染色的

E and TUNEL Besides,early apoptotic cells which were densely green yellowstained or displaying green yellow fragments,late apoptotic cells which were densely cardinal redstained or displaying cardinal red fragments, and cardinal red swollen necrotic cells could be found in the AO/EB doubly stained cells.

AO/EB双染色法还能够显示呈致密浓染的黄绿色染色或出现黄绿色碎片的早期凋亡细胞，呈致密浓染的鲜红色染色或出现鲜红色碎片的晚期凋亡细胞，以及呈鲜红色膨大状的坏死细胞。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

And the repression of NO-induced cell death with Ppbi-1 overexpression in transgenic lines was apparent even at SNP concentrations up to 1mM.In addition work,we found that most of suspension cells treated with 0.5mM SNP can be stained by Sytox green,some PCD morphologic characteristics such as chromatin condensation and margination can be observed.DNA Ladder was also detected with these cells.As to the control and the cell treated with 0.5mMSNP+20μM Est,the above-mentioned characteristics can\'t be detected.

对0.5mM SNP和0.5mM SNP+20μM Est处理的飞廉转基因细胞培养三天后用特异性核染料sytox染色，0.5mMSNP单独处理的细胞大部分核被染色，而且细胞核出现了核凝聚、以及染色质边集等凋亡细胞的特征；提取细胞DNA电泳分析，观察到了DNA LADDER，这说明，该细胞可能已经发生凋亡，而Est诱导Ppbi-1基因表达的细胞均未出现这些凋亡特征。

Disk height index of intervertebral disks on X-ray films, areas and average T2 relaxation time of high intensity zone of disks on T2-weight MRI and histological score on pathological sections （HE staining, Masson staining and safranin O-fast green staining） were measured at 2 months, 4 months and 6 months after operation.

术后2、4、6个月时对正常及模型椎间盘观察X线片上的椎间盘高度指数、MRIT2加权相上的椎间盘高信号区的面积和平均T2弛豫时间、椎间盘纤维环损伤区的形态学变化、组织病理切片（HE染色、Masson三色胶原染色和番红O-固绿染色）椎间盘组织形态学变化、生物化学变化和组织学评分等指标。

Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取从屠宰场获取的猪卵巢中的卵母细胞，在加入和不加hCG和PMSG的mTCMl99培养液中分别培养22小时，地衣红染色发现77.6％的卵母细胞达到MII期；去卵丘细胞后在mTBM中用鲜精或冻精解冻后受精6小时，在NCSU23中培养14小时后固定染色发现受精率分别为87.5％和64.1％；电激活后在NCSU23中培养20小时后固定染色发现原核形成率为94.1％。

The source of the energy for both protoplasmic streaming and migration of the chromatin substance is supplied by ATP which is produced in the process of aerobic respiration.

为了进一步证实假说的可靠性，同时，为了弄清染色质穿壁的途径，穿壁后染色质的命运以及核骨架、细胞骨架与染色质穿壁运动的关系，我们以百合为材料。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

Single factor method were compared to the concentration of dyes, dye bath pH, temperature, Dyeing time to do a series of gradient experiment, the two groups reached on experiments with suitable for dyeing process conditions,such as the best dye pH, suitable dye concentration, Dyeing appropriate time,temperature values of humic acid and fulvic acid were dyeing wool and silk, and which the general situation has deep understanding.

采用单因素对比法分别对染料的浓度、染液pH值、染色温度、染色时间等做了系列梯度实验，得出了两组上染实验的最佳染液pH值、染料合适浓度、适当染色时间、适宜上染温度等工艺条件值，对腐植酸和黄腐酸分别上染羊毛线及真丝绸的大体情况有了很深的了解。

The dyeing fastnesses are identical by microcapsulated and uncapsulated dyes. But the color staining was higher by microcapsulated dyes than uncapsulated dyes.

使用胶囊化的染料与未胶囊化的染料进行热溶染色，织物的染色牢度相同，但前者染色织物的沾色牢度好于后者。

GnRH 1R neurons were found as early as on the first day of culture. During the 1st-3rd days in vitro, they grew fastest. The average growth rates of the perikarya and processes were 45 59μm 2/d and 19 39μm/d, respectively.

培养1d即可见GnRH－IR神经元；1～3d，GnRH－IR神经元生长最快，胞体和突起的平均生长速度分别为45.59μm2／d和19.39μm／d，染色加深；培养7d，GnRH－IR神经元的胞体截面积、胞体染色强度和突起长度均达最高峰，分别为171.21±43.17μm2，0.289±0.034，87.01±22.33μm；培养14d，GnRH－IR神经元的胞体大小和突起长度维持在7d时的水平，但染色减弱。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。