染色质

The change of α1,2-FT activity in the cell line before and after the tranfection was confirmed by the determination of enzymatic activity. The changes of cell lipid and glucolipid, especially the change of type Ⅱ oligosaccharide, in the cell line before and after the transfection was determined by Thin-Layer Chromatography and TLC immunostaining method, respectively.

通过酶活性测定证明转染前后细胞系α1，2-FT活性的改变，采用薄层层析、薄层层析免疫染色方法测定转染前后细胞脂质及糖脂，特别是Ⅱ型寡糖的变化。

Observation of cultured cells stained by haematoxylin and eosin: It was shown that the cells presented round or ellipse. There was little cytoplasm, presenting acidophily. Nucleus was big and round, single in the center, deep stained.

培养细胞苏木精-伊红染色观察结果：可见细胞圆形或椭圆形，胞质少呈嗜酸性，细胞核大而圆，单个居中，着色深。

Methods:MTT assay was used to detect PGPG homotypic adhesion and PGmatrix adhesion,and PGHUVEC adhesion was determined by Rose Bengal stain.

小檗碱（5、10 μg/ml）处理PG细胞24小时，采用MTT法观察PG细胞与PG细胞的黏附，PG细胞与细胞外基质（FN和Martrigel）的黏附；用虎红染色法观察PG细胞与人脐静脉内皮细胞的黏附。

Histopathology of the hyperpigmented lesion revealed increase of melanin in the basal layer, pigment incontinence and amorphous eosinophilic masses stained positive as with Congo red in the papillary dermis.

色素沉著的病灶其组织病理显示在基底层有黑色素的增加，乳突状真皮出现色素失调和均质的嗜伊红性块状物质沉积，且以刚果红染色呈阳性反应。

Methods:The subcutaneous adipose tissue was obtained from the fold inguen of four SD rats, then digested with collagenase type Ⅰ and cultured in endothelial medium for seven days.

获取4只雄性SD大鼠鼠蹊部皮下脂肪，通过机械分割和组织消化法获取脂肪基质细胞，在内皮细胞培养液中培养7d后，经免疫细胞化学及透射电镜鉴定证实95%以上细胞已具内皮细胞表型特征，再将这些细胞进行成脂(DMEM/F-12培养基内加入0.5mmol/L1-甲基-3-异丁基-黄嘌呤，1μmol/L地塞米松，10μmol/L胰岛素，200μmol/L吲哚美星)定向诱导7d，形态学观察和油红O特殊染色鉴定诱导后的细胞。

Methods: After transferred pcDNA3.1/hTM plasmid into rabbit artery by high-pressure injection, rabbit common iliac artery were cut and anastomosed again. At the 14 days and 28days after second operation, we checked inside diameter of anastomotic stoma and blood flow velocity by color Doppler. The treated artery were sliced and stained by Verhoeff. Local neointima formation and the ratio stenosis of intervascular were calculated by computer.

用注射式加压转染的方式对兔动脉壁转染pcDNA3.1/hTM质粒，再制造动脉损伤-阻滞模型，于术后14天、28天用彩色多普勒观察活体吻合口内径和血流流速；再做病理切片Verhoeff染色，观察血管内膜增生的程度、部位，计算血管内膜面积、中膜面积和血管狭窄率。

Objective To investigate the distribution of intrarenal lymphatic vessels in developing rat using the lymphatic marker LYVE-l.

目的 探讨淋巴管内皮细胞透明质酸受体－1染色阳性LYVE-1(上标+淋巴管在大鼠肾内的分布。

Compared with control group,the ADM level in the left lung increased(P＜0.001),but the ADM levels in the heart,liver,kidney decreased.The ratio of wet to dry weight of the left lung in experiment group was 6.491±0.248,and 5.937±0.361 in control group,there was no significant difference（P＞0.05）.No alveolar wall thicken,alveolar and mesenchyma edema were found in two groups.

实验组兔左肺的湿/干重量比为6.491±0.248，右肺的湿/干重量比为5.937±0.361，双肺的湿/干重量比差异无显著性（P＞0.05）；HE染色结果显示实验组与对照组均未见肺泡壁增厚、间质及肺泡水肿等改变。

Methods Both in malformed embryos induced by excess retinoid acid and normal KM mice, expressions of lectin receptors of WGA, RCA and ECL in the epithelium of palatal shelves and the mesenchyma were determined by affinity histochemistry using biotin-marked corresponding lectins.

以生物素标记的凝集素受体WGA、RCA和ECL为探针，应用亲和细胞组织化学染色方法检测上述3种凝集素受体在RA致畸鼠胚和相应对照组鼠胚腭板上皮及其间充质的分布状况和变化规律。

Results: All the slides of the breast carcinoma in our department were reviewed, 9 cases of glycogenich, clear cell carcinoma, 4 cases of mucinous carcinoma, 2 cases of tubular carcinoma, 18 cases of metaplastic carcinoma, 6 cases of apocrine carcinoma, 1 case of secretary carcinoma, and 2cases of lipid-rich carcinoma were found.

结果：回顾42例乳腺癌病理切片，发现富含糖原的透明细胞癌9例，黏液癌4例，小管癌2例，化生性癌18例，大汗腺样癌6例，分泌型癌1例，富脂质癌2例；不同组织类型的乳腺癌具有不同的光镜、特殊染色及电镜表现。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。