染色质核仁

Chromatin has been set shrunk to thin linear structure, each chromosome and the nuclear membrane through the attachment plate connected to increase the size of this nucleus, nucleolus rate as well.

染色质已集缩成细长的线状结构，每条染色体通过附着板与核膜相连，此期核的体积增大，核仁也较大。

Cytology reveals cells with clear to lightly basophilic cytoplasm and round or indented nuclei with fine chromatin and indistinct nucleoli.

细胞学分析可见透明至浅染的酸性细胞质和圆形或缩进的细胞核含有正常的染色质和模糊的核仁。

The neoplastic epithelia are low cuboidal and not columnar in shape, with very large and atypical oval nuclei with vesicular chromatin and prominent nucleoli.

肿瘤性上皮呈低立方状而不是柱状，有很大的卵圆形核和显著核异型性，空泡状染色质和显著核仁。

Figure5 HE 10×10 The tumor cells are large and have rich cytolymph, red coloration. The nucleolus is round or elliptical shape.

图5 HE10×10 肿瘤细胞较大，胞浆丰富，红染，核圆或椭圆形，染色质细，核仁及核膜清楚，背景可见较多成熟淋巴细胞、嗜酸粒细胞，散在巨噬细胞。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型，胞质丰富，胞核成圆形或椭圆型，核仁清楚，甲苯胺蓝呈异染性，阿尔新蓝8Gx 染色阳性，细胞外基质Ⅱ型胶原免疫组化染色阳性，Ⅰ型胶原染色阴性。

All cells were generally smaller and occasionally spindled, the chromatin detail was attenuated, and nucleoli were more prominent.

所有的细胞都稍小，偶见梭形，染色质不清，核仁突出。

A few of intercellular electron-dense zones without typical synapse were found in the inner plexiform layer.

内核层内可见胞核各异的多种细胞，部分胞核常／异染色质例接近，核仁偏心分布。

Transmission electron microscope results revealed vascular endothelial cells with normal morphology in the control group and tumor cells with apparent nucleoli in the Ad-GFP group, while in the Ad-aMMP-2 group, some vascular endothelial cells exhibited chromatin pycnosis in the nucleus, forming apoptotic bodies.

透射电镜观察对组血管内皮细胞形态正常；Ad-GFP组瘤细胞大，核仁明显；Ad-aMMP-2组部分血管内皮细胞核内染色质固缩，聚积成团块状形成凋亡小体。

Ultrastructure observation revealed that the nuclei became deformed, nucleolus disintegrated and chromatin condensed, the thylakoids of chloroplast swelled, the chloroplast membrane disrupted, cristae of mitochondria disappeared and mitochondria vesiculated.

电镜观察发现，Cd(上标 2+)处理下，细胞核变形，核仁解体，染色质凝集；类囊体片层膨胀，叶绿体双层膜破裂；线粒体中嵴突减少或消失，线粒体空泡化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。