染色质

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

The function of plant genomes depends on chromatin marks such as the methylation of DNA and the post-translational modification of histones.

植物基因组的功能依赖于染色质标记，诸如DNA的甲基化和组蛋白的转录后修饰。

Covalent modification of histones on chromatin is a dynamic mechanism by which various nuclear processes are regulated.

染色质上组蛋白的共价修饰是一个动态的机制，多种细胞核过程通过该机制被调控。

Studies on many aspects show that there are four main reasons which cause the epigenetic effects;the methylation of DNA,the acetylation of histones,the change of chromatin structure and the alteration of protein configuration.

多方面的研究表明，导致后生效应的可能原因主要有四个方面：DNA的甲基化、组蛋白的乙酰化、染色质结构背景的改变和蛋白质构型的变化。

When coupled with overexpression of Delta, deregulation of the expression of Pipsqueak and Lola induces the formation of metastatic tumours.

这个过程需要有两种组蛋白修饰酶和染色质域蛋白Polycomb的存在。

Furthermore, the abnormality of PARP's activity and chromatin structure in leukemia cells was detected. Possible significance of this result was discussed.

在此认识的基础上，初步研究了PARP在白血病细胞中的异常活性，白血病细胞染色质结构异常及其可能的意义。

Observations underelectron microscopy showed that YSV destroyed the structure of chromatin,damaged cell organelles of tumor cells, and induced apoptosis and necrosis intumor cells.

超微结构研究显示，YSV(320μg／kg／d)诱导人肝癌BEL-7402细胞的染色质溶解、固缩，细胞器的普遍崩解，细胞出现凋亡和坏死。

The other one was cultured for retinal progenitors. Then neurosphere-like cell clusters were observed by TEM.

结果：在胚胎5月的人视网膜神经上皮层细胞的细胞核内出现了大量的异染色质。

The germinal vesicle can beisolated successfully in such a physical method and it can undergo chromatincondensation in vitro just as the oocyte does in vivo.

用直接取核法可成功分离出游离的生发泡，且它们在体外培养条件下可以和体内一样发生染色质凝集现象。

DNA fluorescence dye Hoechest 33342 was used tomonitor the condensation of chromatin during germinal vesicle breakdown.

采用直接取核法获取卵母细胞生发泡，用同步化的HeLa细胞裂解液构建非细胞体系，将游离的生发泡培养在裂解液中，并加入荧光染料Hoechest 33342，观察染色质的变化情况。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。