染色的

Nylon dyeing with acid dyestuffs was carried out with agent NSZ and acetic acid as acidifier individually, dyeing results were compared.

研究了分别以醋酸和染色酸NSZ作为酸剂对锦纶酸性染料染色性能的影响。

Nylon dyeing with acid dyes tuffs was carried out with agent NSZ and acetic acid as acidifier individually,dyeing results were compared.

研究了分别以醋酸和染色酸NSZ作为酸剂对锦纶酸性染料染色性能的影响。

The results showed:(1) the cellule morphous and the disposition in chick embryo of PGCs were coincident no matter stained by PAS or HE staining, and HE staining could disclose the morphologic characteristics more clearly, exactly and completely;(2) genesis of blood island could be observed at the boundary of light and dark region of the extraembryonic blastoderm at about 26 hours;(3) both the blood vessel endothelium cells and free cells of the blood island were differentiated from PGCs. The generating of genuine yolk sac was at about 44-48 hours.

结果表明：①用经典的识别原生殖细胞的PAS染色方法与HE染色所观察到的PGC在鸡胚的分布及细胞形态基本一致，而HE染色较PAS法能更有助于清晰、完整、准确地识别PGC的细胞内部结构特征；②在孵化26小时的鸡胚胚外胚盘的明暗交界处可见血岛的发生，血岛发生的时间比真正的卵黄囊产生时间（大约44-48小时）要早很多；③血岛周围的血管内皮细胞与管腔中游离细胞都来自PGC。

The present studies showed that two cell populations were found in haemocytes: large cell with high granularity and small cell with low granularity by flow cytometry FCM on light scanttering pattern. Two distinct cell types were identified based on phase contrast microscope: one type of cell was dark and dioptric aberration, while the other was bright and dioptric strong. By Giemsa and H.E staining, cytoplasmic staining were heterogeneous and internal particles were obvious in one type of cell, while cytoplastic staining were homogeneous and internal particles were inexistent in the other type of cell. By transmission electron microscope, we found that the mitochondria, Golgi apparatus organelles were rich and internal particles were obvious in one type of cells, and contrary to the another cells.

流式细胞术光散射图谱显示血细胞被分两类，一类为颗粒度高的大细胞，另外一类为颗粒度低的小细胞；相差显微镜观察显示，血细胞可分为胞体暗、折光性差和胞体明亮、折光性强的两类； Giemsa和H.E染色显示细胞分为胞质染色不均一、胞内颗粒明显和胞质染色均一、胞内颗粒不明显的两类；透射电镜超薄切片观察显示，颗粒明显的细胞胞质内线粒体、高尔基体等细胞器较丰富，颗粒不明显的细胞胞质内细胞器较少；负染结果表明血细胞主要分为表面不光滑、突起明显和细胞表面光滑、突起较不明显的两类。

Each of these bundles is called a nucleosome, and many nucleosomes are bound together by the continuing strand of DNA, which forms a string of beads that further coils to form one of two kinds of chromatin, either euchromatin or heterochromatin.

所有细胞核的最显著的特点就是染色体，由DNA和组蛋白所组成的染色质构成，DNA绕行在由4个相似的组蛋白分子结合而形成的柱状的芯上，这一结构称为核小体，核小体之间由连续的DNA链连接，形成串珠样结构，进一步盘曲形成常染色质或异染色质。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

The main research works in this article are as follows:(1) Studying and analysising special algorithms (such as Genetic Algorithms, Neuual Networks and Simulated Annealing Algorithms) based on the proper K-vertex coloring, and applied to the strong vertex-distinguishing total coloring on complete graph on complete graph, then analysises a series of questions as well as the consequences after the application .

本文的主要研究工作如下：(1)分析了基于经典算法(遗传算法、神经网络、模拟退火算法)的正常K-点染色，并把它们应用到完全图的点可区别强全染色中，分析了应用之后出现的一系列问题以及造成的后果，结果表明这些算法是不适合应用在完全图的点可区别强全染色中的。

All tumors were histologically high grade (6 grade III and 10 grade IV). Three tumors showed heterologous elements, 2 osseous, and 1 rhabdomyoblastic. More often scattered than diffuse, S-100 protein staining was noted in 11 of 16 tumors and variable collagen IV staining in 10 of the 16. Immunoreactivity for p53 protein was diffuse and strong in 7 of 11 tumors. Twelve patients died within 17 months to 3 years of diagnosis, 1 was lost to follow-up, 2 are very recent cases, and 2 patients are currently alive, 1 after 2 recurrences, and another with spinal leptomeningeal metastases.

所有病例在组织学上都是高级别（6例III级和10例IV级）。3例肿瘤含有其他成分：2例含骨和1例含 rhabdomyoblastic.S-100蛋白染色显色于16例肿瘤中的11例，IV型胶原染色显色于16例中的10例，这些染色通常分散而不弥散。11例肿瘤中7例的p53蛋白免疫反应弥散且强烈。12例病人死于确诊后的17个月至3年，1例随访失败，2例是最近的病例，还有2例现在还活着，其中1例复发，另1例发生脊髓脑膜转移。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

A manufacturing method for a polarized light film is following: processing the material film (1) of the polyvinyl alcohol resin through the expanding sink (3) as a procedure, processing the material in the dipping sink (4) as a procedure, the dyeing procedure for the dyeing process in the dyeing sink (5) and continuous procedure for the boric acid process in the boric acid sink (6); and processing the single shaft extending for the polarized light film (9) in one procedure at least of the dyeing sink (5) and the boric acid sink (6), the invention processes the polyvinyl alcohol resin film in the direction of the mechanism in the dipping sink (4), which reaches the extending multiplying power above 1.0 fold and below 1.05 folds.

偏振光薄膜的制造方法，使聚乙烯醇系树脂的原料薄膜(1)按顺序通过在膨润槽(3)中的膨润处理工序、在水浸渍槽(4)中的水浸渍处理工序、在染色槽(5)中的染色处理工序以及在硼酸槽(6)中的硼酸处理工序连续进行处理，并且在染色槽(5)以及硼酸槽(6)中的至少一个工序中进行单轴拉伸制造偏振光薄膜(9)时，在水浸渍槽(4)中相对于机械方向处理聚乙烯醇系树脂薄膜，使拉伸倍率达到1倍以上并且在1.05倍以下。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。