染色法

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1，利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞，通过Western blot法检测转染细胞Bcl-XL蛋白的表达，流式细胞仪检测处理后细胞的凋亡情况，锥虫蓝染色法计数存活细胞。

Methods Spectrophotometry was used to examine NOS activity and NADPH-diaphorase histochemistry to identify its localization.

NOS的比活性用分光光度法，NOS在组织中的分布用NADPH-黄递酶组化染色法。

Methods:The biopsies of the ICJ and the sigmoid colon were measured by radioimmunoassay and the neuropeptide positive terminals were studied by immunohistochemistry in 15 controls and 28 patients with IBS.

黏膜标本取自 17例正常人、16例腹泻型 IBS患者和 12例便秘型 IBS患者的回盲部、乙状结肠，应用免疫组织化学染色法和放射免疫测定法分别检测 SP、VIP、CGRP。

Methods: Cell proliferation was measured by crystal voilet staining assay. The activity of lactic acid dehydrogenase in the upper layer of culture solution was tested to examine the damage to the cell membrane.3H-TdR was used to study the effect of porphyrin nitrogen mustard on the synthesis of DNA.

用结晶紫染色法测定卟啉氮芥对人肝癌细胞SMMC7721的光动力抑制效应，用测定培养上清乳酸脱氢酶活性方法研究光动力作用对人肝癌细胞SMMC7721细胞膜的作用，用3H-TdR掺入法研究光动力作用对人肝癌细胞SMMC7721DNA合成的影响。

Methods: The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay, and PCNA productions were examined with immunocytechemistry.

以小鼠成纤维细胞株(NIH3T3)为研究对象，采用四氮唑盐比色法测定仙人掌对成纤维细胞增生的影响，采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原的表达。

Coomassie brilliant blue stains and neuhaus stains was respectively used to detect the content of aninohexose and phosphatide.

采用考马斯亮蓝G250染色法和Neuhaus法分别测定大鼠胃黏膜组织中磷脂和氨基己糖的含量。

Using MTT and AO/EB methods, the photosensitization of fullerenols under visible lights with different wave bands were studied.

用MTT法研究了在不同波段可见光和远红外辐照的下的细胞生物效应；用AO/EB荧光染色法测定了富勒醇在可见光辐照下对L02细胞的损伤。

Respectively in faeces, milk and polluted water was gained, that showed its sensitivity was higher than that of conventional PCR, sheather\'s floating and AP-AFS.

应用Nested PCR技术，对奶牛粪便、牛奶、污水等119个样品隐孢子虫感染及污染情况进行了检测，检出率分别为23.29％、11.11％和0.00％，均高于常规PCR、漂浮法和染色法的检测结果。

Enzyme immunoassay ; immunogold silver staining ; protein ; antigen ; microarray ; tracer system

酶免疫测定法；免疫金银染色法；蛋白质；抗原；微阵列；示踪系统

Influence of ganfujian granule on collagen sediment and hepatocyte proliferation were examined by Picric acid Sirus red staining and immunohistochemical staing methods, and analyzed by computer image analysis technique.

建立DEN诱导大鼠肝硬化模型，同时给予肝复健冲剂进行干预，应用天狼红-苦味酸特染法、免疫组化染色法及计算机图像分析技术检测中药对DEN致大鼠肝纤维化过程胶原沉淀及肝细胞增殖特性的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。