染色质的

Results: electronic microscope showed that in prednisone group heterochromatin increased in some cells, entoblast was not obvious in some cells, fat drops were small and few, mitochondria vacuole were common, and myelin figure was found in smooth endoplamic reticulum. the ultrastructure in yc group was almost normal and megamitochondrion formation was common. a large amount of fat drops were found in cytoplasm in gw group and little improvement was found compared with that in prednisone group.

结果： 电镜结果显示：激素组个别细胞异染色质增多，核仁不明显，脂滴显著变小变少，线粒体空泡化常见，有滑面内质网髓鞘样结构形成；养阴组电镜结构基本正常，其特征是细胞内有较多的巨大线粒体形成；桂附组较激素组改善不明显，其特征是胞质内可见大量脂滴。

And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色，0.5mMSNP单独处理的细胞大部分核被染色，而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征；提取细胞DNA电泳分析，观察到了DNA LADDER，这说明，该细胞可能已经发生凋亡，而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数按摩明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Observation on the ultrastructure of the radicle cell of cabbage seeds indicated that the ultrastructure changed with the seed vigor reduced, such as the segregation of cytoplasm from the cell wall, the fragmentation of cell membrane, the vesiculation of protoplasm, the homogenization of chromation, the disintegration of cytoplasm, the distinguishing of nuclear membrane and nucleous ,and so on.

胚根细胞亚显微结构电镜观察结果，甘蓝种子通过人工老化活力降低后，胚根超微结构发生了变化，主要表现为：质壁分裂，细胞膜内陷或局部断裂，细胞质空泡化，细胞器难以辨认，染色质均质化，核仁和核膜的界限模糊等现象。

Results In the heated bone ,bone lacunae were empty ,no bone cells existed ,bone lamella was seen ,granular basophilia staining was seen in some parts of the lamella. In bone medulla , no cell existed , thrombosis occurred in some blood ,partial bone lamella disappeared.

结果 整个视野内骨陷窝空虚，无骨细胞存在，骨板尚清晰，部分骨板出现颗粒状嗜碱性染色，骨髓腔内，基本无细胞结构，血管闭塞，哈佛氏系统结构不清，部分区域骨板层状结构消失，呈一片均质的嗜伊红染色。

One of the serially aligned beadlike granules of concentrated ''.'chromatin'.

染色粒在细胞分裂早期，一条顺序排列的珠状染色质颗粒链构成了染色体

One of the serially aligned beadlike granules of concentrated chromatin that constitutes a chromosome during the early phases of cell division.

染色粒在细胞分裂早期，一条顺序排列的珠状染色质颗粒链构成了染色体

Electronic microscopic observations showed that the swollen mitochondria with cristae blurred appeared in some pyramidal cells. Some pyramidal cells showed degenerative changes with some disappeared endoplastic reticula and ribose nuclear protein and increased lysosomes. The amount of extends endothelial projections going to the lumen of capillaries were increased.

同时伴有超微结构的改变，如神经细胞线粒体肿胀，内质网扩张，染色质凝集成块状，毛细血管外间隙扩大，髓鞘变性坏死，微管排列紊乱，小胶质细胞有吞噬变性坏死的神经纤维成分等。

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中，在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养，通过免疫荧光技术检测干细胞标志物CD133、Nestin，诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验，对其干细胞特性加以鉴定，得到如下结果：不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞，能表达干细胞的标志物CD133和Nestin，符合干细胞的超微结构特点，体外培养能连续传代；具有多向分化潜能：诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞；移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。