染色的

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

Results In define induction envioroment, HPMSCS can diferentiate into lipoblast、after 2 weeks, the induced cells are full of lipid droplet;HPMSCS can differentiate into osteoblasts, after 4 weeks, we can see calcified tubercle in the cells, calcium in the calcified tubercle can be dyed black by Von Kossa and ALP dying is positive reaction above 95%.

结果 HPMSCS在培养基中持续培养后，在特定诱导环境下，成功使其向成脂肪细胞转化，培养2周时，可见融合成团的脂滴充满整个细胞；成功使其向成骨细胞转化，培养4周以上可见明显钙化结节，von Kossa染色可将骨结节中沉积的钙染成黑色，碱性磷酸酶染色呈强阳性反应达到95%以上。

The cartilage histological characteristics were observed according to the method of Mankin. Immunohistochemical staining was performed to investigate the expression of VEGF.

按照Hulth法建立膝骨关节炎模型，术后第5周起至第7周末，阳和汤组予混有阳和汤的颗粒饲料，正常组及模型组均给予普通饲料；术后第8周分别取各组胫骨平台关节软骨，采用番红O染色，进行Mankin评分；免疫组织化学染色测定关节软骨中VEGF表达的阳性指数。

All rats were sacrificed at 12 weeks after operation. the macro-pathological changes of samples were graded by mankin's scale.bone mineral density of right distal femoras and femora condyles were measured by dual-energy x-ray absorptiometry scanner.

术后12周处死动物取材，采用mankin评分系统评分；用双能骨密度仪测量右侧股骨远端1/4骨密度和股骨内外侧髁软骨下骨的骨密度；右侧股骨髁脱钙后切片，行番红&o&染色和mmp-13免疫组化染色；右侧胫骨近端做硬组织切片，测量软骨下松质骨的骨组织形态计量学参数。

Morphologically, the macrophages treated with NO and O-LDL presented shrinkage, nucleus fragmentation, and cytoplasm condensation; DNA ladder was visualized by agarose gel electrophoresis; Characteristic apoptosis peak of macrophages was showed by flow cytometry; Ultrastructure, The chromatin condensed and marginated to form dense chromatin fragmentation; The cytoplasm concentrated and vacuolized; The lysosomes and mitochondria of apoptosis macrophages increased clearly.

电镜观察超微结构变化：染色质固缩边聚，形成致密、均质的染色质块，胞质浓缩、电子密度升高并空泡化；凋亡巨噬细胞溶酶体、线粒体增多；细胞突起缩短减少，但巨噬细胞特有的板状伪足不消失。

Objective To evaluate the frequency of microdeletions in the long arm of Y chromosome of idiopathic infertile males with azoospermia and oligospermia in Xinjiang province in China and to investigate the difference of Y microdeletion frequencies between two nations.

目的评估新疆地区汉族、维吾尔族不明原因无精子症和严重少精子症男性患者Y染色体长臂微缺失的频率，探讨不同民族间Y染色体长臂微缺失发生率的差异。

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

Histological changes of periostea on the mandibular ramus after the periosteal distraction:2.1 20 adult New Zealand rabbits were operated and distracted with the same methods mentioned above.2.2 Every 5 animals were killed postoperatively at days 28,35,42 and 56 respectively.

2.2、术后第28、35、42和56天分别处死5只实验兔，每组随机选其中2只取实验侧和对照侧的下颌升支骨膜进行HE染色观察；每组3只实验兔取实验侧和对照侧的下颌升支骨膜进行增殖细胞核抗原免疫组织化学染色观察。

And Sirius Red in a supersaturated picric acid solution viewed by polarization microscopy. The changes of mRNA expression of collagen type I were analyzed by RT-PCR. The results were compared with those after intervention with Losartan.

染色及苦味酸天狼猩红胶原特异染色偏振光显像进行心脏间质胶原的组织化学观察，RT-PCR观察心脏I型胶原mRNA表达的变化，并与氯沙坦干预后比较。

Result: Porosis and inflammatory cell infiltration in the spinal cord, rupture of muscle bundles, hypertrophia and over growth of muscle cells and collagens, and inflammatory cell infiltration in the bladder wall could been seen under HE dyeing. Nissal dyeing showed the decrease of neurons at the injured segment, upper and lower segments of the spinal cord. Result of immunohistochemistry showed there were caspase-3 positive expression in all the segments. The injured spinal cord and bladder expressed significantly more caspase-3 positive results (P.0l), upper and lower segments were lower than the injured segments (P.01), but higher than the control team (P.0l).

结果：HE染色发现脊髓损伤后脊髓内空洞形成、炎性细胞浸润等病理学变化，膀胱壁中可见到肌束断裂、肌细胞肥大增生、胶原增生、炎性细胞浸润等病理学改变；Nissal染色显示脊髓损伤后损伤节段及其上、下节段均出现神经元数量的减少；免疫组化结果表明3组均有不同程度的caspase-3表达，损伤组脊髓和膀胱内膜caspase-3表达较正常组显著增加(P.01)，损伤上段及下段caspase-3表达较损伤节段为少(P.01)，但均高于正常对照组(P.01)。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。