染色的

The nucleolus (3) shows an internal structure. The chromatin is predominately euchromatin with heterochromatin which is typically located close to the nuclear envelope and is discontinuous at the nuclear pores.

可见核仁内的结构（3），核内常染色质为主，异染色质特征性地分布与核膜内表面，核孔处无异染色质分布。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

The composites were implanted into Latissimus dorsi muscles pouches of rabbits.

复合材料首先在兔背阔肌中埋置，预构新生骨组织，并采用ALP染色、Von Kossa染色和HE染色等观察复合材料的成骨过程和组织形态。

3Chondrogenesis was assessed using Alcian blue staining, Toluidine bluestaining, Safranin O/Fast Green staining at 7 and 14 days, still collagenⅡimmunohistochemistry and aggrecan immunofluorescence at 4, 7 and14 days after initial chondrogenic induction of adipose -derivedmesenchymal stem cells.

3观察脂肪间充质干细胞在诱导后的生长增殖情况、形态变化，于诱导7、14天后应用阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色，以及4、7、14天后应用Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光评价其成软骨能力。

The extracellular matrix wasstained positively for Alcian blue, Toluidine blue and Safranin O / FastGreen at 7 and 14 days after initial chondrogenic induction of adipose -derived mesenchymal stem cells, and indicated collagenⅡand aggrecanwere expressed positively.

2脂肪间充质干细胞在高密度的"微团"培养条件下，于诱导7、14天后阿尔新兰染色、甲苯胺兰染色、藩红0／固绿染色阳性，诱导4、7、14天后Ⅱ型胶原蛋白免疫细胞化学、aggrecan免疫荧光阳性表达。

Staining results of ADSCs: The extracellular matrix Alcian blue staining, Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.

脂肪间充质干细胞诱导后的细胞化学染色结果：诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。

In 10.5 day mouse embryo, by the induction of notochord, mesenchymal cells of ventromedial somite differentiate into sclerotome, cells migrate towards notochord, forming perinotochord tube around notochord, the cartilage is till invisible, alcian dying is faintly positive.

椎体区域发生骨化后（E16.5），阿利辛兰染色呈阴性，茜素红染色呈阳性，仅有Ihh在椎体边缘有表达，无PTHrP表达；在椎间盘区域，仍然保持阿利辛兰染色阳性，仅有PTHrP的表达，无Ihh表达。

Four weeks later, the vein grafts were removed ,and HE staining, P/VB staining ,Ⅷ R:Ag staining, and Oil Red "O" staining were performed. Establish hypercholesterolemic rabbit model of vein grafting with PTFE external stent on one side and unexternal stent on the other side.

建立兔的高胆固醇血症模型并在其基础上行动静脉吻合，定期测量兔的血清总胆固醇(TC，4周后行VG的HE染色、VB染色、Ⅷ因子染色、油红&O&染色。

A method of dyeing a polyamide fiber structure characterized by using a polyamide fiber structure which has structural differences along the fiber length direction and dyeing the structure with an anionic reactive dye in a dye bath having a pH of 3 to 8; and a dyed polyamide fiber structure obtained by the above method.

本发明的聚酰胺类纤维构成物的染色方法，是以用纤维纵向上有结构差别的聚酰胺类纤维构成物，经将pH调至3～8的含阴离子性反应性染料的染液染色为特征的，本发明的染色物是由该染色方法制得的聚酰胺类纤维的染色物。

CoCl2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide was used as a H2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apoptotic cells was assessed by propidium iodide stain flow cytometry. The morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species in PC12 cells was measured by DCFH-DA staining and photofluorography.

应用化学性缺氧模拟剂CoCl2在PC12细胞建立化学性缺氧损伤模型；以硫氢化钠作为H2S的供体；应用CCK-8比色法检测细胞存活率；碘化丙啶染色流式细胞技术检测细胞凋亡率；Hoechst33258染色检测细胞凋亡的形态学变化；罗丹明123(Rh123)染色荧光显微镜照相检测细胞线粒体膜电位；2'，7'-二氯荧光黄双乙酸盐染色荧光显微镜照相检测细胞内的活性氧水平。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。