原、β－Ⅲ型微管蛋白（Tuj-1）、胶质原纤维酸性蛋白在室管膜下的表达。

Before the experiment and in the second,fourth week of the experiment,blood was taken from mice's epicanthic intravenous of each group to determine biochemistry index of SCr,BUN,blood potassium,blood sodium and radioimmunity index of PCⅢ,HA,LA.

在实验前及实验的第2、4周，分别从各组大鼠眼内眦静脉采血，测定血肌酐、血尿素氮、血钾、血钠等生化指标以及Ⅲ型前胶原、血清透明质酸、层粘连蛋白等放免指标，42d时从心脏直接采血复查上述各指标，而后处死动物取肾脏进行组织形态学及特殊染色检查。

At 5 days, immunocytochemical staining demonstrated positive reactions for glial fibrillary acidic protein and neuron specific enolase.

神经干细胞球诱导5 d，免疫细胞化学染色后可见胶质纤维酸性蛋白及神经元特异性烯醇酶呈阳性表达的细胞。

This is because neuroanl damage, reactive gliosis or loss of GFAP has not yet completed in such a short duration after an ischemic stroke.

因为在数小时内神经细胞死亡及胶质细胞增生之组织型态学以及GFAP 染色丧失或反应性增加之组织化学的变化皆尚未完成。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐