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Furthermore, K562 cells transfected by lentivirus plasmids were induced into the erythropoietic cells and the erythropoietic differentiation of K562 cells were examined by benzidine staining, immunocytochemistry and RT-PCR.

进一步对SOCS-3基因沉默后的K562细胞进行了诱导分化,并采用联苯胺染色法检测K562细胞向红系分化比例变化,免疫荧光染色检测细胞表面抗原的变化,RT-PCR 检测造血相关基因的变化。

HepG2 cells were exposured to oxymatrine, final concentrations of which were 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 1, 2, 5, 8, 10, 15 g.L-1 respectively, MTT method was used to determine the inhibiting effects of oxymatrine on HepG2 cells proliferation, followed by Haematoxylin and Eosin staining to observe the cell morphology.

以0.1,0.2,0.3,0.4,0.5,0.8,1,2,5,8,10,15 g.L-1的氧化苦参碱处理HepG2细胞,四噻唑蓝法检测其对细胞增殖的影响;苏木精-伊红染色比较细胞形态差异;免疫组化染色进一步检测HepG2细胞肿瘤相关蛋白表达量的变化。

CPGES mRNA signal was maily located in oocyte cytoplasm and declined at 48 hafter PMSG treatment.

在各级卵泡的卵母细胞质中有很强的cPGES免疫染色,仅在PMSG注射48小时,在卵母细胞质中的cPGES免疫染色有所下降。

Because more than 75. 4% regions were occupied by heterochromatin on the sex chromosomes of spiny eel, its X library should be a good tool to isolate the heterochromatic highly repetitive sequences DNA and study its origin and evolution.

在刺鳅的性染色体上,75.4%以上的长度都是异染色质区域,因此,刺鳅X染色体文库也为分离获得鱼类性染色体上异染色质重复序列,探讨其起源和进化规律提供了良好的实验材料。

The origin of the heterochromatic regions on the long arm of maize B chromosome was studied in two ways: the Southern signals of the CL-repeat; and its deletion, insertion, and mutation variants.

本研究分别以CL-repeat的南方杂合讯号以及因缺失、插入和突变造成的序列变异两个角度,来探讨玉米B染色体长臂远端异染色质(distal heterochromatin, DH)区的起源。

The origin of the heterochromatic regions on the long arm of maize B chromosome was studied in two ways: the Southern signals of the CL-repeat; and its deletion, insertion, and mutation variants.

中文摘要本研究分别以CL-repeat的南方杂合讯号以及因缺失、插入和突变造成的序列变异两个角度,来探讨玉米B染色体长臂远端异染色质(distal heterochromatin, DH)区的起源。

[Objective]To explore the effect of continuous physiological osteogenic stress on the bone healing of canine iliopectineal crest.[Method]Ten adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum,which were fixed with ATMFS or steel plate respectively. The animals were sacrificed and specimens were procured at 2,4,6,8 and 12 weeks after operation.Samples from the fracture gaps were investigated by histology of HE and Masson staining,image pattern analysis of new bone formation.

探讨持续顺应生理力线的压应力对犬骨盆弓状线部骨折愈合的影响[方法]选用10只成年杂家犬,双侧髋臼臼顶上方1.5 cm处横形截骨,分别采用ATMFS(骨盆髋臼三维记忆内固定系统)柱固定器和5孔重建钢板内固定,于术后2、4、6、8、12周处死动物取材,行HE染色、Masson三色法染色组织学检查,图像分析新骨形成积分光密度值,研究不同应力作用模式下的骨盆骨折愈合情况。

Results Four weeks after surgery, animals in DO group showed an increased voiding frequency, higher voiding pressure and irregular nonvoiding contractions (n=12, P.05). We found increasing mutual connection and multilayer construction in suburothelium and intermuscular layers of DO but not DS and control bladders.

对照组、DS组和DO组豚鼠膀胱均观察到ckit染色阳性且呈典型长梭形有突起的ICC,主要分布于平滑肌肌束间和黏膜下层;在DO组豚鼠的膀胱中ckit染色阳性的ICC分支突起增加且互相连接呈网络状,而在对照组和DS组中ICC呈散在分布,细胞之间联系较松散。

Furthermore, heterochromatinization should be an earlier event than pericentric inversion.

另外,从刺鳅的X、Y上结构异染色质的相似分布来看,在性染色体进化过程中,异染色质化发生的时间应该早于臂间倒位。

EP2 immunostaining was gradually strengthened in the luminal epithelium and glandular epithelium from day 1 to 5 of pregnancy, followed by the gradual reducing of immunostaining on days 6-9. No specific signals for EP2 mRNA were detected in the uterus under delayed implantation, but strong signals were present in the luminal epithelium after reinitiation of implantation.

妊娠第1-5天的腔上皮和腺上皮上EP2免疫染色逐渐增强,到第6天时子宫腔上皮和腺上皮上有强的免疫染色,在随后妊娠第7-9天的子宫腔上皮上逐渐降低。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。