染色的

Our results demonstrate: The structures of the organs are normal, and the shapes of cells are clearly visible. There are lots of positive brown granules in Chief cells and Parietal cells in abomasum as well as the mucosa epithelial cells and gland cells of duodenum. Three bands with a molecular mass close to 120KDa、110KD and 98KDa were identified by Western Blot. The Ob-R levels of 120KDa in abomasum were significantly higher than that of in small intestine. The levels of 110KDa were similar in the two organs. The expression of 98KDa Ob-R was weak.

HE染色结果显示，各组织结构正常，细胞形态清晰可见；免疫组织化学SABC染色显示，在皱胃胃体部固有层胃底腺的主细胞和壁细胞及十二指肠黏膜上皮细胞和固有层肠腺的柱状细胞中均可见大小数量不等的棕黄色颗粒；western Blot 实验发现，在胃和小肠均检测到120KDa、110KDa和98KDa三条带。120KDa长型瘦素受体蛋白在胃中表达量显著高于小肠中的表达；110KDa的短型瘦素受体蛋白，在小肠和皱胃中表达量接近。98KDa短型受体蛋白在胃和小肠表达均较弱。

There is an opinion that majority of patients with IND can be treated successfully with conservative treatment such as diet, laxatives and prokinetic drugs, however, in some severe cases a transient enterostomy or a segmental resection is unavoidable.

IND的诊断标准仍存在争议，Meier-Ruge提出了改良的粘膜活检来诊断IND，但行多处肠壁的全层病检，HE染色或Ache染色，对神经元发育不良较难判断的，加行神经元相关免疫组化检测的方法较为公认。

Since then, the gene localization has developed from simply being described as at the euchromatin region and heterochromatin region to more complicated nuclear localization. Therefore, the gene regulation attracted more attention now.

因此基因在细胞核内的定位就由原来简单认为的异染色质区和常染色质区发展得更为深入了，基因在细胞核内的定位以及位置的变化与基因表达调控之间的关系也越来越受到重视。

Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶，比活性达130.9 U／mg，纯化倍数为137.4倍；纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰，且两者完全重叠；进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色，各在负极端出现一个活性斑点和蛋白染色斑点，且两者的位置完全对应；表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

The typical apoptotic morphological features appeared in MUTZ1 cells treated with 4 mmol/L VPA for 72 hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dosedependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dosedependent manner, the cells were arrested at G0/G1 phase.

结果显示： VPA对MUTZ1细胞的生长抑制作用呈现时间和剂量依赖性；经4 mmol/L VPA处理MUTZ1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞浆内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

The typical apoptotic morphological features appeared in MUTZ-1 cells treated with 4mmol/L VPA for 72hours. Pyknosis of cells and nuclei, disintegration of nuclear chromatin and apoptotic body could be observed by light microscopy. Aggregation and margination of nuclear chromatin, concentration of plasm, increment of density and chromatin mass of irregular size could be observed by transmission electronmicroscope. The flow cytometric analysis indicated that the VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner, ratio of cells at G0/G1 phase increased and ratio of cells at S phase decreased in dose-dependent manner, the cells were arrested at G0/G1 phase.

结果显示：VPA对MUTZ-1细胞的生长抑制作用呈现时间和剂量依赖性；经4mmol/LVPA处理MUTZ-1细胞72小时后，细胞呈现典型的凋亡形态特征，光学显微镜下可见凋亡细胞胞体固缩、核固缩、核碎裂及凋亡小体；透射电子显微镜下可见凋亡细胞核染色质边集、胞浆浓缩、密度增加，胞装内大小不规则的染色质团块；流式细胞术结果表明，细胞凋亡率随着VPA浓度的增加而逐步增高，G0/G1期细胞比例随着VPA浓度的增加而逐渐增多，S期细胞比例逐渐减低，细胞被阻滞在G0/G1期。

In quality management, technical testing laboratory with advanced computer color measurement instrument and automatic batching machine, drip feeder to ensure that the staining formula is reasonable and accuracy, with 15 computer-controlled high temperature and high pressure dyeing small prototype can completely simulate the actual production, thereby ensuring that the character color.

在质量管理方面，技术检测化验中心拥有先进的计算机测色仪和自动配料机，滴料机以确保染色配方的合理性和精确性，拥有15台计算机控制的高温高压染色小样机可完全模拟实际生产，从而确保色纱的品性。

The purpose of the research was to obtain bacterial polyhydroxyalkanoic acids producers with good characters. With bottom sludge from Donghu Lake, bum soil from hillside, fertile vegetable soil and oil-soaked soil as initial samples, the bacterial strains with ability of synthesizing PHAs were screened not through enriching, isolation and purification, staining with Sudan black B and qualitative and quantitative analyses by infrared spectroscopy and gas chromatography. The results of staining with Sudan black B showed that there were 3 strains with ability of synthesizing PHAs among the 12 isolated strains and their numbers were Zj-5, Zj-8 and Zj-9 resp. All the 3 strains took on adsorption peaks at the points near 1741 cm^(-1) and the infrared spectrums of the strains Zj-8 and Zj-9 were similar.

［目的］获取性状优良的聚羟基烷酸合成菌［方法］以东湖底混、山坡裸地土、肥沃菜园土和油脂浸润土为初始样品，经富集、分离纯化、苏丹黑B染色、红外光谱及气相色谱定性定量分析筛选出具PHAs合成能力的细菌菌株［结果］苏丹黑B染色结果表明，在分离出的12株细菌中有3株细菌具有PHAs合成能力，其编号分别是Zj-5、Zj-8和Zj-9.3株细菌均在174cm^(-1)附近呈现吸收峰，菌株Zj-8和Zj-9的红外谱图相似。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

In adjacent sections, ALP staining was used to classify BMUs as quiescent or actively forming bone.The areal densities of scl- and scl+ osteocytes in the BMU were inversely correlated and were strong determinants of ALP status in the BMU.

在邻近切片，用ALP染色把BMUs分为休眠或活跃骨形成，这个BMU内区域的scl-和scl+骨细胞的密度呈负相关并且是这个BMU内ALP染色状况的有力的决定因素。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。