染色的

There were more Ca〓 deposite granules in and around the condensing granules of nucleoplasm; 2 There were large amount of Ca〓 in the Golgi complex of spermatid in Golgi phase. The Ca〓 in the acrosomal vesicle were probably from the Golgi complex; 3 Ca〓 was probably concerned with adherence of the acrosomal vesicle to the nuclear membrane.

发现（1）。Ca〓与精子形成过程中染色质的致密化有关，圆形精子细胞局部致密化的染色质团块周围及其内部和长形精子处于的致密化过程的核内均具有较多的Ca〓；（2）。

The pigment carbon black our factory researches and produces is divided into three big series of high, middle and low, which is widely used in dyeing industry, such as plastic cement coating, the fiber staining, painting, the printing ink, the printer inked ribbon, the leather coloring, the papermaking, defferent kinds of pen industry, matching colors, mixing colors and so on, and it can be used in making scattered products such as the color cake, the color thick liquid,etc.

我厂研发生产的超细色素炭黑，分高、中、低三大系列，广泛用于塑胶涂料、纤维着色、油漆、油墨、打印机色带、皮革染色、造纸、各种笔业、配色、颜料、调色等染色业，并可用于予制色饼、色浆等予分散产品。本公司秉承"质量第一、诚信至上、周到服务、合作共赢"的宗旨，用优质的产品搭建供需桥梁、用热情服务构筑合作平台、用至诚至信密切商业伙伴，在互利互惠同谋发展的甚础上共创双方美好的明天！

Objective After Intense pulsed light irradiation cavia cobaya skin, to evaluate the histological change of skin with HE stains; to evaluate the collagen fiber change with nitroxanthic acid stains; to quantitative analysis of hydroxyproline content. Confirm mechanism of action for Intense pulsed light on sun-damaged human skin.

目的 用强脉冲光照射豚鼠背部皮肤后，通过HE染色观察皮肤组织学的改变；用苦味酸染色观察皮肤胶原纤维的改变；定量分析豚鼠皮肤羟脯氨酸的含量，观察皮肤胶原蛋白的变化，进一步研究强脉冲光治疗皮肤光老化的作用机制。

Methods①The animal model of braincontusion caused by free drop hammer was established.②The injuredtissue of rat brain were stained by TUNEL for apoptosis,immunohistochemistry for Caspase-3 and Feulgen"s for DNA. Image analysistechnique and the statistical method were employed to explorate thetemporal changes of injury time.③The DNA was extracted from ratcontusive tissue of brain and assayed by gel electrophoresis toinvestigate the relationship between the DNA fragmentation and injurytime.④One handred and seventeen cases of death from traumatic braininjury were retrospective researched to investigate the characteristicof TBI in forensic medicine.⑤The contusive tissue of human brain werestained by TUNEL, Caspase-3 immunohistochemistry and Feulgen"s methodsin the same way to analyze and disclose the linear relationship betweentemporal changes with injury time.

方法①建立大鼠自由落体打击脑损伤模型；②对不同损伤时间组的大鼠脑挫伤组织进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色，结合图像分析技术和统计学分析，探讨脑挫伤后神经细胞凋亡、DNA片段化和含量的时序性变化；③提取大鼠脑挫伤组织DNA进行琼脂糖凝胶电泳分析，观察DNA片段化随损伤时间的变化特点；④对117例颅脑损伤致死案例进行回顾性分析，探讨其法医学特点；⑤选择不同损伤时间的人脑挫伤组织同样进行TUNEL、Caspase-3免疫组化、Feulgen's DNA染色和分析，观察上述指标与损伤时间的关系。

In double labell the high contrast substrates of enzym should be used.

因而作者认为，在进行胰岛内分泌细胞的双重免疫组化染色时，一染后可经PBS漂洗后直接进行第二种抗原的免疫组化染色，既节省时间，又减少了因不同的化学试剂的应用而影响第二种抗原活性的可能性。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以Con A作为T细胞活化、增殖的刺激剂，以不同浓度的TMB-8及与环孢菌素A联合作用于该系统，用流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术，分析TMB-8在Con A刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色分析细胞周期的分布情况。

Silenced chromatin, largely heterochromatic,contains a number of restrictive histone modifications, such as meH3K9, meH3K27, meH4K20,and histone deacetylation, which allow for a higher-order packing and inaccessibility to transcription factors.

沉默的染色质，大部分的是异染色质，包含大量的限制性的组蛋白修饰因子，例如meH3K9， meH3K27， meH4K20和组蛋白的脱乙酰作用，这些因子需要高度包装和不易转录因子。

After the culture under the mineralization fluid condition in about 1 wk, the compact circular group appeared in the intercellular space, simultaneously cell multiplication ability reduced compared with the conventional culture; The sodium alizarinsulfonate dyeing showed the compact and circular lightproof briquetting brown laminated shape in the mineralizd intercellular spac, I collagen immunity group dyeing was strongly positive in the coloring region.

在矿化液条件下培养1周左右，细胞间出现了致密圆形团，同时细胞的增殖能力较常规培养有所降低；茜素红染色结果显示矿化细胞间出现的致密的、圆形不透光团块呈现片状的棕染，着色区域， I型胶原的免疫组化染色强阳性。

During the surgery, the lesion was found to be a solid tumor. A surgical wedge biopsy was done and purulent dark yellow fluid was drained from the center of the mass. Histologically, there was a solid pattern of rhabdoid and anaplastic round or oval cells haphazardly arranged with poor differentiation. Immunohistochemical staining was positive for vimentin, a mesenchymal marker; the reticulin staining was consistent with carcinoma.

我们实施了手术切片及肿瘤中心脓状物引流，病理组织学上，我们发现了不规则或未分化的横纹状、圆形及椭圆形细胞；免疫组织染色上，细胞广泛的对非上皮标记vimentin呈阳性反应、且其reticulin染色呈现出腺癌的形式；肉瘤及腺癌的特色皆可在切片中看到，所以我们的诊断是类肉瘤性线癌。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。