染色质

Previous studies show that p33ING1b is involved in growth inhibition, apoptosis, chromatin remodeling, DNA repair, tumor suppression and cellular senescence, but its biochemical function and involvement in cellular senescence is still not fully uncharacterized.

p33ING1b是一个较晚发现的肿瘤抑制基因 ING1的主要表达形式，自从被成功克隆以后得到了广泛的研究，已有的研究表明，p33ING1b参与了细胞的生长抑制、凋亡、染色质重塑、DNA损伤修复、肿瘤抑制和细胞衰老等。

RDNA in different positions existed in either condensed or uncondensed forms. Nucleolus-associal-ed chromatin outside nucleolus extended in the periphery of FC via channels in the nucleolus.

不同位置的rDNA成分都具有集缩和解集缩两种形态结构，核仁外的核仁伴随染色质经过核仁通道进入核仁，沿FC周边排列，与其中的DNA相连。

In eukaryotic cells, the fundamental unit of chromatin is nucleosome, which is composed of a histone octamer with two copies of H2A, H2B, H3 and H4 and 146 base pairs of DNA.

真核细胞中，核小体是染色质的基本单位，它由两个拷贝H2A、H2B、H3和H4所组成的核心组蛋白八聚体，及缠绕于其上的146bp DNA构成。

The over expression of p33^ING1b can promote the expression of p21^WAP1 which cooperates with p53 to restrain the cell growth by regulating the activity of the acetylating depended pathway.

ING1编码的蛋白通过组蛋白乙酰转移酶和组蛋白去乙酰化酶参与染色质的重塑，在DNA损伤修复方面发挥重要作用。

"Acetylating H3K4 and demethylating H3K9 can actiate a gene; deacetylating H3K4 and methylating H3K9 can silence it," Karpen explains."Multiply modified at arious locations, these histones are a major factor in changing the functions of chromatin, independent of DNA sequence."

&H3K4的乙酰化和H3K9的去甲基化使基因活化；而H3K4去乙酰化和H3K9的甲基化则使基因沉默，& Karpen讲解道，&组蛋白的多部位多重调节是改变染色质功能的主要因素，并与DNA的序列无关&

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

In summary, the BRD7 gene acted as a candidate of tumor suppressor gene with NPC. The overexpression of BRD7 can partly reverse malignant phenotype of NPC cell line. The suppression effect of BRD7 on NPC tumorigenesis my be achieved by recognizing acetylated histone peptide through their motif-bromodomain, then modulating gene transcription by taking part in histone acetylating and chromosome remodeling, finally influencing signal-transduction pathways.

综上所述，BRD7基因作为一个重要的鼻咽癌抑瘤基因侯选者，在鼻咽癌细胞中的过表达后，可导致鼻咽癌细胞 HNE蛋白质表达谱发生改变，逆转其恶性表型，其作用机理可能是：BRD7基因通过其功能域彭聪硕士学位论文10Bromodomain与乙酚化的组蛋白特异性结合，参于染色体的乙酚化，染色质的组装，从而影响基因转录的调控，最终影响细胞内的信号传导通路并实现对细胞周期的调控，从而发挥抑制鼻咽癌细胞生长的作用。

Apoptosis of myocytes was observed in the peripheral infarcted area, which appeared as shrinkage of cells and a condensation or marginating of chromatin. Large fragmentations and ladder bands were seen in DNA gel electrophoresis.

梗塞边缘区可见凋亡心肌细胞，其形态表现为细胞皱缩，细胞核染色质聚集成块或边集；DNA凝胶电泳可见大片段或梯形带。

Chemical modifications to DNA and histone proteins form a complex regulatory network that modulates chromatin structure and genome function.

DNA和组蛋白的化学修饰形成了一个调节染色质结构和基因组功能的复杂调控网络。

The cleaved form of SSH3 translocated from cytosol to nuclear and bound to chromatin.

在细胞凋亡过程中，这种剪切形式的SSH3可以从细胞质转移到细胞核，并结合到染色质。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。