染色质

Results Of 52 AL patients, 28 were patients with diploid, 22 were patients with heteroploid and 2 were patients with suspected heteroploid. The prevalence of heteroploid in AL patients was 42.3%.

恶性肿瘤的重要特征之一就是细胞无限制地增生，在形态学上表现为细胞核增大，核内染色质增多，这是核内DNA不断复制的结果。

HIRA may function in regulating chromatin structure, nucleosome reassembly and cell cycle progression by the interactions of its different domains with different proteins.

HIRA可通过其不同的结构域与不同的蛋白质相互作用，来参与调节核小体的组装、染色质的结构及细胞周期的进程等。

Our data suggest that these histone modifications may act cooperatively to prepare chromatin for transcriptional activation.

我们的数据表明，这些组蛋白修饰可能共同作用于染色质的转录激活。

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

NGF had obvious cytotoxicity on these cell lines ,which shows to be dose-dependent, especially for MGC-803 and BEL-7404 ,their IC_(50) value at the 24~ hours are 49.9mg/L and 55.46mg/L ; Main features of apoptosis under fluorescent microscope include reduction in volume,nuclear chromatin condensation, intensification under jacinth fluorescence; The early apoptosis rate of pristine cells which detected by FCM increases with NGF dose increasing.

2NGF对4种人肿瘤细胞均有细胞毒性作用，且呈剂量依赖关系，尤其是对MGC-803和BEL-7404的抑制作用更强，作用24h的IC50值分别为49.9mg/L和55.46mg/L，荧光显微镜下可观察到MGC-803凋亡细胞的主要特点为：细胞体积缩小，染色质固缩，橘红色荧光增强；流式细胞仪检测到MGC-803细胞的早期凋亡率随NGF浓度的增高而增加，各浓度组与对照组的早期细胞凋亡率比较有明显差异(P＜0.01)。

It showed that thymidine could have special effect on cells in that replication in cells suffering thymidine treatment slow down substantially but do not stop DNA synthesis. This phenominon of leakiness in replication blockage provided chances for oligonucleotide to mediate repair.

通过检测上述三种抑制剂作用于细胞后不同时间的细胞周期分布，发现thymidine在抑制DNA合成的同时，存在着缓慢的渗漏作用，即染色质DNA上复制叉缓慢移动。

RESULTS: There were many vacuoles and cracks in the cells, with heterochromatin margination, nuclear pycnosis and nuclear fragmentation. The high intensity pulsed electric fields caused damage in K562 cells, and the apoptotic percentage went up with the increase in frequency and intensity of PEF.

结果：经过高电压脉冲电场处理后的K562细胞，胞内出现大量空泡及裂隙，核内染色质向核周边聚集形成凋亡小体，呈典型细胞凋亡形态学改变；K562癌细胞的凋亡比例随着脉冲个数和电场强度的增加而增加。

Observ ed with transmission electron microscope and scanning electron microscope, t he apoptotic nuclei showed the typical morphological feathers of apoptosis: cond ensation and margination of chromatin, bubbling of nuclear membrane and apoptoti c bodies.

在透射电子显微镜和扫描电子显微镜下都观察到凋亡的细胞核呈现典型的凋亡形态学特征，例如染色质的凝集及趋边化，细胞核表面形成空泡化结构并有大量的凋亡小体结构出现。

Results1.In diabetic rats,the pathological change of the retinal structure displayed the decrease of the retinal ganglion cells\' number,and the signs of the apoptosis in different degrees could be found in the altrastructure of retinal neurons,including:cell shrinkage,chromatin margination and nuclear collapse,and so on.

结果1。随糖尿病大鼠病程延长，逐渐出现视网膜神经节细胞数量减少、细胞排列紊乱的病理改变，透射电镜下可见视网膜神经细胞出现胞浆浓缩、细胞体积缩小、染色质边聚、细胞核固缩、断裂等不同阶段的凋亡征象。

The apoptotic effects of LPS are detected by various skills such as fluorescence staining, TUNEL reaction, DNA agarose gel electrophoresis, electron microscopy and fluorescence activated cell sorting. Our results demonstrated that fish lymphocytes would be apoptotic under different LPS concentrations(above 10μg/ml) and different time(above 6h) inducing, which is characterized by condensation and margination of chromatin, the formation of apoptotic bodies.

结果显示，在10μg/ml以上浓度的内毒素作用18后或以100μg/ml浓度的LPS作用6h以上，淋巴细胞即可出现明显的凋亡形态学变化，表现为染色质凝集和周边化，核断裂，形成凋亡小体，DNA发生特异降解产生典型的"梯状"电泳图谱，呈现TUNEL阳性标记等，并通过流式细胞术证实这种凋亡具有时问和剂量的依赖性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。