染色质

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

All rats were sacrificed at 12 weeks after operation. the macro-pathological changes of samples were graded by mankin's scale.bone mineral density of right distal femoras and femora condyles were measured by dual-energy x-ray absorptiometry scanner.

术后12周处死动物取材，采用mankin评分系统评分；用双能骨密度仪测量右侧股骨远端1/4骨密度和股骨内外侧髁软骨下骨的骨密度；右侧股骨髁脱钙后切片，行番红&o&染色和mmp-13免疫组化染色；右侧胫骨近端做硬组织切片，测量软骨下松质骨的骨组织形态计量学参数。

Serum estradiol level of rats was detected by means of radioimmunoassay. The positive staining and intensity of lipid in the meibomian gland alveolus was detected by Sudan Ⅲ staining.

实验评估：采用放射免疫分析方法检测血清雌二醇水平；采用组织化学苏丹Ⅲ染色方法对睑板腺腺泡上皮做脂质染色阳性和强度检测。

Methods The neuroepithelial stem cells were isolated and cultured by microdissection, mechanical blowing and serum-free suspension culture.

方法采用显微解剖、机械吹打、无血清悬浮培养方法分离培养神经上皮干细胞，采用巢蛋白免疫细胞化学染色技术检测神经上皮干细胞，用NSE和GFAP免疫组化染色检测并计数神经细胞和神经胶质细胞。

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

And Sirius Red in a supersaturated picric acid solution viewed by polarization microscopy. The changes of mRNA expression of collagen type I were analyzed by RT-PCR. The results were compared with those after intervention with Losartan.

染色及苦味酸天狼猩红胶原特异染色偏振光显像进行心脏间质胶原的组织化学观察，RT-PCR观察心脏I型胶原mRNA表达的变化，并与氯沙坦干预后比较。

Methods We reported a case of PXA located in the cerebellum of a young adult in view of its extremely unusual location. Morphologic observation, immunohistochemical staining using DAKO EnVision system , histochemical stains including hematoxylin eosin and Gordon Sweet's reticulin methods were used.

采用免疫组织化学DAKOEnVison法、组织化学网质纤维染色和HE染色及形态学观察的方法，报道 1例极罕见于年轻人小脑的多形性黄色星形细胞瘤，探讨其临床病理特点。

In normal group, the staining of FN formed intact linear structure at basement membrane and presented regular striae form in connective tissue.

正常组织基底膜中FN染色呈完整线性结构，结缔组织间质中FN染色呈规则的条纹状。

Results as follows;(1) At 25th week of fetal age, the staining intensity and cell density almost equalled to that at birth in cervical spinal cord, brainstem, hippocampus, and vermis of cerebellum; while there are only 1/4 cell densities in cerebral cortex.

用胶质原纤维酸性蛋白抗体进行免疫组织化学染色。结果表明：(1)在颈段脊髓、脑干、海马和小脑蚓部于胚胎25周其GFAP染色强度、细胞密度接近出生时水平。

It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion-induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions.

采用硅管套接大鼠切断的坐骨神经模型，局部给予胶质细胞源性神经营养因子，应用尼氏染色、酶组织化学染色方法，观察到外源性GDNF能减少脊髓修复侧前角运动神经元死亡的数目，降低脊髓前角运动神经元及脊神经节感觉神经元中胆碱酯酶及酸性磷酸酶变化的幅度。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。