染色的

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Moreover, all the signals on X and Y only located in the heterochromatic regions, showing the same distribution with those of C-band.

所有X染色体上的信号是一致的，而X、Y染色体上的信号分布则彼此不同。X、Y染色体上的杂交信号区域均集中于异染色质区，信号分布模式同X、Y上异染色质分布模式完全吻合。

There were 8 patients in the macromastia group and 5 patients in the control group. The result didn't show significant difference between the two groups. There is no difference between the two groups in mammary epithelial cell size measurement and Akt-1/2/3 immunohistochemistry stain. The levels of Ki-67, phospho-Akt-1/2/3, and STAT5a of juvenile macromastic patients are higher than those of the patients of the control group, and the results are statistically significant.

三年内共搜集到八位巨乳症病患及五位对照组，两组以Wilcoxan rank sum test比较，乳腺上皮细胞的尺寸及Akt-1/2/3免疫组织化学染色方面在统计学上的没有显著的差异，而乳腺上皮细胞之Ki-67、phospho-Akt-1/2/3、及STAT5a免疫组织化学染色方面，则均为青春期巨乳症患者组较高，统计学上均有显著性的差异。

Methods The skeletal muscle specimens with obviously regenerating muscle fibers were obtained from 9 patients with pseudohypertrophic muscular dystrophy and 5 patients with polymyositis. Then frozen serial sections were made and the sections were treated with HE stain, immunohistochemistry stains including antiEzrin and antiNeural cell adhesion molecule monoclonal antibodies. The pathological changes and the expression of Ezrin were observed.

取肌纤维再生活跃的假肥大型肌营养不良（DMD，9例）和多发性肌炎（PM，5例）患者的骨骼肌标本，冰冻连续切片，进行HE染色及抗Ezrin、抗神经细胞黏附分子单克隆抗体免疫组化染色，观察被检肌的病理改变和Ezrin蛋白的表达。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现，次生壁形成早期，细胞核具有双层核膜，染色质凝聚，可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中；随后，双层核膜消失，细胞器将逐渐解体，多泡体开始出现在纤维细胞的细胞质；随着年龄的增加，纤维细胞壁逐渐增厚，并出现多层结构现象，而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

Methods KC and FE of tympanic membrane were cultured on the surface of CCM prepared by torrefaction, then the cells biological characteristics were detected by immunofluorescence stain of keratin and vimentin, and the cells proliferative activity were identified by immunofluorescence stain of PCNA.

方法热干法制备CCM，传代的大鼠鼓膜上皮KC和FB分别接种于培养板及CCM表面，通过角蛋白、细胞膜波形蛋白免疫荧光染色鉴定细胞特异性，通过细胞核增殖抗原(proliferating Cell nuclear antigen， PCNA)免疫荧光染色测定膜上细胞的增殖能力，通过细胞培养液上清的经脯氨酸测定评价对FB胶原分泌功能的影响。

Culture and immunohistochemistry of Schwann cells Schwann cells that are derived from sciatic nerve of Sprague-Dauley rats are successfully isolated, cultured. The Schwann cells are transplanted into the hemisected adult rat spinal cord after purification and condense and enclosed by L I -antibody. Eight weeks later, the experiments of the S- 100 immunohistochemistry indicate that Schwann cells are still alive and array orderly relatively.

本实验的主要研究内容及结果如下： 1、雪旺细胞的培养及免疫组化染色利用新生S-D大鼠的坐骨神经成功地分离、培养获得了纯度较高的雪旺细胞，并将雪旺细胞移植进入大鼠脊髓损伤创面，饲养8周后经过S-100免疫组化ABC染色发现雪旺细胞在大鼠体内成活良好并成簇排列。

FCM appeared obvious inferior diploid peak and blocked the cells to the G_1 phase.The red particles throughout the cytoplasm and permeated the plasma membrane could also be seen.Chromatin lining and breeding body could be seen under the transmission electron microscope.

FCM出现明显的亚二倍体峰并将细胞阻滞于G1期，Annexin-V-FITC联合法染色可见细胞凋亡的特征性贯穿胞质穿过胞膜的红色颗粒，电镜下可见染色质边集及生发小体的凋亡形态特征。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

Hontonpur H is a high efficient environmental friendly detergent for dye box, which has great melting and dispersing ability on the attached dyes, dye resolvent, polyester multi-polymer and other multi-compounds. It also has excellent effect on remove inflexible dirt like metal salts. Process shorten and cost saving.

Hontonpur H 是一种高效染色机用环保型洗净剂，它对于染色机内墙上附着的染料、染料分解物及聚脂纤维齐聚物等复合凝聚物具有良好的溶化分散性能，对去除变成金属盐的顽固污垢可发挥优异的效果，并可缩短工艺，节约水电。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。