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The alveolitis and pulmonary fibrosis were examined by HE staining. The serum HYP was examined .The expression of CTGF was tested by immunohistochemical assay.

HE染色检测肺组织肺泡炎及肺纤维化程度;测定血清羟脯氨酸的含量;免疫组化染色检测CTGF的表达。

Institute of Genetics,Fudan University,ShanghaiThe C-banding and BrdUrd-Hoechst-Giemsa replication pattern in somatic chromosomes in Bufo bufo gargarizans and sister chromatid differential staining technique of the somatic cells in Amphibia are presented in this article.

本文报道了中华大蟾蜍的C带和BrdUrd-Hoechst-Giemsa复制带,并以中华大蟾蜍为代表确立了两栖类体细胞的姐妹染色单体差别染色方法。

Methods Rats were exposed to S band HPM which average power densities were respectively 2~90 mW/cm^2. HE staining, Toluidine blue staining and electron telescope were used to study the change of the structure of cortex, and high performance liquid chromatography was used to study the changes of 4 amino acids (glutaminic acid, asparagic acid, GABA and glycine) of cortex in 6 h and 1d after exposure to 10 and 50mW/cm^2 HPM.

采用2~90mW/平方公分S波段高功率微波辐照Wistar大鼠,通过苏素素-伊红染色、甲苯胺蓝染色和电镜观察大脑皮层的形态结构改变;采用高效液相色谱仪检测10和50mW/平方公分照后6h和ld大脑皮层中4种氨基酸(谷氨酸、天门冬氨酸、γ-氨基丁酸和甘氨酸)含量的变化。

A special staining method for the simultanouse demonstration of nodal cell, myordiac cell, collagenic and elastric fibers was applied on a same sheet of glass. This technique is simple and dired. There is contrast sharply with color.

本文建立了一种研究心脏传导系统和心血管形态学的特殊染色技术(改良的Masson's三色法十Verhoeff's弹力染色法),能在同一玻片上将起搏细胞、工作心肌、胶原纤维及弹力纤维区分开来,且操作简捷,色彩对比鲜明。

AIM: To investigate the effect of PD98059, a specific repressor of extracellular signal regulated kinase1/2 (ERK1/2), on the proliferation of the murine lymphocytes. METHODS: Lymphocytes were isolated from lymphoid nodes of BALB/c mouse. After stained with carboxy fluorescein diacetate, succinimidyl ester, the cells were stimulated with polyclonal activators, concanavalin and phorbol 12,13 dibutyrate plus ionomycin.

分离小鼠淋巴结细胞,藉羧基荧光素乙酰乙酸染色,用多克隆刺激剂刀豆A蛋白或者佛波醇酯加离子霉素刺激,以流式细胞术分析PD98059对淋巴细胞增殖的影响;利用碘化丙锭染色分析PD98059对细胞周期的影响。

Strong level of EP3 immunostaining and moderate level of EP4 immunostaining were detected in endometrial vascular endothelial cells on days 0 and 6 of the estrous cycle.

子宫血管内皮中,在发情期和发情周期第6天,检测到中等强度的EP4免疫染色和很强的EP3免疫染色。

Methods: Combined with the morphological character of the gangliocyte in the intestinal wall under microscope, the stained result and distributed character of the diseased intestinal segment in clinical and pathological diagnosed as congenital macrocolon was stained and compared with S-100 and NSF and with the immunohistochemistrical technique.

结合光镜下肠壁神经节细胞的形态特征,并应用免疫组化技术对25例临床及病理诊断为先天性巨结肠的病变肠段行S-100和NSE染色,比较染色结果和分布特点。

Methods:5Fliorouracil was added to culture medium of BGC823 gastric cancer cells and continuously cultured for 5h or 10h,then the human gastric cancer cells were stained with haematoxylin Eosin、dyed with immunohistochemical method and acid Ulead citrate,which was observed under light and electiom microscope.

目的:探讨5氟尿嘧啶对人胃癌细胞的结构和GSTπ表达的影响。方法:在人胃癌BGC823细胞株培养液中加入5氟尿嘧啶,继续培养5h或10h,然后用HE、免疫组化染色和铀铅染色。

In this research, we made use of classical histochemical methods and simple immunohistochemical methods.

本论文完全利用传统的组织化学染色和简单的免疫组织化学染色来进行研究及评估。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富,软骨基质染色均匀,软骨细胞数目最多,Ⅱ型胶原蛋白表达最活跃,平均积分光密度值最高;青少年组软骨膜内血管减少,软骨基质染色出现明显的不均质状,软骨陷窝体积变大,并呈分隔状,陷窝内软骨细胞数目减少,II型胶原蛋白表达较儿童组减弱;成人组软骨膜血管、细胞成分明显减少,软骨膜内的纤维成分明显玻璃样变,钙盐沉积较青少年组时明显增多,Ⅱ型胶原蛋白表达较青少年组减弱。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。