染色质的

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

Callose staining with particular fluorescence dye-Aniline blue indic-ated that the degradation of callose was delayed.

利用胼胝质特异性荧光染料甲基蓝对花药进行染色，表明突变体胼胝质降解延迟，导致小孢子不能从四分体中释放出来，最终没有花粉粒的形成。

The patient has a history of chondrosarcoma and now, this is recurrent/metastatic chondrosarcoma.

本例用这种染色特别容易认识肿瘤产生的基质，本例肿瘤就是富于粘液样软骨基质。

The bulk of cells in the positive control group were dead.2 Treated with different dosages of DMF for 24h, it can be observed that green,nuclear margination and chromatin condensation were distinct in cells exposed to DMF.

研究结果 1。二甲基甲酰胺致人肝细胞凋亡形态学观察： 1不同剂量DMF染毒12h后，AO／EB染色可见阴性对照组无明显凋亡细胞，50mmol／L剂量组开始出现细胞形态接近正常，胞质为亮绿色，核出现固缩呈圆珠状，与正常细胞比较核质比变大的细胞。

RESULTS: Levels of insulin, FFA, GOT and GPT in serum were significantly increased in model group; activities of T-SOD and GSH-Px in liver tissue and ISI were notably decreased, and content of lipid perhydride MDA was increased. HE staining revealed that there was hepatic cellular swelling in hepatic lobules, inequality of size of lipid droplets in periplast, nucleus on one side, narrowed sinus hepaticus and inflammatory cell infiltration in hepatic lobules and portal area.

结果： 模型组大鼠血清胰岛素、FFA、GOT和GPT水平明显升高，肝组织中T-SOD和GSH-Px活力和ISI明显下降，脂质过氧化物MDA含量显著增加， HE染色示肝小叶中大部分肝细胞肿胀，胞质中出现大小不等的脂滴空泡，以大泡性脂肪滴为主，核居边，细胞界限不清，肝窦狭窄，且存在小叶内及门管区炎症细胞浸润。

Using GSA-〓 lectin histochemistry, the first appearance of microglia occurred at E13, usually stained as scattered amoeboid cells. From then on, the number of microglia increased steadily, reached a peak at postnatal day 7 (P7), and then reduced gradually to adult level. Generally the morphological form of microglia undergoes transformation from early rounded or ameboid to adult resting ramified as they differentiate during development.

使用凝集素GSA-〓亲和组织化学染色，本实验最早观察到小胶质细胞在胚胎13天（E13），为散在分布的圆形或阿米巴样细胞，随后细胞数量逐渐增多；总体而言小胶质细胞的形态遵循着由圆形或阿米巴样逐渐转向纤细分枝状的演变规律，不同阶段细胞形态各有其特点，在不同区域也存在差异；小胶质细胞的数量在生后第七天（P7）达到高峰，其后开始逐步降低直至成年。

Using cytohischemical staining methods, with the results of comparison the dynamics of proteins and polysaccharides in the anthe wall cells and colule cells of different developmental phases in female and male flowers, the anormogenesis of anther tapetum in the inflecting phase of from bisexual flower to unisexual flower was observed. In microspore developed phase, the tapetum functions of preserving and transportingmutritive material for microspore development and of secreting callosal enzyme for decompositing callosal cell lost no normal guadrant formed, of being abnormal meiose of pollen mother cells, and then, the stamen aborted selectively in female flowers and pistil in male flower.

利用细胞组织化学染色技术，对雌、雄花雄蕊花药壁细胞及花药内细胞发育过程中多糖及蛋白质动态进行了比较，实验中观察到雌花在从两性至单性花转变时期雄蕊绒毡层在整个发育过程中表现异常，在小孢子发育过程中未能起到贮藏、转运营养物质供小孢子发育及适时分泌胼胝质酶溶解胼胝质壁的功能，并且花粉母细胞减数分裂异常而未形成四分体结构，进而导致雌花雄蕊选择性败育，而雄花中雌蕊组织也发生了选择性败育过程。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

All rats were sacrificed at 12 weeks after operation. the macro-pathological changes of samples were graded by mankin's scale.bone mineral density of right distal femoras and femora condyles were measured by dual-energy x-ray absorptiometry scanner.

术后12周处死动物取材，采用mankin评分系统评分；用双能骨密度仪测量右侧股骨远端1/4骨密度和股骨内外侧髁软骨下骨的骨密度；右侧股骨髁脱钙后切片，行番红&o&染色和mmp-13免疫组化染色；右侧胫骨近端做硬组织切片，测量软骨下松质骨的骨组织形态计量学参数。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用：主要表现为：(1)对受损神经元具有直接的保护作用，可提高受损神经元的活性和存活率，降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量；(2)对受损的星形胶质细胞也有直接的保护作用，可提高其活性、存活率以及培养液蛋白质的含量；(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力；(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达，降低受损神经元对bax和caspase-3的表达；(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元，因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用，统计学上具有显著性差异。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。