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METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞,运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化;用gp120处理并用anti-gp120-FITC进行染色,运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况;用抗磷酸化ERK 抗体免疫荧光方法进行染色,运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

Methods The AGS cellseeded coverslips were prepared, then the two different status of Lactobacillus acidophilus and two different forms of Hp were added in different order. 2 hours later, the bacteria were Garm's stained, and the adherence of Hp to AGS cell were observed by microscope and the adherence index was calculated.

制备人胃上皮腺癌细胞系细胞爬片,将两种状态嗜酸乳杆菌和不同形态Hp菌分组按不同顺序加入,作用2 h,革兰染色后光镜观察分析Hp黏附指数产生的变化,荧光抗体染色法定性评价黏附于AGS细胞的Hp密度的改变。

Incubated with cytochalisin B for 4 hours, F-actin bunddles disrupted, confluent ECs monolayer has a complete detachment from the coverslips. So, there is a suggestion that the F-actin in ECs is essential for endothelial adherence under shear stress.

Northcrn杂交结果表明外源性纤维连接蛋白有促进EC肌动蛋白表达的作用,结合F-actin染色及FN受体染色结果,本文认为用纤维连接蛋白作为细胞种植的促粘附物质有助于内皮细胞粘附结构的形成。

Chromodomain is a conserved gene element and has been found in Eukaryon from yeast to mammal. It is involved in chromatin remodling and gene expression regulation.

染色质域是从酵母到哺乳动物的真核生物中普遍存在的一个保守的基因元件,参与染色质重组和基因表达调节。

The results show that dye molecular structure is the decisivefactor in dye fastness. The dye with polar groups or having large molecularweight has good fastnesses to light and ironing.

试验结果表明,染料分子结构是影响染色牢度的决定性因素,在染料分子中具有极性基团或具有较大分子量的染料,其染色产品具有较好的耐日晒牢度和耐熨烫牢度。

It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion-induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions.

采用硅管套接大鼠切断的坐骨神经模型,局部给予胶质细胞源性神经营养因子,应用尼氏染色、酶组织化学染色方法,观察到外源性GDNF能减少脊髓修复侧前角运动神经元死亡的数目,降低脊髓前角运动神经元及脊神经节感觉神经元中胆碱酯酶及酸性磷酸酶变化的幅度。

Methods: Cells isolated from neonatal rat long bones attached to matrix or sedimented onto bone slices and were stained with Gimsa and for tartrate-resistant acid phosphatase .

从新生SD大鼠的股骨、胫骨和肱骨的骨髓腔面分离粘附性强的细胞,直接在倒置显微镜下观察其活体形态或者经Gimsa染色后观察其形态;用抗酒石酸酸性磷酸酶染色对其进行鉴定。

The Chinese white poplar veneer and birch veneer were dyed with different color fastening methods following an orthogonal experiment so as to investigate the effects of different dyestuffs,color fastening agents,dosages and add in methods of color fastening agents on the color stability of the dyed veneer.

设计正交试验对大青杨单板和桦木单板进行不同固色工艺的染色处理,考察不同的染料、固色剂、固色剂添加量以及添加方式对染色单板颜色稳定性的影响。

Is a printing and dyeing Caps Interlining manufacturers, the introduction of advanced automation and finishing equipment with higher levels of the three production lines, annual production capacity of 50 million meters, the main product real wax printed cloth, imitation wax calico, reactive printed cloth, crafts and various±special interlining cloth dyeing bleaching processing.

昌邑源祥印染有限公司是纺织品染色印花及工艺帽衬布的生产厂家,引进了先进的自动化程度较高的染整设备生产线三条,年生产能力 5000 万米,主要产品真蜡印花布、仿蜡印花布、活性印花布、工艺运动帽专用衬布及各种染色漂白布加工。

This paper sums up the achievement of domestic and foreign researches on the dyeing strength of the vegetable dye,and probes into the factors influencing the sun-shining strength ,washing strength and rubbing strength of the vegetable dye.

总结了国内外在植物染料染色牢度方面的研究成果,探讨了影响植物染料染色织物的耐光牢度、水洗牢度和摩擦牢度的因素。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。