染色的

A SSR marker based linkage map covering chromosome 10 and the short arms of chromosomes 1 and 11 of rice was constructed and applied for mapping fertility-restoring genes in Zhenshan 97A/(Zhenshan 97B/Milyang 46)F_ 6 population consisting of 704 lines.

应用由704个株系组成的珍汕97A/(珍汕97B/密阳46)F6测交群体，针对水稻第10染色体和第1、11染色体短臂构建了微卫星标记连锁图谱，检测到控制野败型细胞质雄性不育育性恢复的4个QTL，其中位于第10染色体长臂中下部的Rf4具有主效效应，位于第1染色体短臂的Rf3具有较大效应，位于第10染色体长臂近着丝粒处的qRf10和第11染色体短臂近着丝粒处的qRf11表现出微效作用。

Due to the breakthrough of stained technology in 1970s, the band-pattern of chromosome provides material visual information for cytogeneticist, then analyses the composition of genome in a cell and measures the features of the equipments for karyotyping.In order to meet the demands of clinical hospital, a chromosome analysis system based on PC is developed.

由于70年代标本染色技术的突破(取代了此前的均匀染色方法，产生了染色体的带型特征)，染色体的带型模式为细胞遗传学家提供了实质性的视觉信息，从而可以进一步分析细胞的基因组的合成以及进行自动核型分类仪器中的特征测量。

To begin with, we briefly introduced the research background of the project, current research situation and development prospect of the dyeing equipments as well as factors effecting dyeing quality, in addition to the detailed analysis on mechanical structure, working principles of jigger and composition of control system, which rendered definite control objectives of the jigger control system.

本文首先简单介绍了项目研究背景、染色设备的研究现状和发展前景以及影响染色质量的因素，对卷染机的机械结构组成、工作原理和控制系统的组成进行了详细的分析，明确了智能化卷染机控制系统的控制目标。

Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型，分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本，行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况；行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润；行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达；分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

At the first time, the study of misdivision pattern of homoeologous group 5monosomic univerlants from North Chinese common wheat was conducted. The results indicated that there were 37 orietation patterns in 27cobinations including nine cultivars and three chromosomes . According to analysis of 37 orientation patterns, four misdivision patterns were discovered.

介过去的有关痔通小麦〔T.emL.lit Jt＄数分裂单体单价体错分裂的研究中，大多集中在错分裂频率及单双极分丸，而X、1上错分裂方式的研究则很小，几乎是一个被忽砚的问题'"''、1％6年R.M.rsk曾把普通小麦羊体单价体的错分裂归结为两纨包括一条染色单体的错分裂及包括两条染色单体的错分裂"。

Results (1)The permillage of myocyte apoptosis in infarct area in elder patients with sudden death caused by AMI was significantly higher than that in control group(663.00‰±117.12‰ vs 34.30‰±20.68‰,P＜0 .01).However the permillage of myocyte apoptosis in 1-vessel,2-vessel,and 3 - vessel disease were 514.28±165.12‰,564.38‰±102.33‰ and 668.25‰±127. 19‰,respectiverly,significantly higher as compared to control group(All P＜ 0.01).But no significance was found among the three groups.(2)The size of DNA f ragment about 180～200 bp was found only in those patients with two and three ve ssels involoved.(3)The electron microscope showed the characteristics of myocyte apoptosis episodes,the others showed the characteristics of necrosis.

结果 TUNEL法发现，猝死者梗死区的心肌细胞凋亡千分数老年组(663.00‰±117.12‰)明显高于正常对照(34.30‰±20.68‰)(P＜0.001)；心肌细胞凋亡千分数在冠脉1支病变者(514.28‰±165.12‰)＜2支病变者(564.38‰±102.12‰)＜3支病变者(668.25‰±127.19‰)，虽然均明显高于正常对照(均P＜0.01)，但三组之间比较则无统计学上的差别(均P＞0.05)；冠脉2～3支病变者梗死区的心肌细胞DNA电泳可见相差约180～220 bp的阶梯片段；电镜发现猝死者梗死区内的心肌细胞核膜完整、染色质浓集、电子密度增加的凋亡特征，有的则出现核膜破裂、染色质溶解成碎屑的坏死现象。

Materials and methods: The lung preparation of 6 SARS death patient (died in 9,14,20,29,33,38d) and 6 macaque model (killed in 7,12,14,14, 32, 35d)were objects. Pathological changes, collagen fibers, lattice fibers, elastic fibers, collagen I and III in lungs and fine structure changes were studied by routine H.E dyeing, trigeminy dyeing, trinitrophenol- sirius red staining and polarization microscope, electron microscope and image analysis. Expression of Vimentin、 TGFβ_1、 TNF α、 IL-1β and MMP-2 were detected by immunohistochemistry.Results:1. Pathological changes of SARS death patient.

材料与方法：以6例SARS死亡患者(分别于发病后9、14、20、29、33、38d死亡)和6只猕猴实验模型(分别于染毒后7、12、14、14、32、35d活杀取材)肺标本为对象，应用H.E染色、三联染色、苦味酸-天狼猩红-偏振光法、电镜和图像分析等技术，对比性观察SARS肺组织病理变化和纤维化的病理过程、胶原纤维、网状纤维和弹力纤维的变化特点、Ⅰ型与Ⅲ型胶原纤维的数量和分布规律，旨在探讨SARS肺纤维化的病变经历过程及特点；利用免疫组织化学和形态计量学技术检测SARS死亡患者肺脏的Vimentin、TGFβ_1、TNFα、IL-1β和MMP-2等与炎症反应和纤维化相关活性因子，探讨其发病机制。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Expression of TGF- 1 and iNOS mRNA was higher in the control chick retina and choroid than that in FDM. The immunostaining of TGF- 1 and iNOS protein mainly was detected in the outer part of photoreceptor layer, and nonspecific immunostaining was found in the RPE and choroids.

在鸡视网膜和脉络膜组织有TGF-β1和iNOS蛋白质及其mRNA的表达，TGF-β1和iNOS的阳性染色主要分布于视网膜光感受器的外节，并且位于光感受器外节的不同位置，而在RPE及脉络膜无明显的阳性染色。

Results:Methyl aldehyde can"t preserves efficiently the ultrastructure of the renal biopsies.Osmium tetroxide can preserves efficiently it but affects dyeing of the section for light microscopy.However,glutraldehyde not only preserves it better than methyl aldehyde but also doesn"t affect dyeing of the section for light microscopy.

结果：甲醛固定不能有效保存肾穿组织的超微结构，锇酸的固定能有效地保存超微结构，但严重影响了光镜切片的染色；而戊二醛与甲醛相比，较好地保存了肾穿组织的超微结构，同时又不影响光镜切片的染色。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。