染色质的

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Methods A lamellar flap was produced in the rabbit cornea with a microkeratome. The flap interface in whole corneas and corneal beds were soaked in the rAAV –EGFP solution for 10 min, BSS solution was applied in the control group. The corneas were harvested at day 1, 3, 14, and 21 after application for the structure analysis of cornea by HE staining. Expression of the transferred gene was monitored by fluorescence stereoscope and laser confocal microscope. Results After 3d of infection with rAAV-EGFP ,slight fluorescence was detected in rabbit cornea by fluorescence stereoscope .

微型角膜刀制作兔角膜基质瓣；转染组用含rAAV－EGFP转染液浸泡角膜瓣和角膜基质床10 min，对照组用等量BSS液浸泡角膜瓣和角膜基质床10 min，不同时间点(1、3、7、14、21d)通过荧光体视镜观察角膜出现EGFP荧光的起始时间及分布情况，收集角膜，其中一半用激光共聚焦显微镜观察、照相，另一半固定后行HE染色。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

Methods HE and immunohistochemistry were used to observe the changes of glial fibrillary acidic protein and CD34 in the surrounding areas after cerebral infarction in human.

方法采用HE染色观察星形胶质细胞和微血管的变化，免疫组化技术检测胶质纤维酸性蛋白和CD34在星形胶质细胞和微血管的表达。

Following PAS staining, glycogen in hepatoma carcinoma cells mainly expressed near to unilateral cytoplasm surrounding nuclei, showing strong positive expression in the positive control group. Big karyoplasmic ratio and weak expression of glycogen in cytoplasm were visible in the embryonic hepatic progenitor cell group.

PAS染色后阳性对照组肝癌细胞的糖原主要表达在靠近一侧的核周边胞质内，呈强阳性；而胚胎肝前体细胞诱导组，核浆比例大，部分细胞胞质内可见较弱的糖原表达，充满胞质。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

In normal group, the staining of FN formed intact linear structure at basement membrane and presented regular striae form in connective tissue.

正常组织基底膜中FN染色呈完整线性结构，结缔组织间质中FN染色呈规则的条纹状。

By means of experiments of marker transfer,acridine orange sensitivity test,F' curring and mini-chromosome transformation it is.conchi-ded that the F' plasmid is always in an integrated state in the Sin strains and that the initiation of the replication of the bacterial chromosome is carrie...

标记转移、吖啶橙敏感性，F′质粒消除和mini-染色体质粒转化等实验说明，Sin菌株中F′质粒始终处于整合状态，并且在40℃中细菌染色体的复制由整合状态的F′质粒所带动。

By means of experim ents of marker transfer,acridine orange sensitivity test,F' curring and mini-chr omosome transformation it is concluded that the F' plasmid is always in an integ rated state in the SIn strains and that the initiation of the replication of the bacterial chromosome is carried on by the integrated F' plasmid.

标记转移、吖啶橙敏感性、F′质粒消除和mini-染色体质粒转化等实验说明，Sin菌株中F′质粒始终处于整合状态，并且在40℃中细菌染色体的复制由整合状态的F′质粒所带动。

Results 1、 Generally, we can see the original blue and white, shiny, no cracks in the articular surface of the cartilage after the stress increases gradually yellow, surface roughness, cracks appear; when the pressure decreases, the yellowing, rough, the color of the fracture restore gradually and become shiny.2、the shiny smooth surface can be seen under a light microscope, formation, cell distribution, tidy, clear the level of cartilage at the articular surface stress increases, the surface roughness changes, defects, disordered cells, uneven dyeing ; when the articular surface of the pressure gradually decreased, the cartilage gradually repair and the surface of cells at the surface appear only disorder.3、immunohistochemical observation can be seen throughout the observation period, cartilage cells are type Ⅱ collagen expression and expression after 3 weeks gradually weakening, when the seventh week begin to strong gradually.4、 electron microscopy shows that when stress increases the articular surface, the cartilage cells became flat, the cytoplasm in the endoplasmic reticulum, Golgi apparatus decreased with collagen disorders; and when stress decreases the articular surface, cartilage cells gradually returned normal, cytoplasm in the endoplasmic reticulum, Golgi body gradually restore quantity; collagen fibers with a gradual rules.

结果：①大体观察可见到原本蓝白色、有光泽、无裂纹的软骨在关节面压力增大后，逐渐呈灰黄色，表面粗糙，出现裂隙；当压力逐渐减小后，变黄、粗糙、有裂隙的软骨颜色逐渐恢复，变得有光泽②光镜下可见表面光滑、平整，细胞分布均匀、整齐，层次清楚的软骨在关节面压力增大后，表面变粗糙、缺损，细胞排列紊乱、染色不均；当关节面压力逐渐减小后，软骨表面逐渐修复，细胞仅在表层排列紊乱③免疫组织化学观察可见整个观察期内软骨细胞胞浆内均有Ⅱ型胶原表达，术后3周内表达逐渐变弱，从第7周时开始逐渐变强。④电镜下可见当关节面压力增大后，软骨细胞逐渐变扁，胞质中内质网膜、高尔基体减少，胶原排列紊乱；当关节面压力减小，软骨细胞形态逐渐恢复正常，胞质中内质网膜、高尔基体数量逐渐恢复；胶原纤维排列逐渐有规则。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。