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ResultsA slide of bacterial suspension with a concentration of 1.5×1012/L , to be placed at 37 ℃ for 10 min then stained by carbolfuchsin for 10-15 min. Under a microscope, a clear background with red thalli and faint red flagellum could be seen in 80%-90% of the bacteria.

结果 浓度为1.5×1012/L的菌悬液37 ℃放置10 min制片,用石炭酸复红法染色10~15 min,水洗,干燥镜检,其背景清晰,80%~90%的细菌有鞭毛,菌体红色,鞭毛淡红色,染色效果理想。

Chromosome condensation is a significant process of cell multiplication and occurs as a prelude to sister chromatid segregation that initiates at the onset of anaphase.

在细胞增殖过程中,染色质集缩是细胞分裂得以正常进行的重要条件,也是后期姊妹染色单体分离的序曲。

Sural nerve biopsy showed amorphous eosinopbilic material on HE staining, which appeared positive on cogon red staining in the endoneurium around capillaries.

腓肠神经活检显示有髓纤维几乎全部消失,HE染色可见神经内膜毛细血管周围均匀粉染的物质,刚果红染色阳性,电镜下为直径7—10nm不分支的细丝。

Chromosome segregation and cytokinesis must be tightly coordinated to ensure that chromosomes are accurately partitioned between dividing cells.

染色质隔离和胞质分裂必需紧密地协调,从而确保染色质在分开的细胞间准确的分离。

In the normal group, electron microscopy showed normal chondrocytes were oval, and cells and cell membrane were intact. In cytoplasm, there were abundant rough endoplasmic reticulum, dictyosome, and mitochondria with intact nucleus, and even chromatin. In the model group, chondrocytes exhibited obvious pyknosis and their shape was irregular. The areolae around cells disappeared; cell organs in cytoplasm coagulated to flaps which showed electron dense and was uneasy to observe; the shape of nucleus showed irregularity, and caryotin gathered densely.

电镜下正常对照组软骨细胞呈椭圆形,细胞及胞膜完整,包质内可见丰富的粗面内质网、高尔基体、线粒体,细胞核完整,染色质分布均匀;模型对照组软骨细胞明显固缩且外形不规则,细胞周晕消失,胞浆内细胞器凝成高电子密度的片状物不易分辨,细胞核不规则,染色质浓聚,散裂于核中。

Because of the limitation of experimental condition, it was hard to find the primer for distinguishment between 9311 and 0293.(4) It had a good result to get perfect banes under the condition of electrephoresis with 100-120 volt/cm and 2.5EB staining.

实验凝胶电泳和染色采用2%的琼脂凝胶100-120伏八m电压电泳,随后进行2.5%EB染色,可以显示出良好的扩增产物带型。

Carotid artery samples of control and Doxy treatment groups underwenthistological and morphometric analyses at 1,3,7,14,28 days after injury respectivly, and wemeasured the thickness and area of neointimal, lumen area and the proliferation of SMCsusing a computer-assisted morphometric analyses system.

于术后 24h、3d、7d、14d、28d 原位灌注固定颈动脉后取标本,采用 HE 染色、VVG 染色、免疫组化标记 a-actin 和增殖细胞核抗原以及透射电镜的方法观察损伤动脉内膜厚度、管腔重构及 SMC 增殖的情况。

Durante M,Frusawa Y,George K,et al.Rejoining and misrejoining of radiation-induced chromatin breaks.

然而,G2期等点染色单体断裂的绝对增加值远少于染色单体断裂的绝对增加值。

Results There were significant differences between MDS and CAA in Hb, red cell distribution width-coefficient variation, immature reticulocyte fraction, BPC, the ratio of G1 (the sum percentage of myeloblast and premyelocyte) to G2 (the sum percentage of neutrophilic myelocyte and metamy-elocyte), the ratio of E1 (the sum percentage of proerythroblast and early erythroblast) to E2 (the sum percentage of intermediate erythroblast and late erythroblast), megakaryocyte count,erythroblast PAS, neutrophil alkaline phosphatase, and serous levels of indirect bilirubin,lactose dehydrogenase, folic acid, VitBl2 and ferritin.

结果 MDS患者血红蛋白,红细胞体积分布宽度。变异系数、未成熟网织细胞比率、血小板计数、骨髓原始细胞及早幼粒细胞之和与中性中幼粒细胞及中性晚幼粒细胞之和的比值、原始红细胞及早幼红细胞之和与中幼红细胞及晚幼红细胞之和的比值、巨核细胞计数、有核红细胞糖原染色阳性率和阳性指数、中性粒细胞碱性磷酸酶染色阳性率和阳性指数、血清间接胆红素、乳酸脱氢酶、尿酸、叶酸、维生素B12(VitB12)、铁蛋白水平等常规实验室指标与CAA患者比较差异有统计学意义。

The results shows, drown or etherization to make mouse death, Alcohol-fixed, defatted with 3% potassium dichromate, vitrification with Potassium hydroxide, coloration of bone with Alizarin Red S, the glycerol-ammonia were respectively coloration of cartilage with Alcian blue, vitrification with glycerol which had different concentrations, were the best conditions for making transparent specimen.

结果表明,水淹或乙醚麻醉处死、酒精固定、3%重铬酸钾脱脂、氢氧化钾透明、茜素红硬骨染色、阿利新蓝软骨染色、甘油-氨水漂白、甘油逐级透明的制作方法,获得的透明标本效果最佳。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。