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Histochemical staining of immature transgenic rice seeds showed UidA gene was specifically expressed in endosperm and the highest level GUS expression was observed in aleurone layer.

对转基因植株种子的GUS 染色表明,UidA基因仅在胚乳中表达,在糊粉层中GUS表达量最高。

Histo chemical staining of immature transgenic rice seeds showed UidA gene was spe cifically expressed in endosperm and the highest level GUS expression was observ ed in aleurone layer.

对转基因植株种子的GUS 染色表明,UidA基因仅在胚乳中表达,在糊粉层中GUS表达量最高。

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株,经筛选后倒置荧光显微镜观察细胞形态及荧光状态,挑取荧光最强的克隆,命名为ES-GFP细胞,进一步扩增培养,观察细胞生长及荧光表达情况,行细胞分化全能性鉴定包括碱性磷酸酶(alkine Phosphtase,AKP)染色、体外胚胎体(embryonic body,EB)形成实验及裸鼠体内成瘤实验。2。

There is no significant difference between dark and light adaptation in the zinc distribution in bipolar, amacrine and ganglion cells.

外核层胞体锌离子染色阴性,少数散在分布的视锥细胞呈锌离子阳性。

The expression of albumin, CK-18, AAT and AFP was detected in amnion tissues and AECs cultured in vitro.

免疫化学染色显示,羊膜组织和体外传代培养的AECs中均有Alb、CK-18、AAT和AFP表达。

Methods We used retrograde CTb immunocytochemical and NADPH-d histochemical staining methods after microinjecting CTb into cortical amygdaloid.

采用霍乱毒素B亚单位逆行追踪和还原型尼克酰胺腺嘌呤二核苷酸脱氢酶组织化学双重染色相结合的方法。

If the cell density of Anabaena flos-aqua and Microcystis aeruginosa was 6×10-7×10^9 cellsl^(-1) and 4×10-5×10^8 cellsl^(-1), the FDA staining rate would be more than 94% and 91%.

对细胞密度为6×10^7-7×10^9个l^(-1)的铜绿微囊藻,FDA染色效率可达94%以上。

If the cell density of Microcystis aeruginosa and Anabaena flos-aquae was 6×10~7~7xl0~9L~-1 and 4 × l0~7~5×l0~8 L~-1, the FDA staining rate would be more than 94% and 91%.

对细胞密度6×10~7~7×10~9L~(-1)的铜绿微囊藻,FDA染色效率可达94%以上。

Wilms tumors with unfaorable histology contain anaplasia – defined as the presence of hyperchromatic, enlarged nuclei (at least 3 times as large as blastemal nuclei), and multipolar mitotic figures.

如有细胞间变则提示Wilms'瘤预后不良,所谓细胞间变,表现为染色质粗糙,胞核增大(至少是后肾胚芽细胞核的3倍大小)以及多极性有丝分裂像。

The emulsion polymerization was used to prepare the fluorine-contained resin with butyl acrylate and methacrylic hexafluoro butyl ester as monomer,so as to improve the apparent shade depth of fine-denier dyed polyester fabric.

以丙烯酸丁酯和甲基丙烯酸六氟丁酯为单体,采用乳液聚合法合成含氟树脂,用于提高涤纶染色织物的表观深度。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。