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染色质

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It is realized that chromatin structure of a gene has an on and off effect for the expression of gene.

染色质结构在基因调控中的重要作用越来越为人们所认识。热休克蛋白以其对热诱导的迅速反应成为研究染色质水平基因调控的重要模型。

New methods for the genome-wide analysis of chromatin are providing insight into its roles in development and their underlying mechanisms.

染色质的基因组范围分析的新方法正在提供对染色质在发育和其根本机制中作用的深刻见解。

Chromosome segregation and cytokinesis must be tightly coordinated to ensure that chromosomes are accurately partitioned between dividing cells.

染色质隔离和胞质分裂必需紧密地协调,从而确保染色质在分开的细胞间准确的分离。

The neuron apoptotic cells were observed by their fragmented nuclei condensed chromatin, fragmented or condensed cytoplasm and floccule of mitochondrion in the control group after ischemia 90 min/ reperfusion 12 h; whereas the distribution of chromatin was well-proportioned, and normal mitochondrion and other organelle were observed in the Ginkgo Biloba Extract-treated group.

对照组缺血90 min再灌注12 h鼠脑组织电镜检查可见神经元细胞核染色质边聚,呈团块状,线粒体可见絮状结构改变;相同时限药物治疗组脑组织神经元细胞核染色质分布比较均匀,胞质内线粒体及其它细胞器基本正常。

Several masses of condensed chromatin without nuclear membrane were observed in some treated K562 cells,while chromatin of some other cells tended to marginate in crescents around the nuclear envelope with compacting cytoplasm,normal or slightly expanded endoplasmic reticulum,which presented typical morphology of apoptosis.

电镜下,加药处理组中部分细胞染色质凝集成块,没有核膜包被,部分细胞染色质靠核膜边集呈新月形,胞浆浓缩,内质网结构正常或有扩张,呈现典型的凋亡细胞形态。

Thirdly,The nuclear chromatin of lung carcinoma cells concentrate to periphery, the electron density of cytoplasm increased, in some cells,the heterochromatin of nucleus were blurry,and the nucleole became uniform in electron density, the formatio reticularis of nucleole disappeared.

细胞核的改变:细胞核内染色质边集,基质电子密度增高。有些细胞核内异染色质分界不清,核仁呈均一电子密度,网状结构消失。

Results Lactacystin dealed with C6 glioma cells in 24 h, kytoplasm was concentrated and anachomasised, the chromatin of nucleus was concentrated pyknoticly to see under photo-microscope. The microvilli on surface dispeared、nucleus crenated、chromatin aggregated pyknoticly under transmission electron microscope respectively.

结果 Lactacystin处理体外C6胶质瘤细胞24 h后,光镜下见胞浆浓缩深染,胞核染色质致密浓缩,透射电镜下见细胞表面微绒毛消失,核皱缩,染色质致密浓缩、边集。

The gland secreted in a holocrine fashion,It had two types of cells:type A and type B.

腺体进行全浆分泌,有A、B两种细胞类型,A型细胞具常染色质,粗面内质网和线粒体丰富,B型细胞具异染色质,细胞核固缩。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果:细胞接触肽段后存活率下降28.9%;光镜下可见细胞贴壁不良,胞体缩小,细胞突起断裂缩短;荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞;电镜下可见胞质中出现空泡样结构,细胞染色质浓集于核膜内侧并裂解成碎块状;流式细胞仪检测细胞出现亚二倍体峰,DNA电泳出现梯状带。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现,次生壁形成早期,细胞核具有双层核膜,染色质凝聚,可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中;随后,双层核膜消失,细胞器将逐渐解体,多泡体开始出现在纤维细胞的细胞质;随着年龄的增加,纤维细胞壁逐渐增厚,并出现多层结构现象,而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

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