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Recently, a variety of regulatory proteins including DNA methyltransferases, methyl-CpG binding proteins, histone-modifying enzymes, chromatin remodeling factors, and their multimolecular complexes have been identified.

最近,包括DNA转甲基酶,CpG甲基化结合蛋白质,组蛋白修饰酶,染色质重构因子,和它们的多分子复合物等各种调控蛋白质被识别。

All cells were generally smaller and occasionally spindled, the chromatin detail was attenuated, and nucleoli were more prominent.

所有的细胞都稍小,偶见梭形,染色质不清,核仁突出。

A few of intercellular electron-dense zones without typical synapse were found in the inner plexiform layer.

内核层内可见胞核各异的多种细胞,部分胞核常/异染色质例接近,核仁偏心分布。

INI1 (hSNF5/SMARCB1), a member of the SWI/SNF chromatin remodeling complex located on chromosome 22q11.2, is deleted or/or mutated in strictly defined malignant rhabdoid tumors of infancy.

INI1 (hSNF5/SMARCB1)是SWI/SNF染色质重塑复合体(位于染色体22q11.2)中的一员。

INTRODUCTION All DNA-dependent processes replication, tran- scription, recombination, etc.

导言 所有DNA依赖进程(复制,转录描述,重组等)中的真核细胞发生在染色质模板,其结构单元是一个nucleosom

Furthermore, flow cytometry showed that the number of cells in G1 phase increased and the number of cells in S phase decreased, the number of cells in G2/M phases relatively increased. The changes of subcell structure could be seen, such as cavernous cells, cytoplasm agglutination, increasing apoptosis.

结果:0、10、20和40mgL^(-1),姜黄素对Hela细胞增殖的抑制率分别为3.0%、21.4%、32.8%和49.2%,且呈剂量依赖性;流式细胞术显示,G1期细胞增多,S期细胞减少,G2/M期细胞相对增多;细胞结构显示,细胞空化、染色质凝集、凋亡小体增多。

Results Curcuma (0,10,20 and 40 mg·L-1)had obvious inhibitory effects on the Hela cell proliferation in a dose-dependant manner,the inhibitory rates were 3.0%,21.4%,32.8% and 49.2%,respectively. Furthermore, flow cytometry showed that the number of cells in G1 phase increased and the number of cells in S phase decreased, the number of cells in G2/M phases relatively increased. The changes of subcell structure could be seen, such as cavernous cells, cytoplasm agglutination, increasing apoptosis.

结果:0、10、20和40 mg·L-1姜黄素对Hela细胞增殖的抑制率分别为3.0%、21.4%、32.8%和49.2%,且呈剂量依赖性;流式细胞术显示,G1期细胞增多,S期细胞减少,G 2/M期细胞相对增多;细胞结构显示,细胞空化、染色质凝集、凋亡小体增多。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷(5-Aza-2'-dc,以下简称Aza)及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后,用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况,用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况,并用逆转录聚合酶链反应检测ING1bmRNA表达。

The cleaved form of SSH3 translocated from cytosol to nuclear and bound to chromatin.

在细胞凋亡过程中,这种剪切形式的SSH3可以从细胞质转移到细胞核,并结合到染色质。

Sequential chromatin immunoprecipitation assays indicate that H3K4m3 and H3K27m3 co-occupy a fraction of nucleosomes on some but not all lineage-specific promoters examined.

连续染色质免疫沉淀分析表明,在所检测的部分而非所有谱系特异性启动子,H3K4m3和H3K27m3共定位于核小体。

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