染色质

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

Callose staining with particular fluorescence dye-Aniline blue indic-ated that the degradation of callose was delayed.

利用胼胝质特异性荧光染料甲基蓝对花药进行染色，表明突变体胼胝质降解延迟，导致小孢子不能从四分体中释放出来，最终没有花粉粒的形成。

The patient has a history of chondrosarcoma and now, this is recurrent/metastatic chondrosarcoma.

本例用这种染色特别容易认识肿瘤产生的基质，本例肿瘤就是富于粘液样软骨基质。

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示，未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色，核区较暗；CDDP及β-Ele处理组细胞为暗绿色，其中可见皱缩的凋亡细胞；THP处理的细胞胞质荧光弱呈灰绿色，由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和，胞核被染成桔红色，结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

488Anti-rat CD106 and Bis-Benzimide staining to calculate cytoplast purity. Mitochondrial membrane potential was detected by flow cytometry following labeling with Rhodamine123. RESULTS: The purity of cytoplasts was up to 95%.

488anti-rat CD106及Bis- Benzimide染色进行形态学观察，计算胞质体纯度；应用Rhodamine123标记后用流式细胞仪检测胞质体中线粒体膜势能。

The bulk of cells in the positive control group were dead.2 Treated with different dosages of DMF for 24h, it can be observed that green,nuclear margination and chromatin condensation were distinct in cells exposed to DMF.

研究结果 1。二甲基甲酰胺致人肝细胞凋亡形态学观察： 1不同剂量DMF染毒12h后，AO／EB染色可见阴性对照组无明显凋亡细胞，50mmol／L剂量组开始出现细胞形态接近正常，胞质为亮绿色，核出现固缩呈圆珠状，与正常细胞比较核质比变大的细胞。

To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm.

经RT-PCR验证得知SPANXA1在CL1-0的表现量比CL1-5多100倍以上，於是我们将SPANXA1转染在CL1-5细胞株中，并测量活体外的细胞生长速度试验、细胞迁徙实验、Matrigel侵袭实验、癌细胞群落形成实验及小鼠活体内细胞转移能力，探讨SPANXA1对癌细胞性状的影响，结果发现增加SPANXA1会降低癌细胞的转移能力，在另一方面，我们利用免疫萤光染色法侦测出SPANXA1是存在於核质中，并以西方点墨法再次证实SPANXA1是存在於核质中。

RESULTS: Levels of insulin, FFA, GOT and GPT in serum were significantly increased in model group; activities of T-SOD and GSH-Px in liver tissue and ISI were notably decreased, and content of lipid perhydride MDA was increased. HE staining revealed that there was hepatic cellular swelling in hepatic lobules, inequality of size of lipid droplets in periplast, nucleus on one side, narrowed sinus hepaticus and inflammatory cell infiltration in hepatic lobules and portal area.

结果： 模型组大鼠血清胰岛素、FFA、GOT和GPT水平明显升高，肝组织中T-SOD和GSH-Px活力和ISI明显下降，脂质过氧化物MDA含量显著增加， HE染色示肝小叶中大部分肝细胞肿胀，胞质中出现大小不等的脂滴空泡，以大泡性脂肪滴为主，核居边，细胞界限不清，肝窦狭窄，且存在小叶内及门管区炎症细胞浸润。

RESULTS: The positive staining cells mostly included ependymal cells of myelocoele, glial cells, motor neurons, and epithelial cells of spinal pia mater. The staining was mainly located on the cell membrane. Most importantly, it displayed certain directivity in the glial cells, mainly at the membrane side contacting with capillary endothelial cells.

结果： 免疫组织化学染色结果显示阳性着色细胞包括有胶质细胞、中央管室管膜上皮细胞、脊髓前角运动神经元、软脊膜上皮细胞，着色部位主要在细胞膜，其中在胶质细胞表现出一定的方向性，主要分布在与毛细血管内皮细胞接触的一侧。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。