染色的

P1 BMSCs, P3 CCs, and P3 ADAS were implanted into collagen/hyaluronic acid scaffold at different concentrations of 10%, 20%, 50%, 80%, and 100%, and the cells were then three-dimensionally cultured in serum-free culture media. Two weeks later, SO staining and immunohistochemical staining were employed to observe the synthesis between of chondroitin sulfate and type II collagen.

取P1骨髓间充质干细胞，P3软骨细胞和P3脂肪源性成熟基质细胞种植入不同比例(10%，20%，50%，80%，100%)的胶原/透明质酸支架中，在无血清培养液中三维培养。2周后，用SO染色和免疫组织化学染色分析，观察硫酸软骨素和Ⅱ型胶原合成情况。

This article has four chapters,which mainly study totalcoloring and adjacent vertex distinguishing total coloirng of graphs.

本文分为四章，主要研究了图的全染色及邻点可区别全染色。

But if the cell density of Anabaena flos-aquae increased to 5×10^9 cellsl^(-1), the staining rate would fall to 67% obviously due to cell twisting together.

对细胞密度为4×10^7-5×10^8个l^(-1)的水华鱼腥藻，FDA染色效率可达91%以上，但细胞密度增大到5×10^9个l^(-1)时，由於藻丝体易卷曲在一起，FDA染色率下降到67%。

But if the cell density of Anabaena flos-aquae increased to 5×10~9L~-1, the staining rate would fall to 67% obviously due to cell twisting together.

对细胞密度4×10~7～5×10~8 L~(-1)的水华鱼腥藻，FDA染色效率可达91%以上，但细胞密度增大到5×10~9L~(-1)时，由于细胞易卷曲在一起，FDA染色率下降到67%。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Methods: As the agent of adhibiting tissue section, white latex and glutin were used in the immunohistochemical process of celloidin section.

选用市售白乳胶、明胶作为粘片剂，观察火棉胶切片经过免疫组化染色程序处理后的脱片及染色情况。

Experimental methods: pathological slices: To observe the changes of pathomorphology and measure the circular area of internal elastic lamina , area of neonate intima and vascular lumen, the intimal proliferatory index and percentage, the index of lumen stenosis and positive index of proliferating cell nuclear antigen through the specimens stained by HE and immunohistochemistry.

实验方法：病理切片：进行HE染色和免疫组织化学染色，分别观察病理形态学改变；测定和计算受损血管内弹力膜环绕总面积、新生内膜面积、管腔面积、内膜增生指数、内膜增生百分比、管腔狭窄指数和增殖细胞核抗原阳性指数；观察MMP-2、MMP-3、TIMP-1和PDGF-BB的免疫组化表达。

In the lab, the yellow colorant turns into green when mixed with oxygen in the air!

我们从植物中提取染色剂染衣服，在实验室里，像魔法一样，黄色的染色剂在空气中与氧气结合变成了绿色！

The remaining small part protruded with several crassitude processes which were MAG positive. Ratio of neuron is different(30.2+3.8)%(50.9+2.8)%(58.6+3.3)% and (66.7+4.1)%(P.01). Conclusions is Sodium nitroprusside can inhibit the proliferation and promote the differentiation of spinal neural stem cells into neuronal but not glial cells in vitro.

相差显微镜下大部分神经干细胞伸出多个细长突起，且MAPZ免疫荧光染色增强：部分为有多个短小突起、GFAP阳性表达；部分细胞为少数粗大突起，MAG染色阳性。4组神经元数与三种细胞总数之比，即神经元的比例分别为(30.2士3.8)%、(50.9士2.8)%、(58.6士3.3)%和(66.7士4.1)%，可见硝普钠浓度越高，神经元比例越大(只0.01)。

Methods Transient (15min) brain ischemia was induced by the four-vessel occlusion in Sprague Dawleyrats. The antisense oligodeoxynucleotides of Src and Fyn were used to suppress the expression of Src and Fyn by intracerebroventricular infusion once per day for 3 days before ischemia. After 5 days reperfusion, rats were perfusion-fixed with paraformaldehyde and cresyl violet staining were used to examine the survival of CA1 pyramidal cells of hippocampus.

采用Pulsinelli-Brierley 4动脉阻塞(4-VO)大鼠全脑缺血模型，缺血前连续3d脑室注射Src、Fyn反义寡核苷酸(antisense oligodeoxy-nucletides， AS ODNs)抑制Src、Fyn蛋白的表达后缺血，复灌5d，石蜡切片，焦油紫染色，图像分析测定单位面积内焦油紫染色细胞面积总和，与缺血组及错义寡核苷酸组(missense oligodeoxy-nucletides， MS ODNs)比较进行形态学分析。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。