染色粒

In addition, spermiogenesis includes chromatin condensation, migration of the cytoplasm and mitochondria to the caudal pole of the nucleus, formation of a moderate axial nuclear fossa containing the proximal centriole and part of the distal centriole and presence of a short cytoplasmic canal separating mitochondria from the flagellum.

另外，精子发生过程中还包括染色质浓缩，细胞质和线粒体向细胞核的尾端迁移，在核的后端中轴位置上形成中等大小的核后凹，近端中心粒和远端中心粒的一部分嵌在核后凹之内，短的胞质内陷管将线粒体与鞭毛分隔开。

In human spermatozoa, the positive immunofluorescent staining of centrin protein was seen to be located in the basal body of flagellum and clearly in proximal and distal centrioles.

健康男性精子中Centrin免疫荧光阳性，位于鞭毛的基体部，显示近端和远端中心粒，而Cenexin只在一个中心粒上有明显阳性染色，表明该蛋白是人成熟精子中心体的一种重要结构成分。这一结果显示了人和啮齿目动物精子中心体的差异。

The conversion boundary of self-incompatibility was researched by sowing by stage. Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃.HE97 was primarily regard as self-incompatible line in this research. The method was by inbreeding and sib HE97, Half the silk of HE97 and Zheng58. Inbreeding and cross have been done simultaneously. The extending condition of pollen tube was observed by dying the silk after pollinating 4 hours. The individuals with pollen tube were observed having seeds, while the individuals without pollen tube were observed having few seeds or no seeds. So this study regard HE97 as sporophytic self-incompatibility .The planting ration of female parent HE97 and male parent 87-1 was 4:1.The nature cross-pollination was studied by the theory of endosperm xenity, the study show that the setting percentage of HE97 was 625%, in which the seed in self was 70% and the seed in hybrid was 30%. This was consistent with the study of silk anatomy structure, then come to the same conclusion: compatible pollen germination and fertilization can stimulate the self-incompatible pollen germination and fertilization in the same corn cob.

通过分期播种，进行了光温敏自交不亲和材料HE97亲和性转换区间的研究，依据田间跟踪调查叶龄及室内考种研究了HE97亲和性转换区间的光温临界值，结果表明：1日最低温度是影响HE97自交不亲和性的主要原因，亲和性转换区间在16.5℃～18.1℃之间。2通过HE97自交和姊妹交以及将HE97与郑58作为双亲，各自的花丝一分为二，分别正反交，授粉后4个小时分别取其花丝进行固定、染色，观察花粉管的伸长情况，根据结实率与花粉管伸长的对应关系，结果发现观察到花粉管的对应植株收获到了种子，而没有观察到花粉管的对应植株结实率极低或没有结实，本研究把HE97初步划为孢子体自交不亲和类型。3以HE97为母本，87-1为父本，按4母：1父行比，通过花粉直感效应研究天然异交率，结果母本HE97结实良好(62%)，其中自交粒占70%，异交粒占30%，结合花丝切片观察表明，亲和花粉的萌发及受精会导致落在同一果穗柱头上不亲和花粉的萌发不再受抑制。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

It represents 99.4% of the euchromatic DNA and includes one megabase of heterochromatic sequence within the pericentromeric region of the short and long arm of the chromosome.

其中99.4％是常染色质，在着丝粒周围短臂和长臂区域还有1Mb的异染色质序列。

The signals look like noncontinuous beads on the DNA fiber,which consist of multi-copy and arrange tandemly.Fiber-FISH results with 45S rDNA probe showed that an average signal length is about 3～11μm in many tandem copy sequence(measure number,n=8),so we estimated the size of each copy to be approximately 11～30kb in cotton genome.There are dual signals on each end of the mitosis metaphase chromosomes and pachytene chromosomes hybridized with telomere DNA probe.The signals also look like non-continuous beads on the DNA fiber,the length is about 1～9μm,so we estimated the size is about 4～27kb.The signals almost covered the whole mitosis metaphase chromosomes and pachytene chromosomes of G.arboretum Shixiya 1 hybridized with gDNA probe,the euchromatin zone and heterochromatin zone were identified clearly on pachytene chromosomes,and the signals also look like non-continuous beads.Two BAC clones 150-D-24 and 182-F-9 in DNA BAC library of Pima90 were selected as probe to hybridize with mitosis metaphase chromosomes,pachytene chromosomes and DNA fiber of G.raimondii.

棉花FISH技术系统的应用研究。45S rDNA在亚洲棉石系亚1号中期和粗线期染色体上有两对大的信号，在DNA纤维上信号为非连续的念珠状，多个拷贝串联排列，每个拷贝长度大约在3～11μm，推测每个拷贝的实际长度为11～30kb；端粒序列在亚洲棉石系亚1号中期和粗线期染色体端部都有双点信号，在DNA纤维上信号也为非连续的念珠状，不同染色体上信号长度大约在1～9μm不等，推测实际长度为4～27kb；gDNA信号几乎布满整个亚洲棉石系亚1号中期和粗线期染色体，在粗线期染色体上能明显区分出常染色质区和异染色质区，DNA纤维上的信号均为非连续的念珠状。

There appeared prominent signals on pericentric regions of all the other chromosomes besides chromosome 4. The signals were strong on most of the chromosomes and weak only on one chromosome pair.

这一克隆除在第4染色体近着丝粒区显示明显的信号外，其它染色体的近着丝粒区同样具有信号，多数染色体为强信号，仅1对染色体信号较弱，还有3对染色体具有像着丝粒两侧异染色质块(pericentric heterochromatin block)的信号，中间有一个缢痕。

By means of experiments of marker transfer,acridine orange sensitivity test,F' curring and mini-chromosome transformation it is.conchi-ded that the F' plasmid is always in an integrated state in the Sin strains and that the initiation of the replication of the bacterial chromosome is carrie...

标记转移、吖啶橙敏感性，F′质粒消除和mini-染色体质粒转化等实验说明，Sin菌株中F′质粒始终处于整合状态，并且在40℃中细菌染色体的复制由整合状态的F′质粒所带动。

By means of experim ents of marker transfer,acridine orange sensitivity test,F' curring and mini-chr omosome transformation it is concluded that the F' plasmid is always in an integ rated state in the SIn strains and that the initiation of the replication of the bacterial chromosome is carried on by the integrated F' plasmid.

标记转移、吖啶橙敏感性、F′质粒消除和mini-染色体质粒转化等实验说明，Sin菌株中F′质粒始终处于整合状态，并且在40℃中细菌染色体的复制由整合状态的F′质粒所带动。

Plastic extrusion is kind of plastic pigmentation method that can result in comparetively stable color.

抽粒是塑料染色工艺中色泽较为稳定的塑料染色形式。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！