染色质

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

RESULTS Gastric and duodenal epithelial cells and crypts showed noticeable positive staining in normal controls.

结果 iNOS染色定位于胞质，在正常人胃及十二指肠粘膜细胞和腺体均有表达。

RESULTS: Alkaline phosphatase activity: Alkaline phosphatase staining of cells was apparent; gray-black particles or massive precipitations were observed in cytoplasm after positive reaction; regions expressing alkaline phosphatase activity were brown-black.

结果：①碱性磷酸酶活性：经诱导后细胞碱性磷酸酶染色明显，胞质中刚性反应呈现灰黑色颗粒或块状沉淀，碱性磷酸酶活性部位呈棕黑色。

Chinalizarin staining was used to indicate the formation of mineralization tubercles in the cultured MSCs treated with WSM and condition medium.

施加处理因素第7d，WSM组及条件培养基组骨髓基质细胞AKP染色明显，与对照组相比差异显著。

RESULTS:① Both induced osteoblasts and MSCs exhibited ALP and calcium node stainings positive.

结果：①诱导成骨分化的骨髓基质干细胞分泌的碱性磷酸酶及钙结节染色均呈阳性。

Results Positive stain of bFGF mRNA, bFGF and FGFR1 were observed in both stroma and epithelium of the examined prostates.

结果 前列腺间质细胞和上皮细胞中均见b FGFmRNA、bFGF和FGFR1阳性染色。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF. 10 days later after the administration of PLGA microspheres, the effects of stimulus to synthesis of type Ⅱ collagen and proteoglycan can be compared with continue administration of free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短；bFGF缓释微球能通过持续释放活性bFGF，在较长时期内促进软骨细胞的分裂增殖；Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示，应用微球10天后，能有效的促进软骨基质成分的合成，其效果与持续应用游离bFGF的效果相当。

The bioactivity of bFGF released from PLGA microspheres are higher than from PELA.(2) the bFGF released from PLGA microspheres can stimulate mitosis, proliferation, and matrix synthesis of chondrocyte more effectively than free bFGF.

游离bFGF对软骨细胞的促有丝分裂的作用效应期短；bFGF缓释微球能通过持续释放活性bFGF，在较长时期内促进软骨细胞的分裂增殖；Ⅱ型胶原免疫荧光测定和蛋白多糖阿新兰染色测定显示，应用微球10天后，能有效的促进软骨基质成分的合成，其效果与持续应用游离bFGF的效果相当。

Results The articular cartilage constructed by the technique of centrifuge tube culture was hyaline cartilage.

结果在体外培养第2周时，组织切片可见软骨样组织结构；第3周时软骨发育趋于成熟，经阿新蓝染色基质丰富；第16周时，组织形态无明显变化，未见钙化。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。