染色法

Methods The lymphocytes of rats were isolated from the peripheral blood and cultured with sodium arsenite under 37 ℃.The ROS content was detected by DCFH-DA;The content of LPO was detected by fluoresce method.

体外分离大鼠外周血淋巴细胞后，施加处理因素，在37℃条件下恒温培养，用2'7'-二乙酰二氯荧光素染色法检测细胞内的ROS水平，用硫代巴比妥酸荧光法测定细胞内LPO含量。

The enzyme-linked immunosorbent assay was used for the content measurement of myelin basic protein in telencephalon tissue at 1 week, 1 month, 3 months and 6 months after irradiation.

SD大鼠受2、10或30Gy全脑照射后的1周以及1、3和6个月时，采用酶联免疫吸附法测量脑组织中髓鞘碱性蛋白含量、用Luxol fast blue染色法和电镜观察并分析髓鞘的病理学改变。

In this study, after incubating cultured human umb ilical vein endothelial cells with TNF-α for different time, we employed RT-PCR to assay IL-8 mRNA expression and immunocytochemical stain ing to detect NF-κB activation in HUVECs.

本文用TNF-α孵育培养的人脐静脉内皮细胞不同时间后，用RT-PCR法检测内皮细胞内IL-8 mRNA的表达，并用免疫细胞化学染色法检测内皮细胞内NF-κB的激活。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明，PCR-ELISA检出22份阳性，比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高，而20份证实无结核分枝杆菌的标本，几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

Methods 0, 2.5, 5, 10, 15 and 20 μmol/L arsenious acid-treated human hepatocellular carcinoma HepG2 cells were cultured under hypoxia for 8 hours. The cell proliferation and cell viability was assayed by WST-8 and the trypan blue dye exclusion method. The expressions of HIF-1α in human hepatocellular carcinoma HepG2 cells were checked by RT-PCR and Western Blot.

以0、0.25、0.5、1、2和4μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧环境中培养8h；1μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧培养0~10h，WST-8法和台盼蓝染色法分析细胞增殖和活力，采用RT-PCR和Western Blot方法检测肝癌细胞中HIF-1α的表达。

Methods Semen samples were collected from 278 infertile men. WBC in semen was detected through WBC count by microscope.Sperm morphology was evaluated using artificially modified method by Automa ted Sperm Morphology Analyzer.Acrosomal morphology was analysed with modified P apanicolaou staining.

对278例不育患者采用精子形态检测系统下人工修正方法进行精子形态分析，采用精液白细胞计数法检测精液白细胞，采用改良巴氏染色法分析精子顶体形态。

RESULTS: Treated for a same period （3 d）, the inhibitory effect of MG132 on PC12 ceil proliferation enhanced with the increment of the concentration of MG132（0, 1, 2.5, 5, 10, 20 μmot/L）.

用不同浓度的MG132处理PC12细胞3d，通过3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐微量比色法检测MG132对PC12细胞增殖的影响，通过姬姆萨染色法和流式细胞术检测MG132对PC12细胞凋亡的影响。

objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响，联苯胺染色法检测血红蛋白合成情况，应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04～0.09？mmol／l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002～0.01？mmol／l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成，2种单宁化合物(0.01～0.05？mmol／l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成，特里马素i(0.01？mmol／l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

Result:The majority of eosinophils in nasal polyps were activated, and there were no significant differences in the numbers of total eosinophils (Chromotrope 2R positive cells) and activated eosinophils (EG 2 positive cells) between allergic and nonallergic patients.

①Chromotrope 2R特染法结合应用EG2 抗体的免疫组化染色法简便易行，适合临床应用观察鼻息肉中嗜酸性粒细胞的浸润和活化状况；②活化嗜酸性粒细胞在鼻息肉的发病机制中可能起重要作用。

Methods:MTT assay was used to detect PGPG homotypic adhesion and PGmatrix adhesion,and PGHUVEC adhesion was determined by Rose Bengal stain.

小檗碱（5、10 μg/ml）处理PG细胞24小时，采用MTT法观察PG细胞与PG细胞的黏附，PG细胞与细胞外基质（FN和Martrigel）的黏附；用虎红染色法观察PG细胞与人脐静脉内皮细胞的黏附。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。