染色质

Microinjection and particle gun were used to transfer pCMV-β into fertilized zebra fish eggs. After staining analysis, the data showed80% and 6% expression rates, respectively.

此研究利用显微注射与粒子枪基因转殖法转殖pCMV-β质体进入斑马鱼授精卵，经染色分析得到80%与6%之表现率。

Oral administration of DNA nanoparticles synthesized by complexing pCMVβ DNA with chitosan,which modified with gelatin,Sodium Alginate,PEG and acetylize respectively,resulted in transduced gene expression in the intestinal epithelium.

分别使用明胶、海藻酸钠、PEG及乙酰化修饰包裹含LacZ的质粒pCMVβ的壳聚糖纳米颗粒，通过口服发送后经X-gal染色检测目的基因在小鼠体内的表达。

This is the microscopic pattern of a small cell anaplastic carcinoma in which small dark blue cells with minimal cytoplasm are packed together in sheets.

这是肺小细胞癌的镜下形态学改变，图示可见小的染色较深、胞质较少的癌细胞成排排列。

Results: Neonatal rats in hypoxic-ischemicgroup behaved restlessness, cyanosis, deep and rapid breath, astasia, lethargy, irritation and spasm; There were local pyknosis, fragmentation, dissymmetry, blur or disappearance of nucleoli, raritas of ground substance in left cerebral cortex; but there was no statistic significance in chinese traditional medicine group and sham group. The expression of HIF-1α mRNA in brain tissue of experimental group and chinese traditional medicine group were higher than that in sham group, especially chinese traditional medicine group at 3d; HIF-1α positive cells were found in cerebral cortex and hippocampus. There were more HIF-1α positive cells in experimental group and chinese traditional medicine group and mainly in vascular endothelial cells.

结果 缺氧缺血后大鼠相继表现为烦躁不安、全身发给、呼吸加深加快、站立不稳、嗜睡、激惹或间断发作的痉挛和抽搐；HE染色显示实验组大鼠左侧大脑皮层出现局灶性神经元核固缩，核碎裂，核仁偏位，不清或消失，基质疏松；中药组大鼠脑组织结构基本接近假手术组，无明显水肿和坏死表现；实验组和中药组大鼠脑组织HIF-1α mRNA表达较假手术组明显增加，尤其是中药组实验第3日者；免疫组化显示实验组和中药组各时间点大鼠大脑皮层和海马区均可见不同程度的HIF-1α表达，明显高于假手术组，阳性表达主要在血管内皮细胞，海马和皮层的锥体细胞亦有HIF-1α阳性细胞分布。

Autoradiography of aortas demonstrated uptake of the agent into macrophage-rich atheromata identified by Oil Red O staining of lipid deposits.

以油红O染色确定脂质沉积处的动脉粥样硬化瘤，放射自显影技术显示该瘤中富含巨噬细胞并摄取显像剂，荧光镜和流式细胞仪显示主动脉的细胞中主要是巨噬细胞摄取显像剂。

Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology.

背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组，给药组涂抹红鹿制剂，对照组涂抹化妆品基质，连续涂抹60 d；应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。

Results The expression of CgA was detected in cortex of adrenocortical adenoma, adrenal neoplasm and chromophile tumor, but not in that of hyperplasia and normal adrenal.

运用免疫组化方法对肾上腺肿瘤病例进行CgA、NSE和SYN染色，观察其在皮髓质中分布特点。

A trichrome stain highlights the collagenous connective tissue of pulmonary fibrosis in blue.

这是肺间质纤维化的三色染色的镜下图片，可见肺组织的纤维化区被染成蓝色。

The model mice were then treated by giving GDNF into the striatum. After a seven-day survival period, the model mice were sacrificed to get the segment of substantial nigra fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry using anti -TH and anti-CB antibodies to label DA neurons and CB-containing neurons respectively, which were counted under microscope and analyzed statistically.

利用1-甲基－4-苯基1，2，3，6-四氢吡啶(1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine，MPTP)制备成年小鼠PD模型，通过单侧纹状体定位注射GDNF，取中脑黑质节段，做连续冠状石蜡切片，结合免疫组织化学染色方法，观察各组酪氨酸羟化酶(tyrosine hydroxylase，TH)、钙结合蛋白(calbindin D28k，CB)的表达，光镜观察并细胞计数，统计学分析。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。