染色的

Observed with transmission electronic microscope,ES-like cell have obvious nucleolus and a little heterochromatin,and many ribosomes in the cytoplasm with much endoplasmic reticulum and mitochondria. A part of cells appeared alveoli on the nuclear envelope,and chromatin was pycnosis and the .

在透射电镜下细胞核仁明显、核异染色质少；细胞质中核糖体较多，内质网、线粒体发达，部分细胞的细胞核膜上出现小泡，染色质边集，染色质形成凋亡小体。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

The denervated extensor digitorum brevis and the extensor hallucis brevis of the rhesus monkey were autotransplanted in the sacrospinal muscle,and they were biopsied at intervals and stained with HE,Bielshowsky and enzyme.

将恒河猴的双足踇、趾短伸肌8块分2组去神经后游离移植于骶棘肌内，定期活检行光镜(HE染色、银染色、酶染色)及电镜观察肌纤维及神经纤维。

Results: Irradiated part of liver showed radiation-induced liver injury tested by H. E.,trichrome and TGF-β1 immunohistochemical staining.

结果：H.E。染色、TGF-β1免疫组化染色和三色染色检测出了照射区肝脏的肝放射性损伤。

Using CCA, karyotype abnormalities were found in 18 (37.5%) of 48 cases, among which complex karyotypes were found in 4 (8.3%) cases, a deletion of chromosome 5/a deletion of the long arm of chromosome 5 (-5/5q-) in 5 (10.4%) cases,-7/7q- in 5 (10.4%) cases, trisomy 8 in 8 (16.6%) cases, 20q- in 2 (4.6%) cases, unconsistent translocations in 3 (6.2%) cases.

细胞遗传学分析检出染色体异常18例(37.5%)，其中复杂异常4例(8.3%)、5号染色体缺失或5号染色体长臂部分缺失5例(10.4%)、7号染色体缺失或7号染色体长臂部分缺失5例(10.4%)、8号染色体增加8例（16.6%）、20号染色体长臂部分缺失2例（4.6%）、不一致的易位3例（6.2%）。

The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

分离解剖耳蜗后，用碘化丙啶染色细胞核、pholloidin染色f-actin，免疫荧光抗体分别染色单链dna、核酸内切酶g和凋亡诱导因子（apoptosis inducing factors， aif），制备耳蜗铺片，激光共聚焦显微镜下观察凋亡和坏死毛细胞内的荧光信号变化。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下，与对照组相比，经姬姆萨染色观察，西咪替丁、尼扎替丁处理的SW480细胞，先发生皱缩，体积变小，异形性减少，细胞变圆数目增多，细胞核变小，染色变淡，核仁数量减少，部分可见染色质边集现象；细胞间连接疏松，随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后，SW480细胞全部变圆，细胞核碎裂，固缩微核形成，部分不再贴壁。

The optimum dyeing conditions for the WP/PP fiber was as the following: the dye temperature was 95℃, the dyeing time was 45min, the concentration of carrier Dilatin POE was 0.8g/L. The color fastness to laundering, perspiration and crocking were excellent after the WP/PP fiber has been dyed with disperse dyes of Foron.

结果表明：与纯PP纤维相比，WP/PP纤维的染色性能有了很大程度的改善，上染率提高1倍以上；优化染色工艺为：染色温度95℃，染色时间45min，载体Dilatin POE的质量浓度0.8g/L； Foron类分散染料染过的WP/PP纤维具有良好皂洗牢度、汗渍牢度和摩擦牢度。

An experiment was designed to investigate the color fastness of poplar veneer after being dyed with acid red GR, acid black ATT, and acid orange Ⅱ.

以酸性大红GR、酸性黑ATT和酸性橙Ⅱ为染色试剂，选取染色温度、染液pH值、固色剂和均染剂的质量分数为工艺参数，进行意杨单板染色试验和颜色坚牢度测定。

Our products are many varieties sale, leading products have DB210, DB211, DB212 series of high temperature and high pressure dyeing machine, ASME-DB series of high-temperature jet dyeing machine overflow, DB-I-type cheese yarn rapid dryer, KS Series of high temperature and high pressure, at normal temperature and pressure jet Jiaosha dyeing machines, high temperature, at room temperature on the prototype, and other products, dyeing and finishing factories at home and abroad is the ideal choice.

本公司产品繁多，品种俱全，主导产品有DB210、DB211、DB212系列高温高压染色机、ASME－DB系列高温溢流喷射染色机，DB－I型筒子纱快速烘干机、KS系列高温高压、常温常压喷射式绞纱染色机，高温、常温小样机等产品，是国内外染整厂家的理想选择。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。