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Material and methods: 18 cases of keratoconus and 7 cases of normal cornea was stained by haematine-eosin, iron stain, alcian blue stain method and observed by transmit electron microscope and scanning electron microscope.

材料与方法:取角膜移植术钻下的圆锥角膜组织18例,无眼病的尸体角膜7例,经苏木精-伊红染色、铁染色、甲苯氨蓝染色、阿尔新蓝染色和透射电镜和扫描电镜观察。

Shenyang Research Institute of Chemical Industry, Shenyang 110021, China Abstract: Spectrophotometric analysis showed that the peak absorbance for food dye of Allura Red and biological dye of Ponceau-G occurred at 501 and 510 nm. In sunlight after 2, 4, 6, 8, 10 hours, the decomposition rates of Allura Red and Ponceau G were between 2.4 to 5.8% and 3.4 to 10.8%, so they are stable in sunlight. The recovery rates on cottons and glasses were very high, so they are easily recoveried from targets. Further studies showed that the recovery rates of two dyes on the leaves of wheat, cabbage, cucumber and tomato were different, so it need previously determine the recovery rate of tracer on the target crop to adjust the result. Field experiment showed it was easy to measure the pesticide spray distribution of high volume spay and low volume spray to use Allura Red and Ponceau-G as tracers.

沈阳化工研究院,沈阳 110021 摘要:光谱分析表明,食品染色剂诱惑红和生物染色剂丽春红-G的最大吸收波长分别为501 nm和510 nm ;在连续2、4、6、8、10 h阳光照射下,分解率分别在2.4%~5.8%和3.4%~10.8%之间,见光稳定性良好;两种染色剂在棉花叶片和玻璃片上的洗脱回收率都很高,说明易于从靶标上洗脱下来;进一步测定表明,两种染色剂在小麦、甘蓝、黄瓜、番茄叶片上的洗脱回收率各不相同,所以对准备进行测试的作物应先做一次洗脱回收率试验,用以校正测试结果;田间应用表明,用两种染色剂做示踪剂,可以方便地测定常量喷雾和低容量喷雾过程中农药在麦田的沉积分布情况。

Toluidine blue staining and typeⅡcollagen immunohistochemistry staining also showed positive results in both groups, but they were more significant in the experimental group.

石蜡切片HE染色可见类似正常软骨的组织学特征,甲苯胺蓝染色及Ⅱ型胶原免疫组化染色阳性,实验组的阳性染色程度较高。

NGFR positive nerve fibres were markedly reduced or absent in muscular layer and submucous layer while the hypertrophic nerve trunks in myenteric and submucosal plexuses showed a sheath of intense staining in the aganglionic colon from patients with HD.

结果 丰富的NGFR染色阳性神经纤维分布于正常结肠环肌层及粘膜下层,少量分布于纵肌层,NGFR染色阳性神经元分布于肌间神经丛及粘膜下神经丛;NGFR 染色阳性神经纤维在HD无肌间神经节细胞肠段肌层及粘膜下层内明显减少或缺如,而肌间肥大的神经干及粘膜下神经干为NGFR染色阳性,并且在其外周可见一强阳性染色带。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜,用茜素红-台盼蓝染色染色行角膜内皮细胞计数;用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变;并于正常对照组角膜比较。

There is significant difference of the anti-VEGF antibody plus LuoShiNeiYiFang group contrast to normal eupotic endometrium. Conclusions. 1. We think endometriosis can impair the patient's physiopsychological health and Health-related Quality of Life by symptoms such as physical pain and the other factors in this study.

正常在位子宫内膜的染色指数最高,内美通组染色指数的均数和手术造模组基本相同,次于正常内膜;罗氏内异方组染色指数更次之;抗VEGF组和抗VEGF+罗氏内异方组染色指数的均数较低;但只有抗VEGF+罗氏内异方组染色指数与正常在位内膜有显著性差异。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明,野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性,而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性,提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

Specific stainings includ silver staining,Nissl bodystaining and modified trichrome staining with improvements.

组化染色包括嗜银染色、尼氏体染色及新的改良三色法染色。

Results There was a similar distributive pattern of Neul, PPCA and β-gal in the inner ear. Neul intense staining was observed in the cochlear spiral ganglion cells, spiral limbus, spiral ligament, vestibular ganglion cells, cristae, maculae hair cells, and weak staining in inner hair cells, outer hair cells, supplying cells of the organ of Corti and stria vascularis. The intense staining of PPCA and β-gal were observed in the spiral ganglion and vestibular ganglion cells, and weak staining in the spiral limbus, spiral ligament, stria vascularis and organ of Corti. The inner ear exhibited no staining when Neul, PPCA and β-gal were deficient, respectively.

Neul最强的染色主要在螺旋神经节细胞、螺旋韧带、螺旋缘、前庭神经节细胞及壶腹嵴、球囊和椭园囊感觉毛细胞,较弱的染色分布于血管纹和Corti器内、外毛细胞及支持细胞;PPCA和β-gal在螺旋神经节和前庭神经节细胞有较强的染色,血管纹、螺旋韧带、螺旋缘和Corti器内、外毛细胞及支持细胞呈较弱的染色反应;各自酶缺乏时内耳免疫染色消失。

Different dyeing processes were carried out on polyester-cotton blended fabrics with separated disperse and direct dye ing,and same bath alkaline dyeing with disperse/ direct dye s respectively.

对涤棉混纺织物分别采用分散染料、直接染料染色,以及分散和直接染料碱性同浴染色,测试染色织物的最大吸收波长λm ax和K/S值等指标,以确定涤棉混纺织物用分散和直接染料碱性同浴染色工艺。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。