染色法

Characterized by quick, simple, exact, and high positive rate, acridine orange staining fluorescence is better than KOH microscopic examination on diagnosis of fungal keratohelcosis.

丫啶橙荧光染色法简便，快速、准确，阳性率高，优于KOH湿片直接镜检法。

Methods Specific endothelial cell markers with immunohistochemistry and microvessel density with morphometry in 28 casas of laryngopharyngeal malignant melanoma.

方法应用免疫组织化学染色法检测28例咽喉部恶性黑色素瘤中血管内皮细胞特异性标记物Ⅷ因子相关抗原（factor FⅧ-related antigen， FⅧ-RAg）和CD31的表达，并通过形态学计量法计数肿瘤内微血管密度。

This experiment take one-day-old chickens as the object of study, we apply cell raise technologyand MTT method, the indirect enzyme-lined immunosorbent assay, the immunity enzymehistochemistry methods andα-naphthyl acetate esterase etc..

本实验以1日龄雏鸡为研究对象，应用细胞培养技术和MTT法、间接酶联免疫吸附实验、免疫酶组织化学和组织酸性α-醋酸萘酯酶染色法等方法。

Methods:Culture of aorta SMC in vitro,abdominal injection medication and drawing blood through ophthalmectomy,cytotoxic crystal violet staining for measurement of TNF, and dependent-cells MTT colorimetry for measurement of IL-6 were all performed.

体外主动脉SMC培养，腹腔注射给药及眼球摘除取血，细胞毒结晶紫染色法测定TNF，依赖细胞株MTT比色法测定IL-6。

The connection with other nucleis of the pallial thickening were studied by retrograde tracer horseradish peroxidase and fluorescent tracer dextran tetramethylrhodamine, Fluoro-ruby.

本研究采用Nissl染色法、golgi -cox法、神经元逆行追踪标记辣根过氧化物和逆行荧光染料葡聚糖四甲基罗丹明方法，分析了蛤蚧皮质加厚区的核团长度、核团体积、细胞大小、细胞形态、细胞密度、初步的亚区分区以及核团的纤维联系，为阐明爬行动物-蛤蚧的视觉通路核团的特征和纤维联系积累形态学和组织学资料。

Methods:By means of IFA and DFA from 1999 to 2000 serological and epidemiological investigations were conducted.

采用间接免疫荧光抗体法和直接荧光抗体染色法，于1999～2000年在平江县选点调查。

Toluidine blue staining procedure showed that the neural complex consisted of cerebral ganglion, neural gland and loose connective tissue; the cerebral ganglion was divided into three parts as cerebral cortex, medullary layer and transitional zone; the neural gland was composed of glandular lobules, closely apposing at ventral side of the cerebral ganglion; the neurons and nerve fibers were colored deeply, making a distinct contrast with the background, while neither the neurons nor the nerve fibers were distinctly colored by Golgi-argentraffin staining procedure.

甲苯胺蓝染色法显示，神经复合体由神经节、神经腺和疏松结缔组织组成；神经节分为皮质部、髓质部和过渡带；神经腺位于神经节背侧面，由腺小叶组成；神经细胞呈深蓝色，神经纤维呈浅蓝色，与背景反差较大。Golgi镀银法显示的结果不理想，神经细胞和神经纤维着色不明显。

Cell apoptosis was detected by TUNEL technique. The expression of Smac/DIABLO protein in cytoplasm and total caspase-3 protein in myocardia tissue was also analyzed by Western blotting.

实验结束后采用原位末端标记染色法检测心肌细胞凋亡，采用Western blotting法检测心肌组织中caspase-3蛋白及胞浆Smac/DIABLO蛋白表达。

The suptum samples from 102 tuberculosis patients and 45 non-tuberculosis patients were detected by fluorescence quantitative pcr and acid-fast stain.

对102例培养涂阳的结核痰液标本和45例非结核感染者的痰标本，应用荧光定量pcr法、痰涂片抗酸染色法检测结核分枝杆菌。

METHODS:Based on the model of human umbilical vein endothelial cell, this study adopted Rose Bengal Stain, cell ELISA, immunocyto-chemical techniques to investigate the effect of berberine on PMN-endothelium adhesion and the expression of cell adhesion molecules.

以小檗碱加IL-1、TNF处理人PMN或人脐静脉内皮细胞后，用蛋白染料染色法研究小檗碱对PMN与HUVEC粘附的作用，用细胞ELISA、APAAP法研究小檗碱对细胞表面粘附分子表达的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。