染色粒

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株，经筛选后倒置荧光显微镜观察细胞形态及荧光状态，挑取荧光最强的克隆，命名为ES-GFP细胞，进一步扩增培养，观察细胞生长及荧光表达情况，行细胞分化全能性鉴定包括碱性磷酸酶（alkine Phosphtase，AKP）染色、体外胚胎体（embryonic body，EB）形成实验及裸鼠体内成瘤实验。2。

The result shows that the vesicle diameter first increase and then decrease with the increase of AOT mole fraction, the change regularity of which is the same with the depth of apparent blue color. The vesicle size increases with the increase of total surfactant concentration at any mixing ratio.

新制备的PTA／SDS=3／7，总浓度为0.01M的样品，负染色透射电镜观测发现了管状和球状结构的囊泡，球状囊泡的粒径在50～200nm之间，管状囊泡结构直径在70～200nm之间，而长度大约为7000nm，但是类似的"管子"在新制备溶液中还是比较少。

Alkaline lipase Greasex 50L is one of the leather enzyme products of Novo Nordisk AS. After extensive control trials on pigskins, evaluating effects by degree of fat removal and qualities of result leather, we got1 Fat removal rate can be increased by 10%and leather qualities such as napping and softness can be improved by using Greasex 50L together with liming enzyme NUE 0.6 MPX2 Some surfactant together with Greasex 50L is good to fat removal, and the recommended ratio is 0.05%～0.1%∶13 The fat content in the skins is lower after treated with Greasex 50L,which also benefits the successive processes, such as bating, pickling and tanning. As a result, the leather qualities, such as fineness of grain, softness, napping and evenness of dyeing can be improved.

Greasex 50L是诺和诺德公司生产的碱性脂肪酶已在皮革工业中广泛使用，为了更好地应用这种酶制剂，我们对Greasex 50L在猪皮方面的应用条件进行了研究采用对比的方法，用脂肪去除率和皮革的质量作为评价标准，研究结果表明1适量的浸灰酶 NUE 0.6 MPX可使Greasex 50L的脱脂率增大，同时可改善皮革的起绒状况和革身柔软性2一定量的表面活性剂与Greasex 50L配合使用，有利于脂肪的去除，用量为平平加的0.05%～0.1%3 经Greasex 50L脱脂的裸皮在鞣制后油脂含量也较低，有利于后续工序进行，从而使成革粒面平细、革身柔软，臀部起绒得到改善，染色均匀性良好

Callose staining with particular fluorescence dye-Aniline blue indic-ated that the degradation of callose was delayed.

利用胼胝质特异性荧光染料甲基蓝对花药进行染色，表明突变体胼胝质降解延迟，导致小孢子不能从四分体中释放出来，最终没有花粉粒的形成。

Following the replication of chromosomes, resultant chromatids remain attached at the centromere.

随着染色体的复制，着丝粒始终连接着形成的两个染色单体。

Methods: The residual lymphoblast percentage of bone marrow was evaluated on day 14 during a four-drug induction in 21 children with ALL. Telomerase activity of bone marrow cell was determined by telomere repeat amplification protocol -silver stain technique at differential clinical stages.

以多药联合化疗方案进行诱导缓解和缓解后治疗，以诱导治疗第14天的骨髓原始淋巴细胞比例作为治疗反应敏感性指标，以端粒重复序列扩增-银染色技术检测端粒酶活性作为微小残留病白血病指标。

Objective To compare the two diagnostic methods（bone marrow fragments smear and bone biop-sy）in hematology diseases.Methods Take bone marrow using routine aspiration and make bone marrow fragments smears;using trephine take a piece of bone marrow send it to the pathology department have a histodiagnosis.

目的 对造血系统疾病进行骨髓小粒拉片与切片两骨髓检查的方法进行比较方法常规髂后上嵴骨髓穿刺抽取骨髓液制成涂片；取骨髓小粒制成拉片；再用环钻法取骨髓组织制成切片并作相应的染色，结合临床进行分析。

Soap soap is a carboxylate compound with excellent wetting, dispersing and washing power for a textile dyeing soaping and washing equipment fast.

皂粒皂粒是羧酸盐复合化合物，具有优良的润湿、分散和净洗能力，适用于纺织品染色后的皂洗和生产设备的快速洗涤。

All metaphases showed three distinct green signals, which have the same size and intensity, corresponding to a normal chromosome 8 and two isochromosomes 8q, and five red signals, of which one was located on a normal chromosome 8 and the another two quite symmetrically on the both arms of each isochromosome 8q, respectively.

结果发现：所有中期分裂相均显示3个绿色荧光杂交信号和5个红色荧光杂交信号；2个绿色荧光杂交信号分别位于2条等臂8q染色体着丝粒处，信号大小和强度均与位于正常8号染色体着丝粒处的1个绿色荧光杂交信号相当；1个红色荧光杂交信号位于正常8号染色体长臂上，另外2个红色荧光杂交信号非常对称地位于每一条等臂8q染色体的两条长臂上。

In most of these, two well-separated fluorescence foci were detected, indicating that the nucleoids had been actively pulled apart.

若这类贮藏物大量存在时，用碘使对其染色，肝糖粒能被碘液染成红色，淀粉粒被碘成蓝色。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！